Propidium iodide

Biological Activity

Application: flow cytometry, confocal microscopy, fluorescence microscopy.

Used for: apoptosis detection by PI staining, nuclear counterstaining in multicolor fluorescent assays, cell death measuring in a mixed live-cell population by PI staining and flow cytometry.

Properties and Photophysical Data: Propidium iodide fluorescence increases by approximately 20- to 30-fold upon binding to DNA or RNA (through intercalation). When unbound, excitation and emission maxima (λ) are 493 nm and 636 nm, respectively, when bound to DNA, excitation and emission maxima (λ) are 535 nm and 617 nm, respectively.

Optical Data for Propidium iodide

Emission Color	Orange
λabs	535 nm
λem	617 nm
Application	Flow Cytometry, Fluorescence Microscopy, Confocal Microscopy

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. C-reactive protein induces G2/M phase cell cycle arrest and apoptosis in monocytes through the upregulation of B-cell translocation gene 2 expression.
   Kim Y, Ryu J, Ryu M, Lim S, Han K, Lim I, Han K
   FEBS Lett, 2014;588(4):625-31.
2. Megf10 Is a Receptor for C1Q That Mediates Clearance of Apoptotic Cells by Astrocytes
   Joseph El Khoury
   J. Neurosci., 2016;36(19):5185-92.
3. SIRT4 protein suppresses tumor formation in genetic models of Myc-induced B cell lymphoma.
   Jeong et al.
   J.Biol.Chem., 2013;289:4135
4. p53-dependent and p53-independent anticancer effects of different histone deacetylase inhibitors.
   Sonnemann et al.
   Br.J.Cancer, 2014;110:656
5. Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation.
   Yun et al.
   J.Biol.Chem., 2013;289:1491

Product Datasheets