Recombinant Cynomolgus CD48/SLAMF2 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10399-CD-050
Recombinant Cynomolgus CD48/SLAMF2 His-tag Protein Binding Activity
2 Images
Product Details
FAQs
Reviews

Recombinant Cynomolgus CD48/SLAMF2 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinabt Human 2B4/CD244/SLAMF4 Fc Chimera (Catalog # 1039-2B) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Cynomolgus Monkey CD48/SLAMF2 His-tag (Catalog # 10399-CD) that produces 50% of the optimal binding response is approximately 75‑450 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD48/SLAMF2 protein
Gln27-Ser217, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gln27 inferred from enzymatic pyroglutamate treatment revealing Gly28
Predicted Molecular Mass
23 kDa
SDS-PAGE
39-44 kDa, under reducing conditions

Product Datasheets

You must select a language.

x

10399-CD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10399-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Cynomolgus CD48/SLAMF2 His-tag Protein Binding Activity View Larger

When Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera (Catalog # 1039-2B) is immobilized at 0.5 µg/mL, 100 µL/well, the concentration of Recombinant Cynomolgus Monkey CD48/SLAMF2 His-tag (Catalog # 10399-CD) that produces 50% of the optimal binding response is approximately 75-450 ng/mL.

SDS-PAGE Recombinant Cynomolgus CD48/SLAMF2 His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey CD48/SLAMF2 His-tag (Catalog # 10399-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-44 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD48/SLAMF2

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules (1-3). The cynomolgus CD48 cDNA encodes a 241 amino acid (aa) precursor that includes a 26 aa signal sequence, a 191 aa mature protein that contains one Ig-like V-type domain and one Ig-like C2-type domain, and a 23 aa C-terminal propeptide (4). A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients (5). Cynomolgus CD48 shares approximately 90% aa sequence identity with human CD48. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors (4, 6). Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC (7). CD2, 2B4, and heparin sulfate function as CD48 ligands (8‑10). CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4 (8, 11). CD48-CD2 interactions promote T cell activation and class switching to IgG2a in B cells (8, 12). High affinity CD48‑2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation (7, 11, 13, 14). In mouse, CD48-2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor (13). CD48-2B4 mediated inhibition of NK cell activity is distinct from MHC I‑restricted mechanisms (15). CD48 expressed on NK cells is co-activating, whereas CD48 expressed on other cell types inhibits NK cell activation (14). Both CD48 expressing and non‑expressing cells can be targets of NK cell or CTL-mediated lysis (13, 16).

References
  1. Assarsson, E. et al. (2005) J. Immunol. 175:2045.
  2. Bhat, R. et al. (2006) J. Leukocyte Biol. 79:417.
  3. Loertscher, R. and P. Lavery (2002) Transpl. Immunol. 9:93.
  4. Wong, Y.W. et al. (1990) J. Exp. Med. 171:2115.
  5. Smith, G.M. et al. (1997) J. Clin. Immunol. 17:502.
  6. Keil, M.J. et al. (2005) Cell 121:1109.
  7. Morandi, B. et al. (2005) J. Immunol. 175:3690.
  8. Kato, K. et al. (1992) J. Exp. Med. 176:1241.
  9. Latchman, Y. et al. (1998) J. Immunol. 161:5809.
  10. Ianelli, C.J. et al. (1998) J. Biol. Chem. 273:23367.
  11. Messmer, B. et al. (2006) J. Immunol. 176:4646.
  12. Gao, N. et al. (2005) J. Immunol. 174:4113.
  13. Lee, K-M. et al. (2003) J. Immunol. 170:4881.
  14. Lee, K-M. et al. (2006) Blood 107:3181.
  15. McNerney, M.E. et al. (2005) Blood 106:1337.
  16. Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.
Long Name
SLAM Family Member 2
Entrez Gene IDs
962 (Human); 12506 (Mouse); 245962 (Rat); 102134608 (Cynomolgus Monkey)
Alternate Names
BCM1 surface antigen; BCM1; BCM1Leukocyte antigen MEM-102; BLAST; BLAST1; BLAST1TCT.1; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); CD48 antigen; CD48 molecule; CD48; hCD48; mCD48; MEM-102; SLAMF2

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Cynomolgus CD48/SLAMF2 His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Cynomolgus CD48/SLAMF2 His-tag Protein, CF and earn rewards!

Have you used Recombinant Cynomolgus CD48/SLAMF2 His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review