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Rat Nestin Antibody

Catalog #: AF2736
17 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2736
AF2736-SP
Nestin in Rat Cortical Stem Cells.
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Citations (17)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Nestin Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat Nestin
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Nestin
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of rat embyo (E15)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Nestin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry beta -III Tubulin and Nestin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

beta ‑III Tubulin and Nestin in Rat Cortical Stem Cells. beta -III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta -III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195) and 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Nestin antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC). View Larger

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed rat cortical stem cells using Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nestin

Nestin is a 240 kDa class VI intermediate filament protein that was first identified in neuroepithelial stem cells (1, 2). It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% amino acid sequence homology with the mouse protein.

References
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
Entrez Gene IDs
10763 (Human); 18008 (Mouse); 25491 (Rat)
Alternate Names
FLJ21841; NES; Nestin

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Citations for Rat Nestin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Antiproliferative effect of boldine on neural progenitor cells and on glioblastoma cells
    Authors: Jim�nez-Madrona E, Morado-D�az CJ, Talaver�n R et al.
    Frontiers in Neuroscience
  2. Neonatal hyperoxia in mice triggers long-term cognitive deficits via impairments in cerebrovascular function and neurogenesis
    Authors: Marissa A. Lithopoulos, Xavier Toussay, Shumei Zhong, Liqun Xu, Shamimunisa B. Mustafa, Julie Ouellette et al.
    Journal of Clinical Investigation
  3. The Rb/E2F axis is a key regulator of the molecular signatures instructing the quiescent and activated adult neural stem cell state
    Authors: BC Fong, I Chakroun, MA Iqbal, S Paul, J Bastasic, D O'Neil, E Yakubovich, AT Bejjani, N Ahmadi, A Carter, A Clark, G Leone, DS Park, N Ghanem, R Vandenbosc, RS Slack
    Cell Reports, 2022-11-01;41(5):111578.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice
    Authors: O'Shea TM, Ao Y, Wang S et al.
    Nature Communications
  5. Nestin contributes to laser choroidal and retinal neovascularization
    Authors: Sofiane Miloudi, Maud Valensi, Mohamed El Sanharawi, Marc M. Abitbol, Francine Behar-Cohen, Claudine Versaux-Botteri
    Mol Vis
  6. YAP/TAZ maintain the proliferative capacity and structural organization of radial glial cells during brain development
    Authors: Alfonso Lavado, Ruchika Gangwar, Joshua Paré, Shibiao Wan, Yiping Fan, Xinwei Cao
    Developmental Biology
  7. Coordinated regulation of the ribosome and proteasome by PRMT1 in the maintenance of neural stemness in cancer cells and neural stem cells
    Authors: L Chen, M Zhang, L Fang, X Yang, N Cao, L Xu, L Shi, Y Cao
    The Journal of Biological Chemistry, 2021-10-04;297(5):101275.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IF
  8. Identification of Sox2 and NeuN Double-Positive Cells in the Mouse Hypothalamic Arcuate Nucleus and Their Reduction in Number With Aging
    Authors: Ayumu Sugiura, Tatsuhiro Shimizu, Takeshi Kameyama, Tomohiko Maruo, Shin Kedashiro, Muneaki Miyata et al.
    Frontiers in Aging Neuroscience
  9. Microenvironment-responsive immunoregulatory electrospun fibers for promoting nerve function recovery
    Authors: K Xi, Y Gu, J Tang, H Chen, Y Xu, L Wu, F Cai, L Deng, H Yang, Q Shi, W Cui, L Chen
    Nat Commun, 2020-09-09;11(1):4504.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  10. Conditional inactivation of Pen-2 in the developing neocortex leads to rapid switch of apical progenitors to basal progenitors
    Authors: S Cheng, T Liu, Y Hu, Y Xia, J Hou, C Huang, X Zou, J Liang, Y Stone Shi, Y Zheng, J Lu, G Chen
    J. Neurosci., 2019-01-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Differential expression of nuclear lamin subtypes in the neural cells of the adult rat cerebral cortex
    Authors: Y Takamori, Y Hirahara, T Wakabayash, T Mori, T Koike, Y Kataoka, Y Tamura, S Kurebayash, K Kurokawa, H Yamada
    IBRO Rep, 2018-11-05;5(0):99-109.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Injectable polypeptide hydrogels via methionine modification for neural stem cell delivery
    Authors: Wollenberg AL, O'Shea TM, Kim JH et al.
    Biomaterials
  13. Nestin-positive microglia in adult rat cerebral cortex.
    Authors: Takamori Y, Mori T, Wakabayashi T, Nagasaka Y, Matsuzaki T, Yamada H
    Brain Res., 2009-03-21;1270(0):10-8.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  14. Pten deletion in adult neural stem/progenitor cells enhances constitutive neurogenesis.
    Authors: Gregorian C, Nakashima J, Le Belle J, Ohab J, Kim R, Liu A, Smith KB, Groszer M, Garcia AD, Sofroniew MV, Carmichael ST, Kornblum HI, Liu X, Wu H
    J. Neurosci., 2009-02-11;29(6):1874-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Mechanisms of murine lacrimal gland repair after experimentally induced inflammation.
    Authors: Zoukhri D, Fix A, Alroy J, Kublin CL
    Invest. Ophthalmol. Vis. Sci., 2008-06-27;49(10):4399-406.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  16. Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
    Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
    Biotechnol. Bioeng., 2007-08-15;97(6):1617-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Vasopressin mediates mitogenic responses to adrenalectomy in the rat anterior pituitary.
    Authors: Subburaju S, Aguilera G
    Endocrinology, 2007-04-05;148(7):3102-10.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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