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Mouse Renin 1 Antibody

Catalog #: AF4277
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4277
AF4277-SP
Renin in Mouse Kidney.
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Product Details
Citations (7)
FAQs
Supplemental Products
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Renin 1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Renin 1 in ELISAs and Western blots. In sandwich immunoassays, approximately 5% cross-reactivity with recombinant rat Renin is observed, and less than 0.2% cross-reactivity with recombinant human Renin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Renin 1 Leu22-Arg402 Accession # P06281
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Renin (Catalog # 4277-AS)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Renin (Catalog # 4277-AS), see our available Western blot detection antibodies

Mouse Renin 1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Renin 1 Biotinylated Antibody (Catalog # BAF4277)

Standard: Recombinant Mouse Renin Protein, CF (Catalog # 4277-AS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Renin antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Renin in Mouse Kidney. Renin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Renin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glomeruli and tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Renin

Mouse Renin is a secreted, 42-47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules are 95% amino acid (aa) identical. Renal Renin (Renin 1) is synthesized as a 381 aa proform (aa 22-402). In the kidney, pro-Renin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro Renin shares 70% and 85% aa sequence identity with human and rat pro Renin, respectively.

Entrez Gene IDs
5972 (Human); 19701 (Mouse)
Alternate Names
angiotensin-forming enzyme; Angiotensinogenase; EC 3.4.23; EC 3.4.23.15; FLJ10761; HNFJ2; Prorenin; REN; renin precursor, renal; Renin

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Citations for Mouse Renin 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Importance of the renal ion channel TRPM6 in the circadian secretion of renin to raise blood pressure
    Authors: Y Funato, D Yamazaki, D Okuzaki, N Yamamoto, H Miki
    Nature Communications, 2021-06-17;12(1):3683.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Vehicle emissions-exposure alters expression of systemic and tissue-specific components of the renin-angiotensin system and promotes outcomes associated with cardiovascular disease and obesity in wild-type C57BL/6 male mice
    Authors: BL Phipps, U Suwannasua, J Lucero, NA Mitchell, AK Lund
    Toxicology reports, 2021-04-15;8(0):846-862.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Renal interstitial fibroblasts coproduce erythropoietin and renin under anaemic conditions
    Authors: K Miyauchi, T Nakai, S Saito, T Yamamoto, K Sato, K Kato, M Nezu, M Miyazaki, S Ito, M Yamamoto, N Suzuki
    EBioMedicine, 2021-01-25;64(0):103209.
    Species: Mouse
    Sample Types: Plasma, Whole Tissue
    Applications: IHC, Western Blot
  4. Ablation of both Cx40 and Panx1 results in similar cardiovascular phenotypes exhibited in Cx40 knockout mice
    Authors: NM Novielli-K, M Jelen, K Barr, LJ Delailo, Q Feng, BE Isakson, R Gros, DW Laird
    Biosci. Rep., 2019-02-27;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Transcriptome-based analysis of kidney gene expression changes associated with diabetes in OVE26 mice, in the presence and absence of losartan treatment.
    Authors: Komers R, Xu B, Fu Y, McClelland A, Kantharidis P, Mittal A, Cohen H, Cohen D
    PLoS ONE, 2014-05-14;9(5):e96987.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Chicken Ovalbumin Upstream Promoter Transcription Factor II Regulates Renin Gene Expression.
    Authors: Mayer S, Roeser M, Lachmann P
    J. Biol. Chem., 2012-05-29;287(29):24483-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron.
    Authors: Pohl M, Kaminski H, Castrop H, Bader M, Himmerkus N, Bleich M, Bachmann S, Theilig F
    J. Biol. Chem., 2010-10-21;285(53):41935-46.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot

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