Normal Rabbit IgG Control Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Rabbit IgG Control by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Rabbit Anti-Human/Mouse Pax5/BSAP Monoclonal Antibody (Catalog # MAB3487) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Normal Rabbit IgG Control
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity
Authors: Zhang, H;Xue, K;Li, W;Yang, X;Gou, Y;Su, X;Qian, F;Sun, L;
Nature communications
Species: Transgenic Mouse
Sample Types: In Vivo
Applications: In vivo assay -
Src family kinases-mediated negative regulation of sperm acrosome reaction in chickens (Gallus gallus domesticus)
Authors: C Priyadarsh, R Setiawan, A Tajima, A Asano
PLoS ONE, 2020-11-12;15(11):e0241181.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Effective chimeric antigen receptor T cells against SARS-CoV-2
Authors: Xueyang Guo, Alexandra Kazanova, Stephanie Thurmond, H. Uri Saragovi, Christopher E. Rudd
iScience
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