Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein, CF

Catalog #: 10408-SR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10408-SR-050

Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Cynomolgus Monkey/Rhesus Macaque IL-6R alpha Fc Chimera (Catalog # 10408-SR) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human IL-6 (Catalog # 7270-IL) binds with an ED₅₀ of 7.5-45 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived IL-6R alpha protein

Cynomolgus Monkey/Rhesus Macaque IL-6R alpha (Leu20-Asp358) Accession # XP_005541720.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005541720.1

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

64 kDa

SDS-PAGE

89-101 kDa, under reducing conditions

Product Datasheets

10408-SR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10408-SR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Cynomolgus/Rhesus IL-6R alpha Fc Chimera (Catalog # 10408-SR) is immobilized at 0.5 µg/mL, 100 µL/well, Recombinant Human IL-6 (Catalog # 7270-IL) binds with an ED₅₀ of 7.5-45 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-6R alpha Fc Chimera (Catalog # 10408-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 89-101 kDa and 170-190 kDa, respectively.

Reconstitution Calculator

Background: IL-6R alpha

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1-4). The cynomolgus IL-6R alpha cDNA encodes a precursor type I transmembrane protein of 468 amino acids (aa), and based on its similarity with human IL-6R alpha it is predicted to contain a 19 aa signal sequence, a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment likely contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSXWS motif. In their extracellular regions, Cynomolgus IL-6R alpha shares 97% aa identity with human IL-6R alpha. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL-6R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6R alpha. It has been documented that elevated soluble IL-6R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn's disease (6, 7).

References