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Mouse Serum Amyloid A4 Antibody

Catalog #: MAB4276 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4276
MAB4276-SP
Serum Amyloid A4 in Mouse Brain (Cerebellum).
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Mouse Serum Amyloid A4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serum Amyloid A4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Serum Amyloid A1, rhSerum Amyloid A4, or recombinant mouse Serum Amyloid A1 is observed.
Source
Monoclonal Rat IgG2A Clone # 398104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant mouse Serum Amyloid A4
Asp19-Phe130
Accession # P31532
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Serum Amyloid A4 antibody in Mouse Brain (Cerebellum) by Immunohistochemistry (IHC-Fr). View Larger

Serum Amyloid A4 in Mouse Brain (Cerebellum). Serum Amyloid A4 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Mouse Serum Amyloid A4 Monoclonal Antibody (Catalog # MAB4276) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serum Amyloid A4

Mouse Serum Amyloid A4 (SAA4; also SAA5) is a 14 kDa member of the SAA family of proteins.  It is a constitutively produced apolipoprotein component of HDL that may mediate HDL-VLDL interactions.  Mature mouse SAA4 is 112 amino acids (aa) in length.  Although human SAA4 is glycosylated, mouse SAA4 is not.  Human SAA4 also shows truncation of its Lys-Lys-Tyr C-terminus. The mouse C-terminus is distantly related with a Glu-Lys-Phe tripeptide, but it is unknown if it is proteolytically processed. Over aa 19-130, mouse SAA4 shares 57% and 85% aa identity with mature human and rat SAA4, respectively.   

Entrez Gene IDs
6291 (Human); 20211 (Mouse); 365245 (Rat)
Alternate Names
C-SAA; C-SAACSAAConstitutively expressed serum amyloid A protein; SAA4; SAA5; serum amyloid A-4 protein; Serum Amyloid A4; serum amyloid A4, constitutive

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Mouse Serum Amyloid A4 Antibody
By Anonymous on 12/18/2021
Application: IHC Sample Tested: Brain tissue Species: Mouse
Immunohistochemistry Mouse Serum Amyloid A4 Antibody MAB4276