Rat FGF-BP Antibody

Catalog #: MAB1413 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1413
MAB1413-SP

All FGF-BP Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat FGF-BP Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat FGF-BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human FGF-BP is observed.

Source

Monoclonal Mouse IgG_2A Clone # 227239

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat FGF-BP
Glu21-Cys238
Accession # Q9QY10

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat FGF-BP (Catalog # 1413-FB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-BP

Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2‑4). FGF-BP contains a heparin-binding domain (aa 113‑146) and a distinct FGF-binding region (aa 197‑238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8‑11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8‑10, 12, 15, 16).

References

Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
Aigner, A. et al. (2000) Growth Factors 18:51.
Tassi, E. et al. (2006) Cancer Res. 66:1191.
Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
Beer, H.-D. et al. (2005) Oncogene 24:5269.
Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
Kurtz, A. et al. (2004) Neoplasia 6:595.
Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
Kurtz, A. et al. (1997) Oncogene 14:2671.
Aigner, A. et al. (2001) Int. J. Cancer 92:510.
Czubayko, F. et al. (1997) Nat. Med. 3:1137.

Long Name

Fibroblast Growth Factor Binding Protein

Entrez Gene IDs

9982 (Human); 14181 (Mouse); 64535 (Rat)

Alternate Names

17 kDa heparin-binding growth factor-binding protein; FGFBP; FGF-BP; FGFBP1; FGF-BP1; FGFBP-1; FGFBPFGF-binding protein 1; fibroblast growth factor binding protein 1; fibroblast growth factor-binding protein 1; HBp17; HBP1717 kDa HBGF-binding protein; heparin-binding growth factor binding protein