Recombinant Cynomolgus/Rhesus Siglec-3/CD33 His Protein, CF

Catalog # Availability Size / Price Qty
11217-SL-050
Recombinant Cynomolgus/Rhesus Siglec-3/CD33 His-tag Protein SDS-PAGE.
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Recombinant Cynomolgus/Rhesus Siglec-3/CD33 His Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Chinese Hamster Ovary cell line, CHO-derived Siglec-3/CD33 protein
Met16-Gly248, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Met16
Predicted Molecular Mass
27 kDa
SDS-PAGE
42-55 kDa, under reducing conditions.

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11217-SL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11217-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus/Rhesus Siglec-3/CD33 His-tag Protein (Catalog # 11217-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-55 kDa.

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Background: Siglec-3/CD33

Sialic acid-binding Ig-like lectin 3 (Siglec-3), also known myeloid cell surface antigen CD33 (CD33), is a I-type (Ig-type) lectin belonging to the sialoadhesin subclass of the immunoglobulin superfamily (1). Siglecs are characterized by an N-terminal Ig-like V-type domain, which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains in the extracellular domain (ECD). Fourteen human and nine mouse Siglecs have been characterized and are divided into 2 families: CD33 related and evolutionarily conserved (1-3). Mature Siglec-3 consists of an ECD with one Ig-like V-type domain and one Ig-like C2 domain, a single transmembrane and a cytoplasmic tail containing two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (3). Within the ECD, cynomolgus/rhesus Siglec-3 shares 89% amino acid sequence identity with human Siglec-3. An isoform of Siglec-3 lacking the N-terminal V-type domain, generated by alternative splicing, has been identified in humans (4). Siglec-3 is a disulfide-linked homodimer expressed on neutrophils, monocytes, macrophages and dendritic cells (2). Each Siglec family member has a distinct preference for binding the various types of sialylated glycans found on the surface of mammalian cells and they most likely evolved to regulate host immune responses via the recognition of self-glycans (5). Siglec-3 may be implicated in the regulation of both the innate but also the adaptive immunity and human Siglec-3 continues to be a therapeutic target for the treatment of acute myeloid leukemia and is a high potential risk factor for Alzheimer's (6). Siglec-3's ligands have yet to be classified, however terminal epitope preferences are conserved between Siglec-3 and Siglec-9 when binding to glycans (7). R&D Systems in-house testing indicates that Siglec-3 binds to LGALS3BP, consistent with the demonstrated functional interactions between other members of these protein families (8).

Long Name
Sialic Acid Binding Ig-like Lectin 3
Entrez Gene IDs
945 (Human); 12489 (Mouse); 102117580 (Cynomolgus Monkey)
Alternate Names
CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33; FLJ00391; gp67; myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec3; Siglec-3; SIGLEC3gp67

Manufacturing Specifications

  1. Bhattacherjee, A. et al. (2021) Mol. Neurodegener. 16:19.
  2. Murch, S. et al. (2020) Medical Hypotheses. 144:110168.
  3. Hernandez-Caselles, T. et al. (2019) J. Immunol. Res. 2019:6032141.
  4. Hernández-Caselles, T. et al. (2006) J Leukoc Biol. 79:46
  5. Paulson, J. et al. (2012) Ann. N. Y. Acad. Sci. 1253:37.
  6. Malik, et al. (2013) J. Neurosci. 33:13320
  7. Wang, S. et al. (2021) Front Mol. Biosci. 8:645999.
  8. Laubli, H. et al. (2014) J. Biol. Chem. 289:33481.

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