Mouse/Rat SCF/c-kit Ligand Antibody Summary
Gln26-Ala189
Accession # P21581
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse and Rat SCF/c‑kit Ligand by Western Blot. Western blot shows lysates of rat adipose tissue, rat liver tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6998) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SCF/c-kit Ligand at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCF/c-kit Ligand
SCF (Stem Cell Factor; also kit ligand and MGF) is a 40-45 kDa member of the SCF family of molecules. It is expressed by multiple cell types, including Sertoli cells, neurons, select fibroblasts, mast cells, melanocytes and dendritic cells. SCF binds to SCFR/c-kit (for kitten; the animal used to isolate it), and occurs in both a soluble and membrane-bound form. The soluble form of SCF produces a transient activation of SCFR, while the membrane-bound form generates a long-lasting signal in the SCFR due to the ligation and retention of SCFR at the expressing cell surface. Mature rat SCF is a 248 amino acid (aa) type I transmembrane (TM) glycoprotein (aa 26-273). It contains a 189 aa extracellular region (aa 26-214) plus a 36 aa cytoplasmic domain (aa 238-273). Proteolytic cleavage after Ala190 generates a 25-38 kDa soluble form that circulates as both a monomer and noncovalent homodimer. There is also possible cleavage after Phe183 that will create a similarly-sized isoform. An alternative splice form also exists that contains a Gly substitution for aa 174-292. This 28-32 kDa TM form may also undergo cleavage to generate a soluble 19-21 kDa isoform. Over aa 26-189, rat SCF shares 94%, 77% and 79% aa sequence identity with mouse, feline and human SCF, respectively.
Product Datasheets
Citation for Mouse/Rat SCF/c-kit Ligand Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Gastric Electrical Pacing Reduces Apoptosis of Interstitial Cells of Cajal via Antioxidative Stress Effect Attributing to Phenotypic Polarization of M2 Macrophages in Diabetic Rats
Authors: H Wang, K Zhao, Y Ba, T Gao, N Shi, Q Niu, C Liu, Y Chen
Oxidative Medicine and Cellular Longevity, 2021-02-28;2021(0):1298657.
Species: Rat
Sample Types: Tissue Homogenates
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