Mouse/Rat CCR8 Antibody

Catalog #: AF5997 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5997
AF5997-SP

Detection of Mouse and Rat CCR8 by Western Blot.

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Mouse/Rat CCR8 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat CCR8 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant mouse (rm) CCR1, rmCCR4, and rmCCR5 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CCR8
Met1-Met33, Asn92-Lys105, Tyr170-Ile200, His262-Leu278
Accession # P56484

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Rat CCR8 by Western Blot. Western blot shows lysates of mouse thymus tissue and rat thymus tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse/Rat CCR8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5997) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR8 at approximately 41-43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR8

CCR8 (C-C chemokine receptor 8; also CD198) is a 41‑43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8⁺ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Mouse CCR8 is a 7-transmembrane protein that is 353 amino acids (aa) in length. It contains a 33 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. In mouse, CCR8 is N- and possibly O‑glycosylated, and known to be sulfated on Tyr14 and 15. The unusual nature of these posttranslational modifications may lead to anomalous migration in SDS-PAGE. Over aa sequences 1‑33 and 92‑105 collectively, mouse CCR8 shares 64% and 85% aa identity with human and rat CCR8, respectively.

Entrez Gene IDs

1237 (Human); 12776 (Mouse)

Alternate Names

CC chemokine receptor 8; C-C CKR-8; CC-chemokine receptor chemr1; CC-CKR-8; CCR8; CCR-8; CD198; CDw198 antigen; CDw198; chemokine (C-C motif) receptor 8; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; Chemokine receptor-like 1; ChemR1; CKRL1; CKR-L1; CKRL1MGC129966; CMKBR8MGC129973; CMKBRL2C-C chemokine receptor type 8; CY6; GPRCL1; GPRCY6; GPR-CY6; TER-1; TER1CC chemokine receptor CHEMR1