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Recombinant Cynomolgus BLAME/SLAMF8 Fc Chimera Protein, CF

Catalog #: 11100-BL Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
11100-BL-050
Recombinant Cynomolgus Monkey BLAME/SLAMF8 Fc Chimera Protein Binding Activity.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus BLAME/SLAMF8 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey BLAME/SLAMF8 Fc Chimera (Catalog #11100-BL) is immobilized at 1.0 µg/mL (100 µL/well), the concentration of Recombinant Cynomolgus Monkey BLAME/SLAMF8 Fc Chimera Biotinylated Protein that produces 50% of the optimal binding response is 0.200-2.00 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey BLAME/SLAMF8 protein
Cynomolgus Monkey BLAME/SLAMF8
(Ala23-Asp233)
Accession # XP_005541356.1		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
XP_005541356.1
N-terminal Sequence
Analysis
Ala23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa
SDS-PAGE
55-70 kDa, under reducing conditions.

Product Datasheets

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11100-BL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11100-BL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Cynomolgus Monkey BLAME/SLAMF8 Fc Chimera Protein (Catalog # 11100-BL) is immobilized at 1.0 µg/mL (100 µL/well), the concentration of Recombinant Cynomolgus Monkey BLAME/SLAMF8 Fc Chimera Biotinylated Protein that produces 50% of the optimal binding response is 0.200-2.00 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey BLAME/SLAMF8 Fc Chimera Protein (Catalog # 11100-BL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-70 kDa and 110-140 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BLAME/SLAMF8

B-lymphocyte activator macrophage expressed (BLAME), also known as SLAMF8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. The SLAM family is comprised of nine surface receptors, expressed mainly on hematopoietic cells, and they have been shown to function as adhesion molecules and modulators of immune responses (1). BLAME, along with SLAMF2 and SLAMF9, are considered atypical SLAM family members due to the low homology in their cytoplasmic domains compared to the rest of the SLAM family (2). Mature cynomologus BLAME consists of an extracellular domain (ECD) with an IgV and an IgC2 domain, a transmembrane segment, and a short cytoplasmic domain. Within the ECD, cynomologus BLAME shares 96% amino acid sequence identity with human BLAME. BLAME is expressed by various myeloid cells, such as neutrophils, macrophages, and dendritic cells (3). BLAME suppresses macrophage function but enhances the growth of neoplastic mast cells via SHP-2 (4). BLAME negatively regulates the activity of PKC-δ, which phosphorylates the p40phox subunit of the NOX2 complex (5). BLAME is abundantly expressed in T cells in pediatric cancers and Epstein-Barr virus-positive gastric cancers and is a potential immunotherapy target for several diseases (6-8). Higher SLAMF8 expression may predict better anti-PD1 immunotherapy efficacy in GI cancer (9).

References
  1. Shachar, I. et al. (2019) Clin. Immunol. 204:23.
  2. Dragovich, M.A. and Mor, A. (2018) Autoimmunity reviews, 17:674.
  3. Wang, G. et al. (2015) PloS one, 10:e0121968.
  4. Sugimoto, A. et al. (2018) Exp. Dermatol. 27:641.
  5. Wang, G. et al. (2012) J. Immunol. 188:5829.
  6. Orentas, R.J. et al. (2012) Front Oncol. 2:194.
  7. Sugimoto, A. et al. (2020) Sci. Rep. 10:2505.
  8. Zhang, Q. et al. (2019) J. Clin. Oncol. 37:e14078.
  9. Zhang Q. et al. (2021) Clin. Transl. Immunology. 10:1347.
Long Name
SLAM Family Member 8
Entrez Gene IDs
56833 (Human); 74748 (Mouse); 289237 (Rat); 102122568 (Cynomolgus Monkey)
Alternate Names
B Lymphocyte Activator Macrophage Expressed; BCM-Like Membrane Protein; BLAME; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; CD353; SBBI42; SLAM Family Member 8; SLAMF8

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