Mouse Serpin A8/Angiotensinogen Antibody

Catalog #: AF6966
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6966
AF6966-SP

Detection of Mouse Serpin A8/Angiotensinogen by Western Blot.

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Citations (2)

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Mouse Serpin A8/Angiotensinogen Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In direct ELISAs, approximately 24% cross-reactivity with recombinant human Angiotensinogen is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Serpin A1, rmSerpin C1, and rmSerpin F2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Serpin A8/Angiotensinogen
Asp25-Val477
Accession # AAH19496

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Serpin A8/Angiotensinogen by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6966) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Serpin A8/Angiotensinogen at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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Serpin A8/Angiotensinogen in Mouse Intestine. Serpin A8/Angiotensinogen was detected in perfusion fixed frozen sections of mouse intestine using Sheep Anti-Mouse Serpin A8/Angiotensinogen Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6966) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the brush border in intestinal epithelium. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A8/Angiotensinogen

Serpin A8 (serine proteinase inhibitor-clade A8; also angiotensinogen) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II and III. Ang II induces vascoconstriction and aldosterone release by acting on AT1 receptors, while Ang III drives aldosterone release. Ang I can be further processed by MME to generate Ang, a peptide that binds MAS1 on platelets, and promotes the release of NO, an antithrombotic agent. Mature mouse angiotensinogen is 453 aa in length (aa 25-477). It contains Ang I (aa 25-34) that is cleaved to create Ang II (aa 25-32), Ang III (aa 26-32) and Ang I (aa 25-31). Serpin A8/Angiotensinogen may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg. There is an alternative start site five aa upstream of the standard site. Over aa 25-477, mouse serpin A8 shares 86% and 61% aa identity with rat and human serpin A8, respectively.

Entrez Gene IDs

183 (Human); 11606 (Mouse); 24179 (Rat)

Alternate Names

AGT; alpha-1 antiproteinase, antitrypsin; Angiotensin II; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); Angiotensinogen; ANHU; Aogen; FLJ92595; FLJ97926; PAT; pre-angiotensinogen; serine (or cysteine) proteinase inhibitor; Serpin A8; SERPINA8angiotensin I

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Citations for Mouse Serpin A8/Angiotensinogen Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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