Home / Wnt-5a / Mouse/Rat Wnt-5a Antibody

Mouse/Rat Wnt-5a Antibody

Catalog #: AF645
55 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF645
AF645-SP
Wnt‑5a in Mouse Embryonic Rib.
15 Images
Product Details
Citations (55)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Wnt-5a Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt‑5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Wnt‑5a peptide
Gln254-Cys334
Accession # P22725
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Lysates of HeLa Human Cervical Epithelial Carcinoma Cells and Mouse Brain (embryo E14)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Wnt‑5a antibody in Mouse Embryonic Rib by Immunohistochemistry (IHC-P). View Larger

Wnt‑5a in Mouse Embryonic Rib. Wnt‑5a was detected in immersion fixed paraffin-embedded sections of mouse embryonic rib using 15 µg/mL Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Wnt-5a antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Wnt‑5a in Mouse Embryo. Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Mouse/Rat Wnt-5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot View Larger

Detection of Mouse/Rat Wnt‑5a by Western Blot. Western blot shows lysates of HeLa Human Cervical Epithelial Carcinoma Cells and Mouse Brain (embryo E14). PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Wnt‑5a at approximately ~42kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Wnt-5a by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse Wnt-5a by Immunocytochemistry/ Immunofluorescence NMDAR activation rapidly increases Wnt5a in cortical cultures. A. Cellular localization of Wnt5a in neurons. Shown are confocal images of primary cortical neurons after double-fluorescent immunostaining with anti-Wnt5a (red) and anti-synapsin I (green) antibodies. The nucleus was stained by DAPI (blue). B. MSG or NMDA stimulation increased Wnt5a protein. Primary cortical neurons (10 DIV) were treated with 10 μΜ MSG or 50 μΜ NMDA for 15 min. Wnt5a protein was detected by Western blotting. Data in the summary graphs (mean ± SEM) were from three independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). C. NMDA receptor-regulated Wnt5a increase. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 100 μΜ DAP5 for 30 min, then incubated with 50 μΜ NMDA for 15 min. Wnt5a and p-P70S6K(included as a marker for translation activation) were detected by Western blotting summarized in the graph (n = 3; *, p < 0.05; **, p < 0.01; One-way ANOVA). D. Dynamic expression of Wnt5a protein after NMDA stimulation. Primary cortical neurons (10 DIV) were treated with 50 μΜ NMDA for 0, 5, 15, 30 and 60 min followed by Western blotting analysis of Wnt5a (n = 3; *, P < 0.05; **, p < 0.01; One-way ANOVA). E. NMDA-induced Wnt5a protein secretion. Primary cortical neurons (10 DIV) were treated with 50 μΜ NMDA for 0, 2, 4, 8, 16 and 32 min, and Wnt5a protein in the media was concentrated and detected on immunoblots. Image collected and cropped by CiteAb from the following open publication (https://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Wnt-5a by Western Blot View Larger

Detection of Mouse Wnt-5a by Western Blot NMDAR activation rapidly increases Wnt5a in cortical cultures. A. Cellular localization of Wnt5a in neurons. Shown are confocal images of primary cortical neurons after double-fluorescent immunostaining with anti-Wnt5a (red) and anti-synapsin I (green) antibodies. The nucleus was stained by DAPI (blue). B. MSG or NMDA stimulation increased Wnt5a protein. Primary cortical neurons (10 DIV) were treated with 10 μΜ MSG or 50 μΜ NMDA for 15 min. Wnt5a protein was detected by Western blotting. Data in the summary graphs (mean ± SEM) were from three independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). C. NMDA receptor-regulated Wnt5a increase. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 100 μΜ DAP5 for 30 min, then incubated with 50 μΜ NMDA for 15 min. Wnt5a and p-P70S6K(included as a marker for translation activation) were detected by Western blotting summarized in the graph (n = 3; *, p < 0.05; **, p < 0.01; One-way ANOVA). D. Dynamic expression of Wnt5a protein after NMDA stimulation. Primary cortical neurons (10 DIV) were treated with 50 μΜ NMDA for 0, 5, 15, 30 and 60 min followed by Western blotting analysis of Wnt5a (n = 3; *, P < 0.05; **, p < 0.01; One-way ANOVA). E. NMDA-induced Wnt5a protein secretion. Primary cortical neurons (10 DIV) were treated with 50 μΜ NMDA for 0, 2, 4, 8, 16 and 32 min, and Wnt5a protein in the media was concentrated and detected on immunoblots. Image collected and cropped by CiteAb from the following open publication (https://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Wnt-5a by Western Blot View Larger

Detection of Mouse Wnt-5a by Western Blot NMDAR activation stimulates Wnt5a protein synthesis via the MAPK signaling pathway. A. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μM PD98059 (PD98) for 30 min, and then stimulated with 50 μΜ NMDA for 15 min. Wnt5a protein was measured by Western blotting and quantified data were presented in graphs (mean ± SEM; n = 3, *p < 0.05; One-way ANOVA). B. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μM U0126 for 30 min, followed by 50 μΜ NMDA for 15 min. Graphs (mean ± SEM) are from three independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). Image collected and cropped by CiteAb from the following open publication (https://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Human Wnt-5a by Western Blot View Larger

Detection of Human Wnt-5a by Western Blot Inhibition of Mov10 increases Wnt5a secretion and Ror2-dependent cell invasion. (a) Wnt5a secretion in conditioned media (CM) from UACC903 melanoma cells compared with whole-cell lysate (WCL) from control and Mov10 shRNA-expressing cells. (b) Wnt5a secretion in UACC903 cells expressing a Wnt5a transgene. (c) Wnt3a secretion in UACC903 cells expressing a Wnt3a transgene. (d) Secretion of fibronectin (FN) from UACC903 cells expressing control and Mov10 shRNA. (e) Collagen invasion of WM239A melanoma cells expressing two Mov10 shRNA constructs. (f) Quantification of invasion assay in (a) Student's t-test, compared with shControl, shMov10-1 P=2.29 × 10−11, shMov10-2 P=3.95 × 10−10. (g) Collagen invasion assay of cells expressing Ror2 shRNA and Mov10 shRNA (see Supplementary Figure S2). (h) Quantification of invasion assay in (d). Student's t-test, shMov10+shCtr vs shCtr P=3.07 × 10−11, shMov10+shRor2-1 vs shMov10+shCtr P=9.02 × 10−10, shMov10+shRor2-2 vs shMov10+shCtr P=1.42 × 10−11. Error bars indicate s.d. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/oncsis201515), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Wnt-5a by Western Blot View Larger

Detection of Mouse Wnt-5a by Western Blot NMDAR activation stimulates Wnt5a protein synthesis via the MAPK signaling pathway. A. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μM PD98059 (PD98) for 30 min, and then stimulated with 50 μΜ NMDA for 15 min. Wnt5a protein was measured by Western blotting and quantified data were presented in graphs (mean ± SEM; n = 3, *p < 0.05; One-way ANOVA). B. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μM U0126 for 30 min, followed by 50 μΜ NMDA for 15 min. Graphs (mean ± SEM) are from three independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). Image collected and cropped by CiteAb from the following open publication (https://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Wnt-5a by Western Blot View Larger

Detection of Mouse Wnt-5a by Western Blot NMDAR activation rapidly increases Wnt5a in cortical cultures. A. Cellular localization of Wnt5a in neurons. Shown are confocal images of primary cortical neurons after double-fluorescent immunostaining with anti-Wnt5a (red) and anti-synapsin I (green) antibodies. The nucleus was stained by DAPI (blue). B. MSG or NMDA stimulation increased Wnt5a protein. Primary cortical neurons (10 DIV) were treated with 10 μΜ MSG or 50 μΜ NMDA for 15 min. Wnt5a protein was detected by Western blotting. Data in the summary graphs (mean ± SEM) were from three independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). C. NMDA receptor-regulated Wnt5a increase. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 100 μΜ DAP5 for 30 min, then incubated with 50 μΜ NMDA for 15 min. Wnt5a and p-P70S6K(included as a marker for translation activation) were detected by Western blotting summarized in the graph (n = 3; *, p < 0.05; **, p < 0.01; One-way ANOVA). D. Dynamic expression of Wnt5a protein after NMDA stimulation. Primary cortical neurons (10 DIV) were treated with 50 μΜ NMDA for 0, 5, 15, 30 and 60 min followed by Western blotting analysis of Wnt5a (n = 3; *, P < 0.05; **, p < 0.01; One-way ANOVA). E. NMDA-induced Wnt5a protein secretion. Primary cortical neurons (10 DIV) were treated with 50 μΜ NMDA for 0, 2, 4, 8, 16 and 32 min, and Wnt5a protein in the media was concentrated and detected on immunoblots. Image collected and cropped by CiteAb from the following open publication (https://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Wnt-5a by Western Blot View Larger

Detection of Mouse Wnt-5a by Western Blot NMDAR activation rapidly increases Wnt5a in cortical cultures. A. Cellular localization of Wnt5a in neurons. Shown are confocal images of primary cortical neurons after double-fluorescent immunostaining with anti-Wnt5a (red) and anti-synapsin I (green) antibodies. The nucleus was stained by DAPI (blue). B. MSG or NMDA stimulation increased Wnt5a protein. Primary cortical neurons (10 DIV) were treated with 10 μΜ MSG or 50 μΜ NMDA for 15 min. Wnt5a protein was detected by Western blotting. Data in the summary graphs (mean ± SEM) were from three independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). C. NMDA receptor-regulated Wnt5a increase. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 100 μΜ DAP5 for 30 min, then incubated with 50 μΜ NMDA for 15 min. Wnt5a and p-P70S6K(included as a marker for translation activation) were detected by Western blotting summarized in the graph (n = 3; *, p < 0.05; **, p < 0.01; One-way ANOVA). D. Dynamic expression of Wnt5a protein after NMDA stimulation. Primary cortical neurons (10 DIV) were treated with 50 μΜ NMDA for 0, 5, 15, 30 and 60 min followed by Western blotting analysis of Wnt5a (n = 3; *, P < 0.05; **, p < 0.01; One-way ANOVA). E. NMDA-induced Wnt5a protein secretion. Primary cortical neurons (10 DIV) were treated with 50 μΜ NMDA for 0, 2, 4, 8, 16 and 32 min, and Wnt5a protein in the media was concentrated and detected on immunoblots. Image collected and cropped by CiteAb from the following open publication (https://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse Wnt-5a by Immunohistochemistry View Larger

Detection of Mouse Wnt-5a by Immunohistochemistry PGC depletion in Ror2 and Wnt5a mutants.(A–C) PGCs were visualized by wholemount SSEA1 immunostaining with SSEA1 antibody in e10.25 WT, Ror2, and Wnt5a embryos. (E–G) Gonadal ridges from e11.5 stained with GCNA, and (I–K) e12.5 male gonads stained with GCNA antibody. The caudal end is down in all images. (D, H, L) Quantification of PGCs in the entire e10.25 embryo, e11.5 and e12.5 gonads from confocal stacks, with individuals denoted as WT (diamond), Ror2Y324C mutants (triangle), and Wnt5a null (circle), and means indicated as bars. Consistent with appearances, a significant reduction of PGCs was observed in Wnt5a and from e11.5 onward in Ror2Y324C. We noted no significant difference in the number of PGCs between XX and XY gonads at e12.5 (not shown). Results of the Student's t-test are indicated, * p<0.05, **, p<0.01, ***p<0.001. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pgen.1002428), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Wnt-5a by Western Blot View Larger

Detection of Mouse Wnt-5a by Western Blot NMDAR-elicited Wnt5a increase requires translation but not transcription. A. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μΜ anisomycin for 30 min, and then incubated with 50 μΜ NMDA for 15 min, followed by Wnt5a immunoblotting. The Graph is a summary of three independent experiments (**, p < 0.01; One-way ANOVA). B. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μΜ actinomycin D for 30 min, followed by addition of 50 μΜ NMDA for 15 min. Wnt5a protein was detected by Western blotting and quantified. The Graph is a summary of four independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). C. Primary cortical neurons (10 DIV) were treated with vehicle (Control) or 50 μΜ NMDA for 15 min. Wnt5a mRNA was quantified by Real-time RT-PCR (qPCR). The summary graph is from three independent experiments (40 cycles, CT values: 25.1 ± 0.5/control vs. 25.6 ± 0.3/NMDA; p > 0.05; two-tailed Student's tests). D. Melt curve of Wnt5a qPCR on control cells. The melt curve on NMDA-stimulated cells was similar (not shown). E. RT-PCR results of Wnt5a in control and NMDA-treated cells. Image collected and cropped by CiteAb from the following open publication (https://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Wnt-5a by Western Blot View Larger

Detection of Mouse Wnt-5a by Western Blot NMDAR-elicited Wnt5a increase requires translation but not transcription. A. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μΜ anisomycin for 30 min, and then incubated with 50 μΜ NMDA for 15 min, followed by Wnt5a immunoblotting. The Graph is a summary of three independent experiments (**, p < 0.01; One-way ANOVA). B. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 20 μΜ actinomycin D for 30 min, followed by addition of 50 μΜ NMDA for 15 min. Wnt5a protein was detected by Western blotting and quantified. The Graph is a summary of four independent experiments (*, p < 0.05; **, p < 0.01; One-way ANOVA). C. Primary cortical neurons (10 DIV) were treated with vehicle (Control) or 50 μΜ NMDA for 15 min. Wnt5a mRNA was quantified by Real-time RT-PCR (qPCR). The summary graph is from three independent experiments (40 cycles, CT values: 25.1 ± 0.5/control vs. 25.6 ± 0.3/NMDA; p > 0.05; two-tailed Student's tests). D. Melt curve of Wnt5a qPCR on control cells. The melt curve on NMDA-stimulated cells was similar (not shown). E. RT-PCR results of Wnt5a in control and NMDA-treated cells. Image collected and cropped by CiteAb from the following open publication (https://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Wnt-5a by Western Blot View Larger

Detection of Mouse Wnt-5a by Western Blot mTOR signaling pathway is not required for the NMDAR-dependent Wnt5a protein synthesis. Primary cortical neurons (10 DIV) were pre-treated with vehicle (Control) or 25nΜ Rapamycin for 30 min, followed by addition of 50 μΜ NMDA for 15 min. Western blotting analysis of Wnt5a and phosphor-P70S6K proteins were performed. Graphs (mean ± SEM) are from four independent experiments (*, p < 0.05; **, p < 0.01; #, p > 0.05; One-way ANOVA). Image collected and cropped by CiteAb from the following open publication (https://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-1), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Mouse Wnt-5a by Immunohistochemistry View Larger

Detection of Mouse Wnt-5a by Immunohistochemistry PGC depletion in Ror2 and Wnt5a mutants.(A–C) PGCs were visualized by wholemount SSEA1 immunostaining with SSEA1 antibody in e10.25 WT, Ror2, and Wnt5a embryos. (E–G) Gonadal ridges from e11.5 stained with GCNA, and (I–K) e12.5 male gonads stained with GCNA antibody. The caudal end is down in all images. (D, H, L) Quantification of PGCs in the entire e10.25 embryo, e11.5 and e12.5 gonads from confocal stacks, with individuals denoted as WT (diamond), Ror2Y324C mutants (triangle), and Wnt5a null (circle), and means indicated as bars. Consistent with appearances, a significant reduction of PGCs was observed in Wnt5a and from e11.5 onward in Ror2Y324C. We noted no significant difference in the number of PGCs between XX and XY gonads at e12.5 (not shown). Results of the Student's t-test are indicated, * p<0.05, **, p<0.01, ***p<0.001. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pgen.1002428), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-5a

Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23-24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1, 2).

Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mouse and human Wnt-5a share 97% amino acid identity.

References
  1. Miller, J.R. (2002) Genome Biol. 3:3001.
  2. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
  3. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
  4. Topol, L. et al. (2003) J. Cell Biol 162:899.
  5. Torres, M. et al. (1996) J. Cell Biol. 133:1123.
  6. Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
Long Name
Wingless-type MMTV Integration Site Family, Member 5a
Entrez Gene IDs
7474 (Human); 22418 (Mouse)
Alternate Names
hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein; Wnt5a; Wnt-5a

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Citations for Mouse/Rat Wnt-5a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

55 Citations: Showing 1 - 10
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  1. Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates
    Authors: MW Susman, EP Karuna, RC Kunz, TS Gujral, AV Cantú, SS Choi, BY Jong, K Okada, MK Scales, J Hum, LS Hu, MW Kirschner, R Nishinakam, S Yamada, DJ Laird, LE Jao, SP Gygi, ME Greenberg, HH Ho
    Elife, 2017-09-08;6(0):.
  2. Discrete somatic niches coordinate proliferation and migration of primordial germ cells via Wnt signaling
    J Cell Biol, 2016-07-11;214(2):215-29.
  3. Wnt5a Induces Catabolic Signaling and Matrix Metalloproteinase Production in Human Articular Chondrocytes
    Authors: G Huang, S Chubinskay, W Liao, RF Loeser
    Osteoarthr. Cartil., 2017-06-03;0(0):.
  4. Discordant Gene Expression Signatures and Related Phenotypic Differences in Lamin A-and A/C-Related Hutchinson-Gilford Progeria Syndrome (HGPS).
    Authors: Plasilova M, Chattopadhyay C, Ghosh A et al.
    PLoS One.
  5. Wnt5a is a TLR2/4-ligand that induces tolerance in human myeloid cells
    Authors: Chang-Graham AL, Danhof HA, Engevik MA et al.
    Commun Biol
  6. Wnt5a Promotes Axon Elongation in Coordination with the Wnt-Planar Cell Polarity Pathway
    Authors: Ahmad, S;Attisano, L;
    Cells
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  7. LGR5 Expression Predicting Poor Prognosis Is Negatively Correlated with WNT5A in Colon Cancer
    Authors: Mehdawi, LM;Ghatak, S;Chakraborty, P;Sjölander, A;Andersson, T;
    Cells
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. WNT5A inhibition alters the malignant peripheral nerve sheath tumor microenvironment and enhances tumor growth
    Authors: CS Thomson, J Pundavela, MR Perrino, RA Coover, K Choi, KE Chaney, TA Rizvi, DA Largaespad, N Ratner
    Oncogene, 2021-06-02;40(24):4229-4241.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Coordinated changes in the expression of Wnt pathway genes following human and rat peripheral nerve injury
    Authors: AC van Vliet, J Lee, M van der Po, MRJ Mason, JN Noordermee, LG Fradkin, MR Tannemaat, MJA Malessy, J Verhaagen, F De Winter
    PLoS ONE, 2021-04-13;16(4):e0249748.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  10. Wnt5a expression and prognosis in stage II-III colon cancer
    Authors: CM Lund, A Dyhl-Polk, DL Nielsen, LB Riis
    Transl Oncol, 2020-10-09;14(1):100892.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Wnt Signaling Pathway Dysregulation in the Aging Brain: Lessons From the Octodon degus
    Authors: Nibaldo C. Inestrosa, Cheril Tapia-Rojas, Carolina B. Lindsay, Juan Manuel Zolezzi
    Frontiers in Cell and Developmental Biology
  12. Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency
    Authors: W Kawarazaki, R Mizuno, M Nishimoto, N Ayuzawa, D Hirohama, K Ueda, F Kawakami-M, S Oba, T Marumo, T Fujita
    J. Clin. Invest., 2020-08-03;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  13. Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities
    Authors: S Ikezu, H Yeh, JC Delpech, ME Woodbury, AA Van Enoo, Z Ruan, S Sivakumara, Y You, C Holland, T Guillamon-, A Yoshii-Kit, MB Botros, C Madore, PH Chao, A Desani, S Manimaran, SV Kalavai, WE Johnson, O Butovsky, M Medalla, JI Luebke, T Ikezu
    Mol. Psychiatry, 2020-02-18;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Detection
  14. Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors
    Authors: X Wu, EM van Dijk, JP Ng-Blichfe, IST Bos, C Ciminieri, M Königshoff, LEM Kistemaker, R Gosens
    Cells, 2019-09-25;8(10):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. THE CANONICAL WNT PATHWAY IN GASTRIC CARCINOMA
    Authors: Levindo Alves de de OLIVEIRA, Celina Tizuko Fujiyama OSHIMA, Pedro Augusto SOFFNER, Marcelo de Souza SILVA, Rodrigo Rego LINS, Andréa Cristina de Moraes MALINVERNI et al.
    ABCD. Arquivos Brasileiros de Cirurgia Digestiva (São Paulo)
  16. Histopathologic and immunohistochemical findings in congenital anorectal malformations
    Authors: Hui Xiao, Rui Huang, Dai Xiao Cui, Ping Xiao, Mei Diao, Long Li
    Medicine (Baltimore)
  17. Wnt5a Contributes to the Differentiation of Human Embryonic Stem Cells into Lentoid Bodies Through the Noncanonical Wnt/JNK Signaling Pathway
    Authors: C Han, J Li, C Wang, H Ouyang, X Ding, Y Liu, S Chen, L Luo
    Invest. Ophthalmol. Vis. Sci., 2018-07-02;59(8):3449-3460.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Reduced Cdc42 Activity Compromises Hematopoiesis-Supportive Function Of Fanconi Anemia Mesenchymal Stromal Cells
    Authors: J Xu, X Li, A Cole, Z Sherman, W Du
    Stem Cells, 2018-02-09;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. ERAD-dependent control of the Wnt secretory factor Evi
    Authors: K Glaeser, M Urban, E Fenech, O Voloshanen, D Kranz, F Lari, JC Christians, M Boutros
    EMBO J., 2018-01-29;37(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice
    Authors: CM Chen, LL Orefice, SL Chiu, TA LeGates, S Hattar, RL Huganir, H Zhao, B Xu, R Kuruvilla
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-09;114(4):E619-E628.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
    Authors: Hoeke A Baarsma
    J. Exp. Med, 2016-12-15;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development
    Sci Rep, 2016-08-26;6(0):32094.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  23. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016-07-11;0(0):.
    Applications: Bioassay
  24. IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling
    PLoS ONE, 2016-05-23;11(5):e0156002.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  25. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells
    Authors: T Skaria, E Bachli, G Schoedon
    Cell Adh Migr, 2016-05-09;11(1):24-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  26. WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
    Authors: Bruce F Kimler
    J. Endocrinol., 2016-03-14;229(2):197-207.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. Thyroid hormone-regulated Wnt5a/Ror2 signaling is essential for dedifferentiation of larval epithelial cells into adult stem cells in the Xenopus laevis intestine.
    Authors: Ishizuya-Oka, Atsuko, Kajita, Mitsuko, Hasebe, Takashi
    PLoS ONE, 2014-09-11;9(9):e107611.
    Species: Xenopus
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Blocking, Western Blot
  28. Diabetic Osteopenia by Decreased beta -Catenin Signaling Is Partly Induced by Epigenetic Derepression of sFRP-4 Gene
    Authors: Kiyoshi Mori, Riko Kitazawa, Takeshi Kondo, Michiko Mori, Yasuhiro Hamada, Michiru Nishida et al.
    PLoS ONE
  29. WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
    Authors: Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K
    Mol Cancer, 2014-04-26;13(0):88.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  30. TGF-beta-activated kinase 1 (TAK1) signaling regulates TGF-beta-induced WNT-5A expression in airway smooth muscle cells via Sp1 and beta-catenin.
    Authors: Kumawat K, Menzen M, Slegtenhorst R, Halayko A, Schmidt M, Gosens R
    PLoS ONE, 2014-04-11;9(4):e94801.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.
    Authors: Dawes L, Sugiyama Y, Lovicu F, Harris C, Shelley E, McAvoy J
    Dev Biol, 2013-11-08;385(2):291-303.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  32. A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing.
    Authors: Florian M, Nattamai K, Dorr K, Marka G, Uberle B, Vas V, Eckl C, Andra I, Schiemann M, Oostendorp R, Scharffetter-Kochanek K, Kestler H, Zheng Y, Geiger H
    Nature, 2013-10-20;503(7476):392-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  33. Adenovirus-Mediated Wnt5a Expression Inhibits the Telogen-To-Anagen Transition of Hair Follicles in Mice
    Authors: Yi-Zhan Xing, Rui-Min Wang, Ke Yang, Hai-Ying Guo, Fang Deng, Yu-Hong Li et al.
    International Journal of Medical Sciences
  34. Wnt5a Induces a Tolerogenic Phenotype of Macrophages in Sepsis and Breast Cancer Patients
    Authors: Caroline Bergenfelz, Catharina Medrek, Elin Ekström, Karin Jirström, Helena Janols, Marlene Wullt et al.
    The Journal of Immunology
  35. Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
    J. Cell. Sci., 2012-02-10;125(0):2017-29.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  36. NMDA receptor activation stimulates transcription-independent rapid wnt5a protein synthesis via the MAPK signaling pathway.
    Mol Brain, 2012-01-04;5(0):1.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  37. Separate and distinctive roles for Wnt5a in tongue, lingual tissue and taste papilla development
    Authors: Hong-Xiang Liu, Ann S. Grosse, Ken Iwatsuki, Yuji Mishina, Deborah L. Gumucio, Charlotte M. Mistretta
    Developmental Biology
  38. Ror2 Enhances Polarity and Directional Migration of Primordial Germ Cells
    Authors: Diana J. Laird, Svetlana Altshuler-Keylin, Michael D. Kissner, Xin Zhou, Kathryn V. Anderson
    PLoS Genetics
  39. WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity.
    Authors: Li B, Zhong L, Yang X, Andersson T, Huang M, Tang SJ
    PLoS ONE, 2011-08-17;6(8):e22920.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  40. Wnt5a suppresses epithelial ovarian cancer by promoting cellular senescence.
    Authors: Bitler BG, Nicodemus JP, Li H, Cai Q, Wu H, Hua X, Li T, Birrer MJ, Godwin AK, Cairns P, Zhang R
    Cancer Res., 2011-08-04;71(19):6184-94.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  41. Effects of Wnt5a protein on proliferation and apoptosis in JAR choriocarcinoma cells
    Authors: Sha Peng, Junlin Zhang, Jiahuan Chen, Huayan Wang
    Molecular Medicine Reports
  42. A Novel Role for Wnt/Ca2+ Signaling in Actin Cytoskeleton Remodeling and Cell Motility in Prostate Cancer
    Authors: Qin Wang, Andrew J. Symes, Corrina A. Kane, Alex Freeman, Joseph Nariculam, Philippa Munson et al.
    PLoS ONE
  43. Evidence for altered Wnt signaling in psoriatic skin.
    Authors: Gudjonsson JE, Johnston A, Stoll SW
    J. Invest. Dermatol., 2010-04-08;130(7):1849-59.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Bioassay, IHC-P
  44. Wnt5a mediates nerve growth factor-dependent axonal branching and growth in developing sympathetic neurons.
    Authors: Bodmer D, Levine-Wilkinson S, Richmond A, Hirsh S, Kuruvilla R
    J. Neurosci., 2009-06-10;29(23):7569-81.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  45. Wnt5a is expressed in murine and human atherosclerotic lesions.
    Authors: Christman MA, Goetz DJ, Dickerson E, McCall KD, Lewis CJ, Benencia F, Silver MJ, Kohn LD, Malgor R
    Am. J. Physiol. Heart Circ. Physiol., 2008-05-02;294(6):H2864-70.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  46. Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10.
    Authors: Pereira C, Schaer DJ, Bachli EB, Kurrer MO, Schoedon G
    Arterioscler. Thromb. Vasc. Biol., 2008-01-03;28(3):504-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  47. Dickkopf-1 is a master regulator of joint remodeling.
    Authors: Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
    Nat. Med., 2007-01-21;13(2):156-63.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  48. Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
    Authors: Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J
    Mol. Biol. Cell, 2006-10-11;17(12):5163-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  49. The Wingless homolog WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation.
    Authors: Blumenthal A, Ehlers S, Lauber J, Buer J, Lange C, Goldmann T, Heine H, Brandt E, Reiling N
    Blood, 2006-04-06;108(3):965-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  50. Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion.
    Authors: Castelo-Branco G, Sousa KM, Bryja V, Pinto L, Wagner J, Arenas E
    Mol. Cell. Neurosci., 2005-10-21;31(2):251-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  51. Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration.
    Authors: Polesskaya A, Seale P, Rudnicki MA
    Cell, 2003-06-27;113(7):841-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  52. Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells in vivo.
    Authors: Murdoch B, Chadwick K, Martin M, Shojaei F, Shah KV, Gallacher L, Moon RT, Bhatia M
    Proc. Natl. Acad. Sci. U.S.A., 2003-03-07;100(6):3422-7.
    Species: Xenopus
    Sample Types: Cell Culture Supernates
    Applications: Immunodepletion
  53. Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP.
    Authors: Ahumada A, Slusarski DC, Liu X, Moon RT, Malbon CC, Wang HY
    Science, 2002-12-06;298(5600):2006-10.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Immunodepletion
  54. Single-cell RNA sequencing reveals dysregulated fibroblast subclusters in prurigo nodularis
    Authors: JR Patel, MZ Joel, KK Lee, A Kambala, H Cornman, O Oladipo, M Taylor, J Deng, V Parthasara, K Cravero, M Marani, R Zhao, S Sankararam, R Li, T Pritchard, V Rebecca, MM Kwatra, W Jin Ho, X Dong, S Kang, SG Kwatra
    bioRxiv : the preprint server for biology, 2023-02-03;0(0):.
  55. Regulation of lipid synthesis by the RNA helicase Mov10 controls Wnt5a production.
    Authors: W Wang, N Snyder, A J Worth et al.
    Oncogenesis

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Mouse/Rat Wnt-5a Antibody
By Anonymous on 02/19/2018
Application: WB Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse

Non-specific bands at 64kDa, actual band at 49KDa. Recommend to use 7.5% gel for better separation.

Western Blot Mouse/Rat Wnt-5a Antibody AF645