Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein, CF

Catalog #: 10306-IR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10306-IR-050

Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey IL-23R Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-23 Protein (Catalog # 1290-IL) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey IL-23R Fc Chimera (Catalog # 10306-IR) that produces 50% of the optimal binding response is found to be approximately 50-250 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey IL-23R protein

Cynomolgus Monkey IL-23R (Gly24-Asp353) Accession # XP_005543141	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005543141

N-terminal Sequence
Analysis

Gly24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

65 kDa

SDS-PAGE

91-102 kDa, under reducing conditions

Product Datasheets

10306-IR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10306-IR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human IL-23 Protein (Catalog # 1290-IL) is immobilized at 1 µg/mL, 100 µL/well, the concentration of Recombinant Cynomolgus Monkey IL-23R Fc Chimera (Catalog # 10306-IR) that produces 50% of the optimal binding response is found to be approximately 50-250 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey IL-23R Fc Chimera (Catalog # 10306-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 91-102 kDa and 180-200 kDa, respectively.

Reconstitution Calculator

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif (6). The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human TH0, TH1 as well as several NK cell lines. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5 (2). The Cynomolgus IL-23 R shares 96%, 71% and 77% amino acid sequence identity to Human, mouse, and rat IL-23 R, respectively.

References