Rat μ Opioid R/OPRM1 Antibody

Catalog #: MAB6866 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6866
MAB6866-SP

Detection of Rat μ Opioid R/OPRM1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat μ Opioid R/OPRM1 Antibody Summary

Specificity

Detects rat μ Opioid R/OPRM1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 677014

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide corresponding to His385-Pro398 of rat μ Opioid R/OPRM1
Accession # P33535

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat μ Opioid R/OPRM1 by Western Blot. Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human µ Opioid R/ OPRM1 Monoclonal Antibody (Catalog # MAB6866) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for µ Opioid R/OPRM1 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mu Opioid R/OPRM1

The mu-type opioid receptor (MOR), also known as OPRM1, is a 60‑70 kDa variably glycosylated G protein-coupled receptor that mediates the biological effects of many alkaloid and peptide opioids including morphine. MOR is primarily expressed on neurons in the brain, spinal cord, and gastrointestinal tract as well as on immune cells. MOR activation induces analgesia, euphoria, sedation, respiratory depression, and reduced intestinal motility. Following agonist binding, MOR is phosphorylated and internalized which contributes to opioid tolerance and desensitization. OPRM1 can form heterdimers with several other 7TM GPCRs including the delta-type Opioid Receptor (DOR), Nociceptin/Orphanin Receptor (ORL1), Neurokinin 1 Receptor (NK1), Somatostatin Receptor 2 (SSTR2), Cannabinoid Receptor 1, CCR5, and the alpha 2A-Adrenergic Receptor (ADRA2A). Rat MOR shares 94% and 98% aa sequence identity with human and mouse MOR, respectively.

Long Name

mu Opioid Receptor

Entrez Gene IDs

4988 (Human); 18390 (Mouse); 25601 (Rat)

Alternate Names

hMOP; KIAA0403; LMOR; MOP; mOpioidR; MOR; MOR1; M-OR-1; MOR-1; MOR1MOR; Mu opiate receptor; mu Opioid R; Mu opioid receptor; mu-type opioid receptor; opioid receptor, mu 1; OPRM; OPRM1