Recombinant Cotton Rat CD8 alpha Protein, CF

Catalog # Availability Size / Price Qty
9648-CD-050
Product Details
FAQs
Supplemental Products
Reviews (1)

Recombinant Cotton Rat CD8 alpha Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived cotton rat CD8 protein
Ser24-Asp182, with a C-terninal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser24
Predicted Molecular Mass
18 kDa
SDS-PAGE
26-32 kDa, reducing conditions

Product Datasheets

You must select a language.

x

9648-CD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9648-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD8

CD8 is a dimeric complex made up of two Ig superfamily members (1). The CD8 alpha chain is a type I transmembrane glycoprotein that can dimerize either with itself forming CD8 alpha alpha, or with a 30-35 kDa CD8 beta chain, forming CD8 alpha beta (2). CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR co-receptor, while CD8 alpha alpha promotes T cell survival and differentiation (1, 3). The mature cotton rat CD8 alpha consists of a 159 amino acid (aa) extracellular domain (ECD), and runs at ~30 kDa under reduced condition. Within the ECD, cotton rat CD8 alpha shares 49%, 47%, and 54% amino acid sequence identity with human, mouse, and rat CD8 alpha, respectively.

References
  1. Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
  2. Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
  3. Cole, D.K. et al. (2012) Immunology 137:139.
Entrez Gene IDs
925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names
CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Loading...

Reviews for Recombinant Cotton Rat CD8 alpha Protein, CF

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Recombinant Cotton Rat CD8 alpha Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant Cotton Rat CD8 alpha Protein, CF
By Leslie Priddy on 04/20/2018
Application: In vivo study