Recombinant Cynomolgus 4-1BB/TNFRSF9 Fc Chimera Protein, CF
Recombinant Cynomolgus 4-1BB/TNFRSF9 Fc Chimera Protein, CF Summary
Product Specifications
Cynomolgus Monkey 4-1BB/TNFRSF9 (Leu24-Gln186) Accession # XP_005544945 | IEGRMD | Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9324-4B
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
![Recombinant Cynomolgus 4-1BB/TNFRSF9 Fc Chimera Protein Bioactivity Recombinant Cynomolgus 4-1BB/TNFRSF9 Fc Chimera Protein Bioactivity](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/protein/41BB_93244B_1497.jpg)
When Recombinant Cynomolgus Monkey 4-1BB/TNFRSF9 Fc Chimera is coated at 0.05 µg/mL, 100 µL/well, Recombinant Human 4-1BB Ligand/TNFSF9 (Catalog # 2295-4L) binds with an ED50 of 0.25-1.25 ng/mL.
Reconstitution Calculator
Background: 4-1BB/TNFRSF9/CD137
4-1BB,
also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane
glycoprotein in the TNF receptor superfamily. 4-1BB functions in the
development and activation of multiple immune cells (1). Mature human 4-1BB
consists of a 163 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine‑rich
repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain (2, 3).
Within the ECD, cynomolgus 4-1BB shares 95%, 57%, and 57% aa sequence identity
with human, mouse, and rat 4-1BB, respectively.
4-1BB is expressed as a
disulfide-linked homodimer on various populations of activated T cell including
CD4+, CD8+, memory CD8+, NKT, and regulatory T cells (4-7) as well as on
myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially
infected osteoblasts (8-11). It binds with high affinity to the transmembrane
4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid
progenitor cells (3, 8). This interaction co-stimulates the proliferation, activation, and/or
survival of the 4-1BB expressing cell (3-7). It can also enhance the activation-induced
cell death of repetitively stimulated T cells (3). Mice lacking 4-1BB show
augmented T cell activation, perhaps due to its absence on regulatory T cells
(12).
4-1BB can associate with OX40 on activated T cells, forming a complex
that responds to either ligand and inhibits Treg and CD8+ T cell proliferation
(13). Reverse signaling through 4-1BB Ligand inhibits the development of
dendritic cells, B cells, and osteoclasts (8, 11) but supports mature dendritic
cell survival and co‑stimulates the proliferation and activation of
mast cells (9, 10). 4-1BB activation enhances CD8+ T cell and NK cell
mediated anti-tumor immunity (14). It also contributes to the development of
inflammation in high fat diet-induced metabolic syndrome (15). Soluble forms of
4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid
arthritis and hematologic cancer patients, respectively (16, 17).
- Wang, C. et al. (2009) Immunol. Rev. 229:192.
- Schwarz, H. et al. (1993) Gene 134:295.
- Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
- Wen, T. et al. (2002) J. Immunol. 168:4897.
- Pulle, G. et al. (2006) J. Immunol. 176:2739.
- Zheng, G. et al. (2004) J. Immunol. 173:2428.
- Kim, D. et al. (2008) J. Immunol. 180:2062.
- Lee, S. et al. (2008) Nat. Immunol.9:917.
- Choi, B.K. et al. (2009) J. Immunol. 182:4107.
- Nishimoto, H. et al. (2005) Blood 106:4241.
- Saito, K. et al. (2004) J. Biol. Chem. 279:13555.
- Lee, S. et al. (2005) J. Immunol. 174:6803.
- Ma, B.Y. et al. (2005) Blood 106:2002.
- Choi, B.K. et al. (2010) J. Immunol. 185:1404.
- Kim, C. et al. (2011) Diabetes 60:3159.
- Michel, J. et al. (1998) Eur. J. Immunol. 28:290.
- Salih, H.R. et al. (2001) J. Immunol. 167:4059.
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