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Mouse Total MMP-9 ELISA Kit - Quantikine

Catalog #: MMPT90
41 Citations 2 Reviews Datasheet / COA / SDS
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MMPT90
Mouse Total MMP-9 ELISA Standard Curve
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Citations (41)
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Reviews (2)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse Total MMP-9 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.014 ng/mL
Assay Range
0.1 - 5 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant mouse MMP-9 (pro-, active, and TIMP-complexed)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse MMP-9 (total) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total mouse MMP-9 (pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MMP-9 and antibodies raised against the recombinant factor. Natural mouse MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse MMP-9.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 0.239 0.731 1.71 0.211 0.6 1.61
Standard Deviation 0.024 0.037 0.097 0.02 0.049 0.125
CV% 8.4 5.1 5.7 9.6 8.1 7.8

Recovery

The recovery of mouse MMP-9 spiked to 3 levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 94 86-108

Linearity

To assess the linearity of the assay, samples containing high concentrations of mouse MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse Total MMP-9 ELISA Linearity

Scientific Data

N/A Mouse Total MMP-9 ELISA Standard Curve View Larger

Mouse Total MMP-9 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 9
Entrez Gene IDs:
4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names:
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse Total MMP-9 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. The cGAS-STING-YY1 axis accelerates progression of neurodegeneration in a mouse model of Parkinson's disease via LCN2-dependent astrocyte senescence
    Authors: Jiang, SY;Tian, T;Yao, H;Xia, XM;Wang, C;Cao, L;Hu, G;Du, RH;Lu, M;
    Cell death and differentiation
    Species: Mouse
    Sample Types: Cell Culture Supernates, Tissue Homogenates
  2. The Triterpenoid Nrf2 Activator, CDDO-Me, Decreases Neutrophil Senescence in a Murine Model of Joint Damage
    Authors: Amirova, KM;Dimitrova, PA;Leseva, MN;Koycheva, IK;Dinkova-Kostova, AT;Georgiev, MI;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production
    Authors: SY Heazlewood, T Ahmad, B Cao, H Cao, M Domingues, X Sun, CK Heazlewood, S Li, B Williams, M Fulton, JF White, T Nebl, CM Nefzger, JM Polo, BT Kile, F Kraus, MT Ryan, YB Sun, PFM Choong, SL Ellis, ML Anko, SK Nilsson
    Nature Communications, 2023-04-13;14(1):2099.
    Species: Mouse
    Sample Types: Plasma
  4. Pre-Administration of PLX-R18 Cells Protects Mice from Radiation-Induced Hematopoietic Failure and Lethality
    Authors: VP Kumar, S Biswas, GP Holmes-Ham, M Sheleg, S Stone, B Legesse, R Ofir, SP Ghosh
    Genes, 2022-09-28;13(10):.
    Species: Mouse
    Sample Types: Serum
  5. Aerobic exercise inhibits inflammatory response in atherosclerosis via Sestrin1 protein
    Authors: Y Sun, Y Wu, Y Jiang, H Liu
    Experimental gerontology, 2021-10-08;155(0):111581.
    Species: Transgenic Mouse
    Sample Types:
  6. Cdc42-Dependent Transfer of mir301 from Breast Cancer-Derived Extracellular Vesicles Regulates the Matrix Modulating Ability of Astrocytes at the Blood-Brain Barrier
    Authors: G Morad, CC Daisy, HH Otu, TA Libermann, ST Dillon, MA Moses
    Int J Mol Sci, 2020-05-28;21(11):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Topical Application of Hyaluronic Acid-RGD Peptide-Coated Gelatin/Epigallocatechin-3 Gallate (EGCG) Nanoparticles Inhibits Corneal Neovascularization Via Inhibition of VEGF Production
    Authors: T Miyagawa, ZY Chen, CY Chang, KH Chen, YK Wang, GS Liu, CL Tseng
    Pharmaceutics, 2020-04-28;12(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Development of Kaempferol-Loaded Gelatin Nanoparticles for the Treatment of Corneal Neovascularization in Mice
    Authors: YL Chuang, HW Fang, A Ajitsaria, KH Chen, CY Su, GS Liu, CL Tseng
    Pharmaceutics, 2019-11-28;11(12):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Anti-inflammatory signaling by mammary tumor cells mediates prometastatic macrophage polarization in an innovative intraductal mouse model for triple-negative breast cancer
    Authors: J Steenbrugg, K Breyne, K Demeyere, O De Wever, NN Sanders, W Van Den Br, C Colpaert, P Vermeulen, S Van Laere, E Meyer
    J. Exp. Clin. Cancer Res., 2018-08-15;37(1):191.
    Species: Mouse
    Sample Types: Serum
  10. Ginkgo biloba extracts prevent aortic rupture in angiotensin II-infused hypercholesterolemic mice
    Authors: XF Huang, SZ Zhang, YY You, N Zhang, H Lu, A Daugherty, XJ Xie
    Acta Pharmacol. Sin., 2018-05-18;0(0):.
    Species: Mouse
    Sample Types: Serum
  11. Murine neutrophils treated with alphaB-crystallin reduce IL-12p40 production by dendritic cells
    Authors: TM Finlay, AL Palmer, SS Ousman
    Immunology, 2018-04-17;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Vesicular nucleotide transporter mediates ATP release and migration in neutrophils
    Authors: Y Harada, Y Kato, T Miyaji, H Omote, Y Moriyama, M Hiasa
    J. Biol. Chem., 2018-01-23;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. CCAAT/enhancer-binding protein ? overexpression alleviates myocardial remodelling by regulating angiotensin-converting enzyme-2 expression in diabetes
    Authors: Y Tie, C Zhai, Y Zhang, X Qin, F Yu, H Li, M Shan, C Zhang
    J. Cell. Mol. Med., 2017-12-21;0(0):.
    Species: Mouse
    Sample Types: Serum
  14. Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression
    Authors: F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini
    Cancer Res., 2017-07-28;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
    Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
    J Microbiol Immunol Infect, 2017-06-29;0(0):.
    Species: Mouse
    Sample Types: BALF
  16. Zinc metalloproteinase ZmpC suppresses experimental pneumococcal meningitis by inhibiting bacterial invasion of central nervous systems
    Authors: M Yamaguchi, M Nakata, R Sumioka, Y Hirose, S Wada, Y Akeda, T Sumitomo, S Kawabata
    Virulence, 2017-06-05;0(0):0.
    Species: Mouse
    Sample Types: Plasma
  17. Carvedilol attenuates paraquat-induced lung injury by inhibition of proinflammatory cytokines, chemokine MCP-1, NF-?B activation and oxidative stress mediators
    Cytokine, 2016-09-10;88(0):144-153.
    Species: Mouse
    Sample Types: Plasma
  18. beta-Glucuronidase, a Regulator of Lyme Arthritis Severity, Modulates Lysosomal Trafficking and MMP-9 Secretion in Response to Inflammatory Stimuli.
    Authors: Bramwell K, Mock K, Ma Y, Weis J, Teuscher C, Weis J
    J Immunol, 2015-07-13;195(4):1647-56.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
    Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
    Stem Cells Dev, 2015-02-05;24(9):1073-81.
    Species: Mouse
    Sample Types: Serum
  20. A novel molecule Me6TREN promotes angiogenesis via enhancing endothelial progenitor cell mobilization and recruitment.
    Authors: Chen H, Wang S, Zhang J, Ren X, Zhang R, Shi W, Lv Y, Zhou Y, Yan X, Chen L, He L, Zhang B, Nan X, Yue W, Li Y, Pei X
    Sci Rep, 2014-08-28;4(0):6222.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Notch-1 mediated cardiac protection following embryonic and induced pluripotent stem cell transplantation in doxorubicin-induced heart failure.
    Authors: Merino H, Singla D
    PLoS ONE, 2014-07-02;9(7):e101024.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. 12-Hydroxyheptadecatrienoic acid promotes epidermal wound healing by accelerating keratinocyte migration via the BLT2 receptor.
    Authors: Liu M, Saeki K, Matsunobu T, Okuno T, Koga T, Sugimoto Y, Yokoyama C, Nakamizo S, Kabashima K, Narumiya S, Shimizu T, Yokomizo T
    J Exp Med, 2014-05-12;211(6):1063-78.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Eosinophils promote generation and maintenance of immunoglobulin-A-expressing plasma cells and contribute to gut immune homeostasis.
    Authors: Chu V, Beller A, Rausch S, Strandmark J, Zanker M, Arbach O, Kruglov A, Berek C
    Immunity, 2014-04-17;40(4):582-93.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Activation of transcription factor Nrf2 signalling by the sphingosine kinase inhibitor SKI-II is mediated by the formation of Keap1 dimers.
    Authors: Mercado, Nicolas, Kizawa, Yasuo, Ueda, Keitaro, Xiong, Yeping, Kimura, Genki, Moses, Audric, Curtis, Jonathan, Ito, Kazuhiro, Barnes, Peter J
    PLoS ONE, 2014-02-05;9(2):e88168.
    Species: Mouse
    Sample Types: BALF
  25. Pharmacologic inhibition of S-nitrosoglutathione reductase protects against experimental asthma in BALB/c mice through attenuation of both bronchoconstriction and inflammation.
    Authors: Blonder J, Mutka S, Sun X, Qiu J, Green L, Mehra N, Boyanapalli R, Suniga M, Look K, Delany C, Richards J, Looker D, Scoggin C, Rosenthal G
    BMC Pulm Med, 2014-01-10;14(0):3.
    Species: Mouse
    Sample Types: Plasma
  26. Tissue redox activity as a hallmark of carcinogenesis: from early to terminal stages of cancer.
    Authors: Bakalova R, Zhelev Z, Aoki I, Saga T
    Clin Cancer Res, 2013-03-26;19(9):2503-17.
    Species: Mouse
    Sample Types: Plasma
  27. Soluble E-cadherin: a critical oncogene modulating receptor tyrosine kinases, MAPK and PI3K/Akt/mTOR signaling.
    Authors: Brouxhon, S M, Kyrkanides, S, Teng, X, Athar, M, Ghazizadeh, S, Simon, M, O'Banion, M K, Ma, L
    Oncogene, 2013-01-14;33(2):225-35.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. The combination of the histone deacetylase inhibitor vorinostat and synthetic triterpenoids reduces tumorigenesis in mouse models of cancer.
    Authors: Tran, Kim, Risingsong, Renee, Royce, Darlene, Williams, Charlott, Sporn, Michael, Pioli, Patricia, Gediya, Lalji K, Njar, Vincent, Liby, Karen T
    Carcinogenesis, 2012-10-06;34(1):199-210.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. A hypomorphic mutation in the Gfi1 transcriptional repressor results in a novel form of neutropenia.
    Eur J Immunol, 2012-09-01;42(9):2395-408.
    Species: Mouse
    Sample Types: BALF
  30. Role of c-Jun N-terminal protein kinase 1/2 (JNK1/2) in macrophage-mediated MMP-9 production in response to Moraxella catarrhalis lipooligosaccharide (LOS).
    Authors: Hassan F, Ren D, Zhang W
    PLoS ONE, 2012-05-24;7(5):e37912.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. The synthetic triterpenoid CDDO-methyl ester delays estrogen receptor-negative mammary carcinogenesis in polyoma middle T mice.
    Authors: Tran K, Risingsong R, Royce D
    Cancer Prev Res (Phila), 2012-03-08;5(5):726-34.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  32. The effects of low levels of dystrophin on mouse muscle function and pathology.
    Authors: van Putten M, Hulsker M, Nadarajah VD, van Heiningen SH, van Huizen E, van Iterson M, Admiraal P, Messemaker T, den Dunnen JT, 't Hoen PA, Aartsma-Rus A
    PLoS ONE, 2012-02-16;7(2):e31937.
    Species: Mouse
    Sample Types: Serum
  33. Activation of matrix metalloproteinases following anti-Abeta immunotherapy; implications for microhemorrhage occurrence.
    Authors: Wilcock DM, Morgan D, Gordon MN, Taylor TL, Ridnour LA, Wink DA, Colton CA
    J Neuroinflammation, 2011-09-09;8(0):115.
    Species: Mouse
    Sample Types: Tissue Homogenates
  34. Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
    Authors: Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
    J. Neurochem., 2011-01-19;118(4):521-32.
    Species: Mouse
    Sample Types: Cell Lysates
  35. Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner.
    Authors: Liu ZB, Hou YF, Zhu J
    Oncogene, 2010-03-15;29(20):2996-3009.
    Species: Mouse
    Sample Types: Serum
  36. Galectin-3 contributes to neonatal hypoxic-ischemic brain injury.
    Authors: Doverhag C, Hedtjarn M, Poirier F, Mallard C, Hagberg H, Karlsson A, Savman K
    Neurobiol. Dis., 2010-01-04;38(1):36-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
  37. Early protection from burn-induced acute lung injury by deletion of preprotachykinin-A gene.
    Authors: Sio SW, Moochhala S, Lu J
    Am. J. Respir. Crit. Care Med., 2009-10-01;181(1):36-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
  38. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
    Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
    J. Neurochem., 2009-09-24;111(6):1409-24.
    Species: Mouse
    Sample Types: Tissue Homogenates
  39. Inducible nitric oxide synthase deficiency impairs matrix metalloproteinase-9 activity and disrupts leukocyte migration in hepatic ischemia/reperfusion injury.
    Authors: Hamada T, Duarte S, Tsuchihashi S, Busuttil RW, Coito AJ
    Am. J. Pathol., 2009-05-14;174(6):2265-77.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. 15-Epi-lipoxin A(4) inhibits the progression of endometriosis in a murine model.
    Authors: Chen QH, Zhou WD, Pu DM, Huang QS, Li T, Chen QX
    Fertil. Steril., 2009-03-06;93(5):1440-7.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  41. Matrix metalloproteinase 9 facilitates West Nile virus entry into the brain.
    Authors: Wang P, Dai J, Bai F, Kong KF, Wong SJ, Montgomery RR, Madri JA, Fikrig E
    J. Virol., 2008-07-16;82(18):8978-85.
    Species: Mouse
    Sample Types: Serum

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Reviews for Mouse Total MMP-9 Quantikine ELISA Kit

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Mouse Total MMP-9 Quantikine ELISA Kit
By Yan Wang on 11/15/2019
Sample Tested: IC-21 mouse macrophage cell line
Mouse Total MMP-9 Quantikine ELISA Kit
By Anonymous on 05/02/2017
Sample Tested: Lung tissue