Recombinant Cynomolgus CD155/PVR Fc Chimera Protein, CF

Recombinant Cynomolgus CD155/PVR Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). CD155 may play a role in cancer cell invasion and migration, and binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homophilicly (3, 4). The mature human CD155 consists of a 323 amino acid (aa) extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24 aa transmembrane segment, and a 50 aa cytoplasmic tail. The sequence of the cynomolgus CD155/PVR was isolated from a cynomolgus monkey cDNA library and aligns with the sequence for Macaca nemestrina (XP_011734201.1 aa24-339, T77M, V93T, G223S, R328G). CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 5-8). It is preferentially expressed on Th17 cells compared to Th2 cells (9), and its activation promotes the development of Th1 responses (10). On migrating cells, CD155 is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 11). Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM‑1 or CD96 expressing NK cells (1, 6, 12). Binding of monocyte DNAM‑1 to endothelial cell CD155 promotes transendothelial migration (7).

Related Research Areas

• Co-stimulatory and Co-inhibitory Molecules
• Infectious Diseases
• Monocyte Markers
• Nectin and Nectin-like Binding Receptors and Ligands
• Search Immune Checkpoint Targets