Mouse TLR4 Antibody

Catalog #: MAB27591
3 Citations Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB27591
MAB27591-SP

Detection of TLR4 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

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All TLR4 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TLR4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TLR4 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1203B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TLR4
Asn26-Lys629
Accession # Q9QUK6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunocytochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TLR4 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rabbit Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB27591, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

Immunocytochemistry

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TLR4 in RAW 264.7 Mouse Cell Line. TLR4 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rabbit Anti-Mouse TLR4 Monoclonal Antibody (Catalog # MAB27591) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR4

TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Long Name

Toll-like Receptor 4

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Alternate Names

ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse TLR4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Exosomes mediate sensory hair cell protection in the inner ear
Authors: Andrew M. Breglio, Lindsey A. May, Melanie Barzik, Nora C. Welsh, Shimon P. Francis, Tucker Q. Costain et al.
Journal of Clinical Investigation
Leptospiral LPS escapes mouse TLR4 internalization and TRIF?associated antimicrobial responses through O antigen and associated lipoproteins
Authors: D Bonhomme, I Santecchia, F Vernel-Pau, M Caroff, P Germon, G Murray, B Adler, IG Boneca, C Werts
PLoS Pathog., 2020-08-13;16(8):e1008639.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC
TLR4 Response to LPS Is Reinforced by Urokinase Receptor
Authors: Yulia Kiyan, Sergey Tkachuk, Song Rong, Anna Gorrasi, Pia Ragno, Inna Dumler et al.
Frontiers in Immunology