Recombinant Cynomolgus Monkey Klotho beta His Protein, CF

Recombinant Cynomolgus Monkey Klotho beta His Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Klotho beta

Klotho beta (KLB), a divergent structural member of the glycosidase I superfamily, is a type I transmembrane protein expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Klotho beta, along with the closely related Klotho, consists of a large extracellular domain (ECD) containing two glycosidase-like regions, a transmembrane domain, and a short intracellular tail. The two glycosidase-like regions of Klotho beta share high homology to the one beta -glycosidase family yet lack the critical active site Glu residues present in beta -glycosidases necessary for catalytic activity, though physiologically relevant enzymatic activity for Klotho beta has been suggested (2, 3). The ECD of mature cynomologus Klotho beta shares 97% and 80% amino acid sequence identity with the ECD of human and mouse Klotho beta, respectively. Similar to Klotho, Klotho beta helps to regulate multiple metabolic processes in mammals by acting as a co-receptor that facilitates binding between FGF19 subfamily members and their receptors (4). The Klotho beta mediated interaction of FGF19 (FGF15 in mouse) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7- alpha hydroxylase (Cyp7a1) and sterol 12- alpha hydroxylase (Cyp8b1) (5-7). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, up-regulating adipocyte insulin-dependent glucose uptake (5 - 8). Regulation of Klotho beta function has been suggested as a therapy for several cardiometabolic diseases (9).

Related Research Areas

• FGF Family
• Glycobiology-related Enzymes
• Lipid/Cholesterol Metabolism
• Lysosomal Enzymes
• Search Bioprocessing Enzymes
• Search Glycobiology-related Enzymes