Mouse TNFRH3/TNFRSF26 Antibody

Catalog #: AF5330 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5330
AF5330-SP

Detection of Mouse TNFRH3/TNFRSF26 by Western Blot.

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Mouse TNFRH3/TNFRSF26 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TNFRH3/TNFRSF26 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) TNF RI and rmTNF RII is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse TNFRH3/TNFRSF26
Ser20-Lys162
Accession # P83626

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse TNFRH3/TNFRSF26 by Western Blot. Western blot shows lysates of mouse thymus tissue and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse TNFRH3/TNFRSF26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5330) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TNFRH3/TNFRSF26 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry

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Detection of TNFRH3/TNFRSF26 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse TNFRH3/TNFRSF26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5330, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNFRH3/TNFRSF26

TNFRH3 (Tumor necrosis factor receptor homolog 3; also TNFR superfamily member 26) is a 21 kDa (predicted) member of the TNF receptor superfamily. It appears to be concentrated in lymphoid cell types. Mature mouse TNFRH3 is a type I transmembrane protein that is 185 amino acid (aa) in length. It contains a 145 aa extracellular region and a 19 aa cytoplasmic domain. The extracellular region contains three TNFR-Cys repeats that characterize TNF receptors (aa 27‑143). Unlike two related TNF receptor homologs, TNFRH3 does not serve as a TRAIL decoy receptor. No ortholog to mouse TNFRH3 has been reported in human or rat.

Long Name

Tumor Necrosis Factor Receptor Homolog 3

Entrez Gene IDs

244237 (Mouse)

Alternate Names

APO-3;DDR3;DR3;GEF720;LARD;PLEKHG5;TNFRSF12;TNFRSF25;TR3;TRAMP;Tumor necrosis factor receptor superfamily member 25;WSL-1;WSL-LR; TNFRH3; Tnfrsf24; Tnfrsf26