Recombinant Cynomolgus CD48/SLAMF2 Fc Chimera Protein, CF

Catalog #: 10362-CD Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10362-CD-050

Recombinant Cynomolgus CD48/SLAMF2 Fc Chimera Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus CD48/SLAMF2 Fc Chimera Protein, CF Summary

Product Specifications

Accession #

XP_005541296.1

N-terminal Sequence
Analysis

Gln27 inferred from enzymatic pyroglutamate treatment revealing Gly28

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

49 kDa

SDS-PAGE

67-78 kDa, under reducing conditions

Product Datasheets

10362-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10362-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Scientific Data

Binding Activity

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When Recombinant Cynomolgus Monkey CD48/SLAMF2 Fc Chimera (Catalog # 10362-CD) is immobilized at 0.5 µg/mL, 100 µL/well, the concentration of Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera (Catalog # 1039-2B) that produces 50% of the optimal binding response is approximately 10-60 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey CD48/SLAMF2 Fc Chimera (Catalog # 10362-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-78 kDa and 135-155 kDa, respectively.

Reconstitution Calculator

Background: CD48/SLAMF2

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules (1-3). The cynomolgus CD48 cDNA encodes a 241 amino acid (aa) precursor that includes a 26 aa signal sequence, a 191 aa mature protein that contains one Ig-like V-type domain and one Ig-like C2-type domain, and a 23 aa C-terminal propeptide (4). A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients (5). Cynomolgus CD48 shares approximately 90% aa sequence identity with human CD48. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors (4, 6). Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC (7). CD2, 2B4, and heparin sulfate function as CD48 ligands (8‑10). CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4 (8, 11). CD48-CD2 interactions promote T cell activation and class switching to IgG2a in B cells (8, 12). High affinity CD48‑2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation (7, 11, 13, 14). In mouse, CD48-2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor (13). CD48-2B4 mediated inhibition of NK cell activity is distinct from MHC I‑restricted mechanisms (15). CD48 expressed on NK cells is co-activating, whereas CD48 expressed on other cell types inhibits NK cell activation (14). Both CD48 expressing and non‑expressing cells can be targets of NK cell or CTL-mediated lysis (13, 16).

References

Assarsson, E. et al. (2005) J. Immunol. 175:2045.
Bhat, R. et al. (2006) J. Leukocyte Biol. 79:417.
Loertscher, R. and P. Lavery (2002) Transpl. Immunol. 9:93.
Wong, Y.W. et al. (1990) J. Exp. Med. 171:2115.
Smith, G.M. et al. (1997) J. Clin. Immunol. 17:502.
Keil, M.J. et al. (2005) Cell 121:1109.
Morandi, B. et al. (2005) J. Immunol. 175:3690.
Kato, K. et al. (1992) J. Exp. Med. 176:1241.
Latchman, Y. et al. (1998) J. Immunol. 161:5809.
Ianelli, C.J. et al. (1998) J. Biol. Chem. 273:23367.
Messmer, B. et al. (2006) J. Immunol. 176:4646.
Gao, N. et al. (2005) J. Immunol. 174:4113.
Lee, K-M. et al. (2003) J. Immunol. 170:4881.
Lee, K-M. et al. (2006) Blood 107:3181.
McNerney, M.E. et al. (2005) Blood 106:1337.
Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.

Long Name

SLAM Family Member 2

Entrez Gene IDs

962 (Human); 12506 (Mouse); 245962 (Rat); 102134608 (Cynomolgus Monkey)

Alternate Names

BCM1 surface antigen; BCM1; BCM1Leukocyte antigen MEM-102; BLAST; BLAST1; BLAST1TCT.1; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); CD48 antigen; CD48 molecule; CD48; hCD48; mCD48; MEM-102; SLAMF2