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Recombinant Cynomolgus Monkey PCSK9 His-tag Protein, CF

Catalog #: 10469-SE Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10469-SE-050
Recombinant Cynomolgus Monkey PCSK9 His-tag Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey PCSK9 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human LDLR His-tag  (Catalog # 2148-LD) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Proprotein Convertase 9/PCSK9 His-tag (Catalog # 10469-SE) binds with an ED50 of 35-280 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Proprotein Convertase 9/PCSK9 protein
Gln31-Gln152 (pro domain) & Ser153-Gln692 (mature form) with a C-terminal 6-His tag
Accession #
EHH49941.1
N-terminal Sequence
Analysis
Ser153 (mature form) & Gln32 (pro domain)
Structure / Form
Mature form & pro domain
Predicted Molecular Mass
14 kDa & 58 kDa
SDS-PAGE
19 kDa and 61 kDa, under reducing conditions

Product Datasheets

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10469-SE

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10469-SE

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity Recombinant Cynomolgus Monkey PCSK9 His-tag Protein Binding Activity View Larger

When Recombinant Human LDLR (2148-LD) is immobilized at 1 μL/mL (100 μL/well), Recombinant Cynomolgus Monkey PCSK9 His-tag (10469-SE) binds with an ED50 of 35-280 ng/mL.

SDS-PAGE Recombinant Cynomolgus Monkey PCSK9 His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey Proprotein Convertase 9/PCSK9 His-tag (Catalog # 10469-SE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19 and 61 kDa under reducing conditions.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Proprotein Convertase 9/PCSK9

PCSK9 (proprotein convertase subtilisin kexin 9), also known as NARC-1, is a member of the proteinase K subfamily of subtilisin-related serine endoproteases. It is highly expressed in the liver, intestine, and kidney and plays an important role in regulating LDL R expression and circulating cholesterol levels (1). PCSK9 is synthesized as precursor protein that is autocatalytically cleaved in the endoplasmic reticulum to generate a 14 kDa prodomain and a 60 kDa catalytic domain (2). Within the secretion pathway, the prodomain remains associated with and functions as a chaperone for the catalytic domain (2). Although the other members of the proprotein convertase family demonstrate activity on several downstream targets, PCSK9 protease activity has only been demonstrated through its autocatalytic processing (3). PCSK9 plays a key role in the regulation of cholesterol metabolism by binding to hepatic LDL R, LRP-1, VLDL R, and Apolipoprotein E R2 and promoting their lysosomal degradation instead of recycling to the plasma membrane (4-7). It can also regulate cholesterol and triglyceride handling in the intestine and adipose tissue (8-10). These reported functions create significant interest in PCSK9 as a pharmacological target in cardiovascular disease (11). PCSK9 has been reported to interact with non-LDR targets as well, including mediators of inflammation and immunological processes (12).

References
  1. Schulz, R. et al. (2015) Basic Res. Cardiol. 110:4.
  2. Benjannet, S. et al. (2004) J. Biol. Chem. 279:48865.
  3. Naureckiene, S. et al. (2003) Arch. Biochem. Biophys. 420:55.
  4. Zhang, D.W. (2007) J. Biol. Chem. 282:18602.
  5. DeVay, R.M. et al. (2013) J. Biol. Chem. 288:10805.
  6. Canuel, M. et al. (2013) PLoS One 8:e64145.
  7. Poirier, S. et al. (2008) J. Biol. Chem. 283:2363.
  8. Levy, E. et al. (2013) Atherosclerosis 227:297.
  9. Le May, C. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:1484.
  10. Roubtsova, A. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:785.
  11. Chen, B. et. al. (2019) Curr.Top Med. Chem. 19:1790.
  12. Dixon, D.L. et. al. (2016) J. Clin. Lipidol. 10:1073.
Entrez Gene IDs
255738 (Human); 100102 (Mouse); 298296 (Rat); 102142788 (Cynomolgus Monkey)
Alternate Names
EC 3.4.21; EC 3.4.21.111; FH3; FH3neural apoptosis regulated convertase 1; FHCL3; HCHOLA3; hypercholesterolemia, autosomal dominant 3; LDLCQ1; NARC1; NARC-1; NARC-1convertase subtilisin/kexin type 9 preproprotein; NARC1EC 3.4.21.-; Neural apoptosis-regulated convertase 1; PC9; PCSK9; Proprotein Convertase 9; proprotein convertase subtilisin/kexin type 9; Subtilisin/kexin-like protease PC9

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