Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF

Catalog #: 10366-ER
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10366-ER-100

Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein Binding Activity

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Product Details

Citations (1)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey EGFR Fc Chimera (Catalog # 10366-ER) is immobilized at 2 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human EGF that produces 50% of the optimal binding response is found to be approximately 4-20 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey EGFR protein

Cynomolgus Monkey EGFR (Leu25-Ser645) Accession # XP_005549616.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

XP_005549616.1

N-terminal Sequence
Analysis

Leu25

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

95 kDa

SDS-PAGE

110-130 kDa, under reducing conditions

Product Datasheets

10366-ER

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10366-ER

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Cynomolgus Monkey EGFR Fc Chimera (Catalog # 10366-ER) is immobilized at 2 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human EGF that produces 50% of the optimal binding response is found to be approximately 4-20 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Cynomolgus Monkey EGFR Fc Chimera (Catalog # 10366-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa and 220-260 kDa, respectively.

Reconstitution Calculator

Background: EGFR

Epidermal growth factor receptor (EGFR) is a member of a subfamily of receptor tyrosine kinases comprised of four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD) containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing (3, 4). The mature ECD of cynomolgus EGFR shares 99% and 90% amino acid sequence identity with human and mouse EGFR, respectively. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (5‑7). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (5-7). EGFR is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs (8).

References

Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
Shilo, B.Z. (2005) Development 132:4017.
Guillaudeau, A. et al. (2012) PLoS One. 7:1.
Reiter J.L. et al. (2001) Genomics 71:1.
Freed, D.M. et al. (2017) Cell. 171:683.
Burgess, A.W. et al. (2003) Mol. Cell 12:541.
Faria, J.A. et al. (2016) BBRC. 478:39.
Lee, C.K. et al. (2017) J. Thoracic Oncology. 12:403.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Related Research Areas

Citation for Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A bispecific ?? T-cell engager targeting EGFR activates a potent V?9V?2 T cell-mediated immune response against EGFR-expressing tumors
Authors: King, LA;Toffoli, EC;Veth, M;Iglesias-Guimarais, V;Slot, MC;Amsen, D;van de Ven, R;Derks, S;Fransen, MF;Tuynman, JB;Riedl, T;Roovers, RC;Adang, AEP;Ruben, JM;Parren, PWHI;de Gruijl, TD;van der Vliet, HJ;
Cancer immunology research
Species: N/A
Sample Types: DNA/Recombinant Protein
Applications: Bioassay