Mouse VEGFR1/Flt-1 Antibody

Name: Mouse VEGFR1/Flt-1 Antibody
Brand: R&D Systems
SKU: MAB471
Rating: 5 (1 reviews)

Catalog #: MAB471
12 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB471-SP
MAB471-500
MAB471-100

All VEGFR1/Flt-1 Antibodies

Product Details

Citations (12)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGFR1/Flt-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse VEGFR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human VEGFR1, recombinant mouse (rm) VEGFR2, or rmVEGFR3 is observed.

Source

Monoclonal Rat IgG_2A Clone # 141515

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VEGFR1
Ser27-Glu759
Accession # P35969

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse VEGFR1/Flt‑1 Fc Chimera (Catalog # 471-F1)

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of mouse thymus and kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR1/Flt-1

VEGFR1 (also called Flt-1) is one of the five receptor tyrosine kinases (RTKs) (VEGFR1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and Tie-2/Tek define a class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGFR1/Flt-1, VEGFR2/KDR/Flk-1, VEGFR3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGFR1 mRNA encodes a 1333 amino acid (aa) residue precursor with a 22 aa residue signal peptide. Mature VEGFR1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGFR1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGFR1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

References

He, Y. et al. (1999) Molecular Endocrinology 13:537.
Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Alternate Names

EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse VEGFR1/Flt-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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VEGF receptor heterodimers and homodimers are differentially expressed in neuronal and endothelial cell types
Authors: J Sarkar, Y Luo, Q Zhou, E Ivakhnitsk, D Lara, E Katz, MG Sun, V Guaiquil, M Rosenblatt
PLoS ONE, 2022-07-21;17(7):e0269818.
Species: Human, Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation, Western Blot
Pharmacogenetic inhibition of lumbosacral sensory neurons alleviates visceral hypersensitivity in a mouse model of chronic pelvic pain
Authors: AX Xie, N Iguchi, TC Clarkson, AP Malykhina
PLoS ONE, 2022-01-25;17(1):e0262769.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells
Authors: J Falero-Per, N Sheibani, CM Sorenson
PLoS ONE, 2020-05-04;15(5):e0232779.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
1,25(OH)2D3 regulates the proangiogenic activity of pericyte through VDR-mediated modulation of VEGF production and signaling of VEGF and PDGF receptors
Authors: Nasim Jamali, Yong‐Seok Song, Christine M. Sorenson, Nader Sheibani
FASEB BioAdvances
Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells
Authors: J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani
PLoS ONE, 2018-10-29;13(10):e0206756.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
A Functional Role for VEGFR1 Expressed in Peripheral Sensory Neurons in Cancer Pain
Authors: Deepitha Selvaraj, Vijayan Gangadharan, Christoph W. Michalski, Martina Kurejova, Sebastian Stösser, Kshitij Srivastava et al.
Cancer Cell
ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism.
Authors: Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J
J Clin Invest, 2014-04-24;124(6):2696-708.
Species: Mouse
Sample Types: Protein
Applications: Western Blot
Vascular Endothelial Growth Factor Promotes Pericyte Coverage of Brain Capillaries, Improves Cerebral Blood Flow During Subsequent Focal Cerebral Ischemia, and Preserves the Metabolic Penumbra
Authors: Anil Zechariah, Ayman ElAli, Thorsten R. Doeppner, Fengyan Jin, Mohammad R. Hasan, Iris Helfrich et al.
Stroke
Neutrophil gelatinase-associated lipocalin (NGAL) expression is dependent on the tumor-associated sigma-2 receptor S2RPgrmc1.
Authors: Mir SU, Jin L, Craven RJ
J. Biol. Chem., 2012-03-14;287(18):14494-501.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot
Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
Authors: Suryawanshi A, Mulik S, Sharma S
J. Immunol., 2011-02-16;186(6):3653-65.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot

The study of angiogenesis in early rheumatoid arthritis – clinical, immunohistochemical and immunological correlations
Authors: Anca Rosu, Ciurea Ciurea, Simionescu Simionescu, Margaritescu Margaritescu, Musetescu Musetescu, Ciurea Ciurea et al.
J Med Life

Effects of sustained antiangiogenic therapy in multistage prostate cancer in TRAMP model.
Authors: Isayeva T, Chanda D, Kallman L, Eltoum IE, Ponnazhagan S
Cancer Res., 2007-06-15;67(12):5789-97.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P