Home / TGF-beta RII / Mouse TGF-beta  RII Biotinylated Antibody

Mouse TGF-beta  RII Biotinylated Antibody

Catalog #: BAF532
9 Citations Datasheet / COA / SDS
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BAF532
Detection of Mouse TGF-beta RII by Flow Cytometry
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TGF-beta  RII Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TGF‑ beta  RII in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) TGF‑ beta  RII and less than 2% cross-reactivity with recombinant mouse TGF‑ beta  RI and rhTGF‑ beta  RIII is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TGF‑ beta  RII and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta  RII
Ile24-Asp184
Accession # Q62312
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TGF-beta  RII Fc Chimera (Catalog # 1600-R2)
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mouse TGF-beta RII by Flow Cytometry View Larger

Detection of Mouse TGF-beta RII by Flow Cytometry Myofibroblastic property of Replic cells. (a) mRNA expression levels of genes related to myofibroblasts in Replic cells cultured with MSCM. In addition to MEFs cultured with DMEM, injured and contralateral kidneys (n = 3 for each) of UUO-treated normal mice were compared to Replic cells with respect to mRNA expression. Twelve biologically independent samples for each cell line were analysed. (b) mRNA expression levels of genes for TGF beta superfamily receptors in Replic cells cultured with MSCM were compared to those in mouse kidneys (n = 3 for ISAM-REC kidneys and WT kidneys) and MEFs. Six biologically independent samples for each cell line were analysed. (c) Flow cytometry of TGF beta R2 expression on the cell surface of Replic cells and MEFs cultured with DMEM. Negative controls (NC) were stained only with APC-conjugated streptavidin. The mean fluorescent intensities are shown (red dotted lines). (d) mRNA expression levels of genes related to myofibroblasts in Replic cells cultured with DMEM or MSCM. Six biologically independent samples for each cell line were analysed. The average expression levels of injured kidneys (a), ISAM-REC kidneys (b), or Replic cells cultured with DMEM (d) were set at 1.0. Error bars are indicated standard errors, and arrows indicate undetectable levels (a,b). *p < 0.05 and **p < 0.01 by multiple comparisons using one-way ANOVA with Tukey-Kramer tests (a,b) or by two-tailed, unpaired Student’s t-tests (d). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31375751), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70 - 85 kDa), and Type III (250 - 350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors (1).

The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

References
  1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.
Long Name
Transforming Growth Factor beta Receptor II
Entrez Gene IDs
7048 (Human); 21813 (Mouse)
Alternate Names
AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

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Citations for Mouse TGF-beta  RII Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. SMAD4 impedes the conversion of NK cells into ILC1-like cells by curtailing non-canonical TGF-beta signaling
    Authors: Victor S Cortez, Tyler K Ulland, Luisa Cervantes-Barragan, Jennifer K Bando, Michelle L Robinette, Qianli Wang et al.
    Nature Immunology
  2. An immortalized cell line derived from renal erythropoietin-producing (REP) cells demonstrates their potential to transform into myofibroblasts
    Authors: Koji Sato, Ikuo Hirano, Hiroki Sekine, Kenichiro Miyauchi, Taku Nakai, Koichiro Kato et al.
    Scientific Reports
  3. TGF-beta Type II Receptor/MCP-5 Axis: At the Crossroad between Joint and Growth Plate Development
    Authors: Lara Longobardi, Tieshi Li, Timothy J. Myers, Lynda O'Rear, Huseyin Ozkan, Ying Li et al.
    Developmental Cell
  4. TGF-beta prevents T follicular helper cell accumulation and B cell autoreactivity.
    Authors: McCarron M, Marie J
    J Clin Invest, 2014-08-26;124(10):4375-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. TGF-beta cytokine signaling promotes CD8+ T cell development and low-affinity CD4+ T cell homeostasis by regulation of interleukin-7 receptor alpha expression.
    Authors: Ouyang W, Oh S, Ma Q, Bivona M, Zhu J, Li M
    Immunity, 2013-08-08;39(2):335-46.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. CD69 regulates type I IFN-induced tolerogenic signals to mucosal CD4 T cells that attenuate their colitogenic potential.
    Authors: Radulovic K, Manta C, Rossini V, Holzmann K, Kestler HA, Wegenka UM, Nakayama T, Niess JH
    J. Immunol., 2012-01-16;188(4):2001-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Fibroblast-specific expression of a kinase-deficient type II transforming growth factor beta (TGFbeta) receptor leads to paradoxical activation of TGFbeta signaling pathways with fibrosis in transgenic mice.
    Authors: Denton CP, Zheng B, Evans LA, Shi-wen X, Ong VH, Fisher I, Lazaridis K, Abraham DJ, Black CM, de Crombrugghe B
    J. Biol. Chem., 2003-04-21;278(27):25109-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. NK1.1+ CD8+ T Cells Escape TGF-beta control and Contribute to Early Microbial Pathogen-response
    Authors: Anne L. Ruiz, Saidi M’Homa Soudja, Cyril Deceneux, Grégoire Lauvau, Julien C. Marie
    Nature Communications
  9. TGF-beta and Eomes control the homeostasis of CD8+ regulatory T cells
    Authors: Shruti Mishra, Wei Liao, Yong Liu, Ming Yang, Chaoyu Ma, Haijing Wu et al.
    Journal of Experimental Medicine

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