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Mouse Thrombospondin-4 Antibody

Catalog #: MAB7860 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7860
MAB7860-SP
Thrombospondin-4 in Mouse Connective Tissue.
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Mouse Thrombospondin-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Thrombospondin‑4 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 893655
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Thrombospondin‑4
Gln27-Asn963 (predicted)
Accession # Q9Z1T2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Thrombospondin-4 antibody in Mouse Connective Tissue by Immunohistochemistry (IHC-Fr). View Larger

Thrombospondin-4 in Mouse Connective Tissue. Thrombospondin-4 was detected in perfusion fixed frozen sections of mouse connective tissue using Rat Anti-Mouse Thrombospondin-4 Monoclonal Antibody (Catalog # MAB7860) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-4

Mouse Thrombospondin-4 (TSP-4) is a 120-130 kDa adhesive glycoprotein that belongs to the thrombospondin family of proteins.  It is 963 amino acids (aa) in length and highly modular in structure, containing one Lamnin G-like domain, four EGF-like domains and eight type 3 TSP domains.  It circulates as a disulfide-linked homopentamer, and is up-regulated in the heart in response to ischemic injury and pathology. Mature mouse TSP-4 is 93% aa identical to human TSP4.

Entrez Gene IDs
7060 (Human); 21828 (Mouse)
Alternate Names
THBS4; thrombospondin 4; Thrombospondin4; Thrombospondin-4; TSP4; TSP-4

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