Rat CD43 Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB10388T Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB10388T-100UG
R&D Systems Antibodies
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Rat CD43 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat CD43 in direct ELISAs.
Source
Monoclonal Mouse IgG Clone # W3/13
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Rat thymocyte membrane glycoproteins
Accession # P13838
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Rat splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD43

CD43, also known as Leukosialin, Sialophorin, B-cell differentiation antigen LP-3 and Ly-48, is a type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells. Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific. CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion. In mouse, CD43 is synthesized as a 378 amino acid (aa) precursor that contains a 7 aa signal sequence, a 224 aa extracellular region, a 23 aa TM domain, and a 124 aa cytoplasmic tail. Rat CD43 extracellular region shares a 61% aa sequence identity with the extracellular region in mouse CD43.

Long Name
Cluster of Differentiation 43
Entrez Gene IDs
6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)
Alternate Names
CD43 antigen; CD43; CD43); Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115, leukosialin, CD43); Sialophorin; SPN

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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