Mouse/Rat PDGF-BB Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 22 | 24 | 24 |
Mean (pg/mL) | 49.7 | 252 | 823 | 52.1 | 246 | 811 |
Standard Deviation | 4.5 | 18.7 | 36.7 | 5.3 | 21.1 | 40.4 |
CV% | 9.1 | 7.4 | 4.5 | 10.2 | 8.6 | 5 |
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 22 | 22 | 23 |
Mean (pg/mL) | 61.1 | 298 | 1019 | 69.5 | 305 | 1048 |
Standard Deviation | 5.6 | 24.6 | 40.3 | 6.6 | 26.3 | 98.5 |
CV% | 9.2 | 8.3 | 4 | 9.5 | 8.6 | 9.4 |
Recovery
The recovery of PDGF-BB spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Mouse Cell Culture Supernates (n=8) | 100 | 89-118 |
Mouse Heparin Plasma (n=5) | 96 | 85-113 |
Rat Cell Culture Supernates (n=8) | 102 | 94-114 |
Rat EDTA Plasma (n=5) | 102 | 86-111 |
Rat Heparin Plasma (n=5) | 102 | 85-111 |
Rat Serum (n=5) | 100 | 89-113 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: PDGF-BB
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
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Citations for Mouse/Rat PDGF-BB Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
22
Citations: Showing 1 - 10
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Aortic Fibrosis in Insulin-Sensitive Mice with Endothelial Cell-Specific Deletion of Ceacam1 Gene
Authors: R Abu Helal, HT Muturi, AD Lee, W Li, HE Ghadieh, SM Najjar
International Journal of Molecular Sciences, 2022-04-14;23(8):.
Species: Mouse
Sample Types: Plasma
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Pharmacological Inhibition of Glutaminase 1 Attenuates Alkali-Induced Corneal Neovascularization by Modulating Macrophages
Authors: Y Feng, X Yang, J Huang, M Shen, L Wang, X Chen, Y Yuan, C Dong, X Ma, F Yuan
Oxidative Medicine and Cellular Longevity, 2022-03-19;2022(0):1106313.
Species: Mouse
Sample Types: Protein
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A Switch from Cell-Associated to Soluble PDGF-B Protects against Atherosclerosis, despite Driving Extramedullary Hematopoiesis
Authors: RJHA Tillie, TL Theelen, K van Kuijk, L Temmerman, J de Bruijn, M Gijbels, C Betsholtz, EAL Biessen, JC Sluimer
Cells, 2021-07-10;10(7):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Probiotic-Derived Polyphosphate Accelerates Intestinal Epithelia Wound Healing through Inducing Platelet-Derived Mediators
Authors: S Isozaki, H Konishi, M Fujiya, H Tanaka, Y Murakami, S Kashima, K Ando, N Ueno, K Moriichi, T Okumura
Mediators of Inflammation, 2021-03-29;2021(0):5582943.
Species: Rat
Sample Types: Plasma
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Sulfated polysaccharide directs therapeutic angiogenesis via endogenous VEGF secretion of macrophages
Authors: Y Yu, K Dai, Z Gao, W Tang, T Shen, Y Yuan, J Wang, C Liu
Science Advances, 2021-02-10;7(7):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting apoptosis
Authors: W El Bakly, M Medhat, M Shafei, R Tash, M Elrefai, Y Shoukry, NN Omar
Heliyon, 2020-09-21;6(9):e05006.
Species: Rat
Sample Types: Plasma
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Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors
Authors: K Hosaka, Y Yang, T Seki, Q Du, X Jing, X He, J Wu, Y Zhang, H Morikawa, M Nakamura, M Scherzer, X Sun, Y Xu, T Cheng, X Li, X Liu, Q Li, Y Liu, A Hong, Y Chen, Y Cao
Nat Commun, 2020-07-24;11(1):3704.
Species: Mouse
Sample Types: Cell Culture Supernates
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Transplantation of Endothelial Progenitor Cells in Obese Diabetic Rats Following Myocardial Infarction: Role of Thymosin Beta-4
Authors: KK Poh, PSS Lee, AH Djohan, MJ Galupo, GG Songco, TC Yeo, HC Tan, AM Richards, L Ye
Cells, 2020-04-12;9(4):.
Species: Rat
Sample Types: Cell Culture Supernates
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?-catenin-activated autocrine PDGF/Src signaling is a therapeutic target in pancreatic cancer
Authors: TL Kuo, KH Cheng, YS Shan, LT Chen, WC Hung
Theranostics, 2019-01-01;9(2):324-336.
Species: Mouse
Sample Types: Serum
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Circuit Design Features of a Stable Two-Cell System
Authors: X Zhou, RA Franklin, M Adler, JB Jacox, W Bailis, JA Shyer, RA Flavell, A Mayo, U Alon, R Medzhitov
Cell, 2018-02-01;172(4):744-757.e17.
Species: Mouse
Sample Types: Cell Culture Supernates
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Rosmarinic acid attenuates hepatic fibrogenesis via suppression of hepatic stellate cell activation/proliferation and induction of apoptosis
Authors: NM El-Lakkany, WH El-Maadawy, SH Seif El-Di, OA Hammam, SH Mohamed, SM Ezzat, MM Safar, S Saleh
Asian Pac J Trop Med, 2017-05-17;10(5):444-453.
Species: Rat
Sample Types: Tissue Homogenates
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Freeze-Dried Platelet-Rich Plasma Accelerates Bone Union with Adequate Rigidity in Posterolateral Lumbar Fusion Surgery Model in Rats
Sci Rep, 2016-11-11;6(0):36715.
Species: Rat
Sample Types: Plasma
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Integration of growth factor gene delivery with collagen-triggered wound repair cascades using collagen-mimetic peptides
Authors: Morgan A Urello
Bioeng Transl Med, 2016-10-19;1(2):207-219.
Species: Mouse
Sample Types: Complex Sample Type
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Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts
Authors: Koji Ueno
Sci Rep, 2016-06-22;6(0):28538.
Species: Mouse
Sample Types: Cell Culture Supernates
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The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages
Nat Commun, 2016-05-06;7(0):11385.
Species: Mouse
Sample Types: Cell Lysates
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Uncoupling between inflammatory and fibrotic responses to silica: evidence from MyD88 knockout mice.
Authors: Re S, Giordano G, Yakoub Y, Devosse R, Uwambayinema F, Couillin I, Ryffel B, Marbaix E, Lison D, Huaux F
PLoS ONE, 2014-07-22;9(7):e99383.
Species: Mouse
Sample Types: BALF
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Pulsed estrogen therapy prevents post-OVX porcine dura mater microvascular network weakening via a PDGF-BB-dependent mechanism.
Authors: Glinskii, Olga V, Huxley, Virginia, Glinskii, Vladimir, Rubin, Leona J, Glinsky, Vladisla
PLoS ONE, 2013-12-09;8(12):e82900.
Species: Mouse
Sample Types: Cell Lysates
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Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease.
Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y
J. Clin. Invest., 2011-10-17;121(11):4548-66.
Species: Mouse
Sample Types: Tissue Homogenates
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Statins restore ischemic limb blood flow in diabetic microangiopathy via eNOS/NO upregulation but not via PDGF-BB expression.
Authors: Fujii T, Onimaru M, Yonemitsu Y, Kuwano H, Sueishi K
Am. J. Physiol. Heart Circ. Physiol., 2008-04-25;294(6):H2785-91.
Species: Mouse
Sample Types: Tissue Homogenates
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The role of the receptor for advanced glycation end-products in lung fibrosis.
Authors: He M, Kubo H, Ishizawa K, Hegab AE, Yamamoto Y, Yamamoto H, Yamaya M
Am. J. Physiol. Lung Cell Mol. Physiol., 2007-10-19;293(6):L1427-36.
Species: Mouse
Sample Types: BALF
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Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
Proc. Natl. Acad. Sci. U.S.A., 2007-10-17;104(43):17069-74.
Species: Mouse
Sample Types: Plasma
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Pharmacological inhibition of epsilon PKC suppresses chronic inflammation in murine cardiac transplantation model.
Authors: Koyanagi T, Noguchi K, Ootani A, Inagaki K, Robbins RC, Mochly-Rosen D
J. Mol. Cell. Cardiol., 2007-06-21;43(4):517-22.
Species: Mouse
Sample Types: Tissue Homogenates
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