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Rat FGF-BP Antibody

Catalog #: AF1413
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1413
AF1413-SP
FGF-BP in Rat Thymus.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat FGF-BP Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat FGF-BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human FGF-BP is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat FGF-BP
Glu21-Cys238
Accession # Q9QY10
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat FGF-BP (Catalog # 1413-FB)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Rat FGF-BP (Catalog # 1413-FB) to immobilized Recombinant Human FGF-basic (Catalog # 233-FB) coated at 5 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry FGF-BP antibody in Rat Thymus by Immunohistochemistry (IHC-Fr). View Larger

FGF-BP in Rat Thymus. FGF-BP was detected in perfusion fixed frozen sections of rat thymus using Rat FGF-BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1413) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-BP

Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2-4). FGF-BP contains a heparin-binding domain (aa 113-146) and a distinct FGF-binding region (aa 197-238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8-11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8-10, 12, 15, 16).

 

References
  1. Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
  2. Aigner, A. et al. (2000) Growth Factors 18:51.
  3. Tassi, E. et al. (2006) Cancer Res. 66:1191.
  4. Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
  5. Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
  6. Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
  7. Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
  8. Beer, H-D. et al. (2005) Oncogene 24:5269.
  9. Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
  10. Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
  11. Kurtz, A. et al. (2004) Neoplasia 6:595.
  12. Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
  13. Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
  14. Kurtz, A. et al. (1997) Oncogene 14:2671.
  15. Aigner, A. et al. (2001) Int. J. Cancer 92:510.
  16. Czubayko, F. et al. (1997) Nat. Med. 3:1137.
Long Name
Fibroblast Growth Factor Binding Protein
Entrez Gene IDs
9982 (Human); 14181 (Mouse); 64535 (Rat)
Alternate Names
17 kDa heparin-binding growth factor-binding protein; FGFBP; FGF-BP; FGFBP1; FGF-BP1; FGFBP-1; FGFBPFGF-binding protein 1; fibroblast growth factor binding protein 1; fibroblast growth factor-binding protein 1; HBp17; HBP1717 kDa HBGF-binding protein; heparin-binding growth factor binding protein

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Citation for Rat FGF-BP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury
    Authors: K Nagashima, T Miwa, H Soumiya, D Ushiro, T Takeda-Kaw, N Tamaoki, S Ishiguro, Y Sato, K Miyamoto, T Ohno, M Osawa, T Kunisada, T Shibata, KI Tezuka, S Furukawa, H Fukumitsu
    Sci Rep, 2017-10-18;7(1):13500.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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