Recombinant Cynomolgus Siglec-2/CD22 His-tag Protein, CF
Recombinant Cynomolgus Siglec-2/CD22 His-tag Protein, CF Summary
Product Specifications
Asp20-Arg687, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9864-SL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Immobilized Recombinant Cynomolgus Monkey Siglec‑2/CD22 (Catalog # 9864-SL) supports the adhesion of human red blood cells. The ED50 for this effect is 0.07-0.42 μg/mL.
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2 μg/lane of Recombinant Cynomolgus Monkey Siglec‑2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ~110 kDa.
Reconstitution Calculator
Background: Siglec-2/CD22
Siglecs are sialic acid specific I‑type lectins that are characterized by an extracellular domain (ECD) with an N‑terminal Ig‑like V-type domain followed by varying numbers of Ig‑like C2-type domains (1, 2). Siglec-2, also known as B cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. In humans, two distinct Siglec-2 cDNAs that arise from differential RNA processing of the same gene have been isolated. The predominant Siglec-2 (Isoform CD22-beta) encodes an 847 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, six Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail with four immunoreceptor tyrosine-based inhibition motifs (ITIMs) (3). The variant Siglec-2 (Isoform CD22-alpha) encodes a 647 aa polypeptide missing two Ig-like C2-type domains and has a truncated (23 aa) cytoplasmic tail (4). Within the ECD, cynomolgus Siglec-2 shares 85%, and 55% aa sequence identity with human and mouse Siglec-2, respectively. Siglec-2 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of Siglec-2 with trans ligands on opposing cells is found to be favored over the binding of ligands in cis (5). Consistent with a single ligand-binding region, the first two N-terminal Ig-like domains mediated CD22 adhesion with lymphocytes, neutrophils, monocytes, and erythrocytes (6). Besides its role as an adhesion molecule, Siglec-2 is a co-receptor that physically interacts with B cell receptor (BCR) and is rapidly phosphorylated upon BCR ligation. It negatively regulates BCR signals by recruiting tyrosine phosphatase SHP-1 to its ITIMs. Phosphorylated Siglec-2 can also interact with other intracellular effector proteins such as Syk, PLC gamma, PI3 kinase and Grb-2, suggesting it may play a role in positive signaling (7, 8).
- Varki, A. and T. Angata (2006) Glycobiology 16:1R.
- Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
- Wilson, G.L et al. (1991) J. Exp. Med. 173:137.
- Stamenkovic, I. and B. Seed (1990) Nature 345:74.
- Collins, B.E. et al. (2004) Proc. Natl. Acad. Sci. 101:6104.
- Engel, P. et al. (1995) J Exp Med. 181:1581
- Ravetch, J.V. and L.L. Lanier (2000) Science 290:84.
- Wienands, Y.J. et al. (1999) J. Biol. Chem. 274:18769.
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