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Mouse SCF ELISA Kit - Quantikine

Catalog #: MCK00
27 Citations 1 Review Datasheet / COA / SDS
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MCK00
Mouse SCF/c-kit Ligand ELISA Standard Curve
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Citations (27)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse SCF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
Sensitivity
5 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse SCF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse SCF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse SCF levels in cell culture supernates, serum, and EDTA plasma. It contains E. coli-expressed recombinant mouse SCF and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse SCF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse SCF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 67.2 183 1038 73.4 193 1033
Standard Deviation 2.5 6 30.5 5.3 8.9 47.4
CV% 3.7 3.3 2.9 7.2 4.6 4.6

Recovery

The recovery of mouse SCF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 105 99-113
EDTA Plasma (n=4) 102 84-113
Serum (n=5) 101 92-115

Linearity

To assess the linearity of the assay, five or more samples spiked with various concentrations of mouse SCF in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse SCF/c-kit Ligand ELISA Linearity

Scientific Data

N/A Mouse SCF/c-kit Ligand ELISA Standard Curve View Larger

Mouse SCF/c-kit Ligand ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SCF/c-kit Ligand

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75 - 83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF-R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.

Long Name:
Stem Cell Factor
Entrez Gene IDs:
4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
Alternate Names:
c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse SCF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Haematopoietic stem cell numbers are not solely determined by niche availability
    Authors: Takeishi, S;Marchand, T;Koba, WR;Borger, DK;Xu, C;Guha, C;Bergman, A;Frenette, PS;Gritsman, K;Steidl, U;
    bioRxiv : the preprint server for biology
    Species: Transgenic Mouse, Mouse
    Sample Types: Bone Marrow Lavage Fluid
  2. SCF and IL-33 regulate mouse mast cell phenotypic and functional plasticity supporting a pro-inflammatory microenvironment
    Authors: Molfetta, R;Lecce, M;Milito, ND;Putro, E;Pietropaolo, G;Marangio, C;Scarno, G;Moretti, M;De Smaele, E;Santini, T;Bernardini, G;Sciumè, G;Santoni, A;Paolini, R;
    Cell death & disease
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Endothelial cell-derived stem cell factor promotes lipid accumulation through c-Kit-mediated increase of lipogenic enzymes in brown adipocytes
    Authors: Lee, HJ;Lee, J;Yang, MJ;Kim, YC;Hong, SP;Kim, JM;Hwang, GS;Koh, GY;
    Nature communications
    Species: Mouse
    Sample Types: Plasma, Tissue Homogenates
  4. High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production
    Authors: SY Heazlewood, T Ahmad, B Cao, H Cao, M Domingues, X Sun, CK Heazlewood, S Li, B Williams, M Fulton, JF White, T Nebl, CM Nefzger, JM Polo, BT Kile, F Kraus, MT Ryan, YB Sun, PFM Choong, SL Ellis, ML Anko, SK Nilsson
    Nature Communications, 2023-04-13;14(1):2099.
    Species: Mouse
    Sample Types: Plasma
  5. Low c-Kit expression identifies primitive therapy-resistant CML stem cells
    Authors: M Shah, H Kumar, S Qiu, H Li, M Harris, J He, A Abraham, DK Crossman, A Paterson, RS Welner, R Bhatia
    JCI Insight, 2023-01-10;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. KITLG Promotes Glomerular Endothelial Cell Injury in Diabetic Nephropathy by an Autocrine Effect
    Authors: JC Huang, SC Chen, WA Chang, WW Hung, PH Wu, LY Wu, JM Chang, YL Hsu, YC Tsai
    International Journal of Molecular Sciences, 2022-10-03;23(19):.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates
  7. Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells
    Authors: Y Omatsu, S Aiba, T Maeta, K Higaki, K Aoki, H Watanabe, G Kondoh, R Nishimura, S Takeda, UI Chung, T Nagasawa
    Nature Communications, 2022-05-12;13(1):2654.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Tissue distribution of stem cell factor in adults
    Authors: BM Foster, KL Langsten, A Mansour, L Shi, BA Kerr
    Experimental and molecular pathology, 2021-08-24;122(0):104678.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells
    Authors: AA Hastreiter, GG Dos Santos, EN Makiyama, EWC Santos, P Borelli, RA Fock
    The Journal of nutritional biochemistry, 2021-03-08;0(0):108626.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Pbrm1 Steers Mesenchymal Stromal Cell Osteolineage Differentiation by Integrating PBAF-Dependent Chromatin Remodeling and BMP/TGF-&beta Signaling
    Authors: S Sinha, M Biswas, SS Chatterjee, S Kumar, A Sengupta
    Cell Rep, 2020-04-28;31(4):107570.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Group 2 innate lymphoid cells (ILC2) are regulated by stem cell factor during chronic asthmatic disease
    Authors: W Fonseca, AJ Rasky, C Ptaschinsk, SH Morris, SKK Best, M Phillips, CA Malinczak, NW Lukacs
    Mucosal Immunol, 2019-01-07;12(2):445-456.
    Species: Mouse
    Sample Types: Serum
  12. Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
    Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
    Mol. Cancer Ther., 2018-04-25;0(0):.
    Species: Mouse
    Sample Types: Plasma
  13. First steps to define murine amniotic fluid stem cell microenvironment
    Sci Rep, 2016-11-15;6(0):37080.
    Species: Mouse
    Sample Types: Amniotic Fluid
  14. Protein malnutrition induces bone marrow mesenchymal stem cells commitment to adipogenic differentiation leading to hematopoietic failure.
    Authors: Cunha M, Lima F, Vinolo M, Hastreiter A, Curi R, Borelli P, Fock R
    PLoS ONE, 2013-03-14;8(3):e58872.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.
    Authors: Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M
    Blood, 2011-08-25;118(17):4609-19.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Ectodomain shedding of FLT3 ligand is mediated by TNF-alpha converting enzyme.
    Authors: Horiuchi K, Morioka H, Takaishi H, Akiyama H, Blobel CP, Toyama Y
    J. Immunol., 2009-06-15;182(12):7408-14.
    Species: Mouse
    Sample Types: Serum
  17. Cell-nonautonomous function of Id1 in the hematopoietic progenitor cell niche.
    Authors: Suh HC, Ji M, Gooya J, Lee M, Klarmann KD, Keller JR
    Blood, 2009-05-28;114(6):1186-95.
    Species: Mouse
    Sample Types: Serum
  18. Rosuvastatin reduces atherosclerotic lesions and promotes progenitor cell mobilisation and recruitment in apolipoprotein E knockout mice.
    Authors: Schroeter MR, Humboldt T, Schafer K, Konstantinides S
    Atherosclerosis, 2008-11-25;205(1):63-73.
    Species: Mouse
    Sample Types: Plasma
  19. Direct injection of kit ligand-2 lentivirus improves cardiac repair and rescues mice post-myocardial infarction.
    Authors: Higuchi K, Ayach B, Sato T, Chen M, Devine SP, Rasaiah VI, Dawood F, Yanagisawa T, Tei C, Takenaka T, Liu PP, Medin JA
    Mol. Ther., 2008-11-11;17(2):262-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment.
    Authors: Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH
    Blood, 2008-06-04;112(4):1269-79.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice.
    Authors: Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA
    Stem Cells, 2008-05-15;26(7):1778-86.
    Species: Mouse
    Sample Types: Plasma
  22. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
    Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
    Proc. Natl. Acad. Sci. U.S.A., 2007-10-17;104(43):17069-74.
    Species: Mouse
    Sample Types: Plasma
  23. Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function.
    Authors: Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH
    Blood, 2007-09-20;111(1):219-28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Enalapril increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system.
    Authors: Wang CH, Verma S, Hsieh IC, Chen YJ, Kuo LT, Yang NI, Wang SY, Wu MY, Hsu CM, Cheng CW, Cherng WJ
    J. Mol. Cell. Cardiol., 2006-05-05;41(1):34-43.
    Species: Mouse
    Sample Types: Plasma
  25. A novel polysaccharide of black soybean promotes myelopoiesis and reconstitutes bone marrow after 5-flurouracil- and irradiation-induced myelosuppression.
    Authors: Liao HF, Chen YJ, Yang YC
    Life Sci., 2005-02-25;77(4):400-13.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand.
    Authors: Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S
    Cell, 2002-05-31;109(5):625-37.
    Species: Mouse
    Sample Types: Plasma
  27. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
    Authors: Shih CC, Hu MC, Hu J
    Blood, 2000-03-15;95(6):1957-66.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse SCF Quantikine ELISA Kit
By Anonymous on 07/20/2022
Sample Tested: Cell Culture Media
Mouse SCF Quantikine ELISA Kit MCK00