Mouse/Rat TNF RI/TNFRSF1A Antibody

Catalog #: MAB425
9 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB425
MAB425-SP

Detection of Mouse and Rat TNF RI/TNFRSF1A by Western Blot.

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Product Details

Citations (9)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat TNF RI/TNFRSF1A Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse TNF RI in ELISAs and Western blots. In ELISAs, no cross-reactivity was observed with recombinant human (rh) TNF RI, rhTNF RII, recombinant mouse TNF RII, rhTNF-alpha, recombinant rat TNF-alpha, recombinant porcine TNF-alpha, or rhTNF-beta.

Source

Monoclonal Rat IgG₁ Clone # 47803

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse TNF RI
Ile22-Ala212
Accession # P25118

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Mouse TNF RI/TNFRSF1A Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse TNF RI/TNFRSF1A Biotinylated Antibody (Catalog # BAF425)

Standard: Recombinant Mouse sTNF RI/TNFRSF1A Protein (Catalog # 425-R1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Rat TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of C6 rat glioma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB425) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF RI functions as a receptor for TNF-alpha and TNF-beta. It is a member of the TNF receptor superfamily and is designated TNFRSF1A.

Long Name

Tumor Necrosis Factor Receptor I

Entrez Gene IDs

7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)

Alternate Names

CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse/Rat TNF RI/TNFRSF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Spinal TNF-? receptor 1 is differentially required for phrenic long-term facilitation (pLTF) over the course of motor neuron death in adult rats
Authors: Lewis, RD;Keilholz, AN;Smith, CL;Burd, EA;Nichols, NL;
Frontiers in physiology
Species: Rat
Sample Types: In Vivo
Applications: In vivo assay
The death-inducing activity of RIPK1 is regulated by the pH environment
Authors: Kenta Moriwaki, Sakthi Balaji, Francis Ka-Ming Ka-Ming Chan
Science Signaling
Berberine ameliorates lipopolysaccharide?induced inflammatory responses in mouse inner medullary collecting duct?3 cells by downregulation of NF?kappaB pathway
Authors: DG Kim, JW Choi, IJ Jo, MJ Kim, HS Lee, SH Hong, HJ Song, GS Bae, SJ Park
Mol Med Rep, 2019-11-19;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
FKBP12 mediates necroptosis by initiating RIPK1/RIPK3/MLKL signal transduction in response to TNF receptor 1 ligation
Authors: Z Wang, J Feng, J Yu, G Chen
J. Cell. Sci., 2019-05-20;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF Receptor 1 Domain Antibody Formatted for In Vivo Half-Life Extension.
Authors: Goodall L, Ovecka M, Rycroft D, Friel S, Sanderson A, Mistry P, Davies M, Stoop A
PLoS ONE, 2015-09-09;10(9):e0137065.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Capture)
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012-01-25;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
J. Immunol., 2006-08-01;177(3):1967-74.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development
Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005-07-01;302(1):172-81.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development
Selective macrophage suppression during sepsis.
Authors: Ellaban E, Bolgos G, Remick D
Cell. Immunol., 2005-02-26;231(1):103-11.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development