Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His Protein, CF

Catalog #: 10451-EP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10451-EP-050

Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line. The ED₅₀ for this effect is 0.3-1.5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived EpCAM/TROP1 protein
Gln24-Lys265, with a C-terminal 6-His tag

Accession #

NP_001035118.1

N-terminal Sequence
Analysis

Gln24; inferred from deblocking reaction revealing Lys25

Predicted Molecular Mass

28 kDa

SDS-PAGE

30-43 kDa, under reducing conditions.

Product Datasheets

10451-EP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10451-EP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Immobilized Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His Protein (Catalog # 10451-EP) enhances L Cells mouse fibroblast adhesion in the presence of Human Fibronectin. The ED₅₀ for this effect is 0.3-1.5 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His-tag Protein (Catalog # 10451-EP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-43 kDa.

Reconstitution Calculator

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein. Based on its similarity with human EpCAM, cynomolgus/rhesus EpCAM is predicted to consist of a 242 amino acid (aa) extracellular domain with two epidermal growth factor like (EGF like) repeats within the cysteine rich N terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Cynomolgus and human EpCAM share 93% aa sequence identity (1). During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is expressed on basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia but not on normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (2). It is additionally expressed on undifferentiated embryonic stem cells, thymocytes, and dendritic cells (3-5). It is up-regulated on actively proliferating epithelial tissues, during adult liver regeneration, and on many epithelial cell-derived carcinomas (2, 6). EpCAM functions as a homophilic cell adhesion molecule (7). It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1 (8-11) that can interfere with cell adhesion (12, 13). Proteolytic cleavage of EpCAM releases multiple fragments from the ECD as well as a cytoplasmic fragment that can regulate gene transcription (14-16).

References

Strnad, J. et al. (1989) Cancer Res. 49:314.
Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.
Ng, V.Y. et al. (2010) Stem Cells 28:29.
Nelson, A.J. et al. (1996) Eur. J. Immunol. 26:401.
Borkowski, T.A. et al. (1996) Eur. J. Immunol. 26:110.
de Boer, C.J. et al. (1999) J. Pathol. 188:201.
Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
Nubel, T. et al. (2009) Mol. Cancer Res. 7:285.
Kuhn, S. et al. (2007) Mol. Cancer Res. 5:553.
Schmidt, D.S. et al. (2004) Exp. Cell Res. 297:329.
Litvinov, S.V. et al. (1997) J. Cell Biol. 139:1337.
Gaiser, M.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:E889.
Schnell, U. et al. (2013) Biosci. Rep. 33:e00030.
Schon, M.P. et al. (1993) Int. J. Cancer 55:988.
Maetzel, D. et al. (2009) Nat. Cell Biol. 11:162.

Long Name

Epithelial Cell Adhesion Molecule

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Alternate Names

17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A