Mouse PlGF-2 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 58 | 136 | 470 | 63 | 155 | 493 |
Standard Deviation | 4.6 | 7.6 | 14 | 6 | 15 | 43 |
CV% | 7.9 | 5.6 | 3 | 9.5 | 9.7 | 8.7 |
Recovery
The recovery of mouse PlGF-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Supernates (n=6) | 97 | 84-119 |
EDTA Plasma (n=6) | 94 | 88-104 |
Serum (n=6) | 90 | 81-102 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: PlGF-2
Placenta growth factor (PlGF) is a dimeric growth factor in the PDGF/VEGF family. Alternative splicing generates at least three human PlGF isoforms PlGF 1, PlGF 2, and PlGF 3. PlGF 2 is the only isoform that contains a heparin binding region. It is expressed by villous trophoblasts, decidual cells, erythroblasts, keratinocytes, and some endothelial cells. PlGF competes with VEGF for binding and activating VEGF R1/Flt 1. This results in increased angiogenesis by making more VEGF available for activation of VEGF R2. PlGF 2 also exhibits heparin dependent binding of Neuropilins-1 and 2 which serve as VEGF co-receptors. It can inhibit nerve growth cone collapse and induce monocyte production of inflammatory cytokines including VEGF.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
![](http://resources.rndsystems.com/images/site/wells-plain.png)
![](http://resources.rndsystems.com/images/site/wells-plain.png)
![](http://resources.rndsystems.com/images/site/wells-plain.png)
![](http://resources.rndsystems.com/images/site/wells-blue.png)
![](http://resources.rndsystems.com/images/site/wells-yellow.png)
Citations for Mouse PlGF-2 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
17
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Human umbilical cord mesenchymal stem cell-derived extracellular vesicles loaded with TFCP2 activate Wnt/?-catenin signaling to alleviate preeclampsia
Authors: Yang, Z;Jia, X;Deng, Q;Luo, M;Hou, Y;Yue, J;Mei, J;Shan, N;Wu, Z;
International immunopharmacology
Species: Mouse
Sample Types: Serum
-
Neutralization of the induced VEGF-A potentiates the therapeutic effect of an anti-VEGFR2 antibody on gastric cancer in vivo
Authors: T Mashima, T Wakatsuki, N Kawata, MK Jang, A Nagamori, H Yoshida, K Nakamura, T Migita, H Seimiya, K Yamaguchi
Scientific Reports, 2021-07-23;11(1):15125.
Species: Mouse
Sample Types: Plasma
-
Acid Sensing Ion Channel 2a Is Reduced in the Reduced Uterine Perfusion Pressure Mouse Model and Increases Seizure Susceptibility in Pregnant Mice
Authors: M Jones-Muha, Q Shao, L Cain-Shiel, JP Shaffery, JP Warrington
Cells, 2021-05-08;10(5):.
Species: Mouse
Sample Types: Serum
-
High-fat diet from parental generation exaggerates body and adipose tissue weights in pregnant offspring
Authors: FT Spradley
PLoS ONE, 2020-08-20;15(8):e0237708.
Species: Rat
Sample Types: Plasma
-
Adrenergic receptor blockade attenuates placental ischemia-induced hypertension
Authors: FT Spradley, Y Ge, BP Haynes, JP Granger, CD Anderson
Physiol Rep, 2018-09-01;6(17):e13814.
Species: Rat
Sample Types: Plasma
-
Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
Mol. Cancer Ther., 2018-04-25;0(0):.
Species: Mouse
Sample Types: Plasma
-
Induction of hemangiosarcoma in mice after chronic treatment with S1P-modulator siponimod and its lack of relevance to rat and human
Authors: F Pognan, JA Mahl, M Papoutsi, D Ledieu, M Raccuglia, D Theil, SB Voytek, PJ Devine, K Kubek-Luck, N Claudio, A Cordier, A Heier, C Kolly, A Hartmann, SD Chibout, P Bouchard, C Trendelenb
Arch. Toxicol., 2018-03-19;0(0):.
Species: Mouse, Rat
Sample Types: Cell Culture Supernates, Plasma
-
Blockade of placental growth factor reduces vaso-occlusive complications in murine models of sickle cell disease
Authors: JM Gu, S Yuan, D Sim, K Abe, P Liu, M Rosenbruch, P Bringmann, K Kauser
Exp. Hematol., 2018-01-11;0(0):.
Species: Mouse
Sample Types: Plasma
-
Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence.
Authors: Oladipupo SS, Hu S, Santeford AC, Yao J, Kovalski JR, Shohet RV, Maslov K, Wang LV, Arbeit JM
Blood, 2011-02-09;117(15):4142-53.
Species: Human, Mouse
Sample Types: Plasma, Tissue Homogenates
-
Placental growth factor mediates aldosterone-dependent vascular injury in mice.
Authors: Jaffe IZ, Newfell BG, Aronovitz M
J. Clin. Invest., 2010-11-01;120(11):3891-900.
Species: Mouse
Sample Types: Tissue Homogenates
-
Inhibitory effect of atractylenolide I on angiogenesis in chronic inflammation in vivo and in vitro.
Authors: Wang C, Duan H, He L
Eur. J. Pharmacol., 2009-04-06;612(1):143-52.
Species: Mouse
Sample Types: Air Pouch Exudate
-
alpha2beta1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner.
Authors: Zhang Z, Ramirez NE, Yankeelov TE, Li Z, Ford LE, Qi Y, Pozzi A, Zutter MM
Blood, 2007-11-27;111(4):1980-8.
Species: Mouse
Sample Types: Cell Culture Supernates
-
Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
Proc. Natl. Acad. Sci. U.S.A., 2007-10-17;104(43):17069-74.
Species: Mouse
Sample Types: Plasma
-
Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
Am. J. Pathol., 2006-10-01;169(4):1167-82.
Species: Mouse
Sample Types: Tissue Homogenates
-
Impaired adipose tissue development in mice with inactivation of placental growth factor function.
Authors: Lijnen HR, Christiaens V, Scroyen I, Voros G, Tjwa M, Carmeliet P, Collen D
Diabetes, 2006-10-01;55(10):2698-704.
Species: Mouse
Sample Types: Tissue Homogenates
-
The human herpes virus 8-encoded chemokine receptor is required for angioproliferation in a murine model of Kaposi's sarcoma.
Authors: Jensen KK, Manfra DJ, Grisotto MG, Martin AP, Vassileva G, Kelley K, Schwartz TW, Lira SA
J. Immunol., 2005-03-15;174(6):3686-94.
Species: Mouse
Sample Types: Tissue Homogenates
-
Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation.
Authors: Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP
J. Immunol., 2005-01-01;174(1):215-22.
Species: Mouse
Sample Types: Serum
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsReviews for Mouse PlGF-2 Quantikine ELISA Kit
Average Rating: 5 (Based on 1 Review)
Have you used Mouse PlGF-2 Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by: