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Mouse Rae-1 alpha / beta /δ/ epsilon Antibody

Catalog #: MAB17581
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB17581
MAB17581-SP
Product Details
Citations (4)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Rae-1 alpha / beta /δ/ epsilon Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Rae‑1 alpha / beta /δ/ epsilon. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody strongly recognizes Rae-1 beta and Rae-1δ. It shows weaker staining of Rae-1 alpha and Rae-1 epsilon transfectants. It does not recognize Rae-1 gamma.
Source
Monoclonal Rat IgG2A Clone # 199205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with Rae-1 beta (Accession # NP_033043) and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ Leu29-Ser227 (Accession # Q9JI58)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-6 ng/mL of this antibody will block 50% of the binding of 300 ng/mL of Biotinylated Recombinant Mouse NKG2D Fc Chimera to immobilized Recombinant Mouse Rae-1δ Fc Chimera (Catalog # 1134‑RA) coated at 1 µg/mL (100 µL/well). At 100 ng/mL, this antibody will block >90% of the binding.

Blocking of Recombinant Mouse Rae-1 beta is observed at a similar antibody concentration, however, this antibody does not block the binding of Recombinant Mouse NKG2D Fc Chimera to Recombinant Mouse Rae-1 alpha or Rae-1 epsilon in this assay.

 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rae-1

Rae-1 alpha, beta, gamma, δ and epsilon (retinoic acid early transcript 1) comprise a family of closely related (88‑95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Long Name
Retinoic Acid Early Transcript 1
Entrez Gene IDs
8480 (Human); 19368 (Mouse)
Alternate Names
dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1; Rae1; Rae-1

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Citations for Mouse Rae-1 alpha / beta /δ/ epsilon Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Attribution of NKG2DL to the inhibition of early stage allogeneic tumors in mice
    Authors: Li Hua, Mingli Fang, Boqi Dong, Sheng Guo, Cuiyun Cui, Jiwei Liu et al.
    Oncotarget
  2. Ras activation induces expression of Raet1 family NK receptor ligands.
    J. Immunol., 2012-07-13;189(4):1826-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair.
    Authors: Yoshida S, Mohamed RH, Kajikawa M, Koizumi J, Tanaka M, Fugo K, Otsuka N, Maenaka K, Yagita H, Chiba H, Kasahara M
    J. Immunol., 2012-03-07;188(8):3972-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
    Authors: Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL
    PLoS Pathog., 2012-01-12;8(1):e1002481.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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