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Mouse Osteopontin/OPN Antibody

Catalog #: AF808
207 Citations 11 Reviews Datasheet / COA / SDS
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AF808
AF808-SP
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Osteopontin/OPN in C2C12 Mouse Cell Line and Mouse Splenocytes.
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Citations (207)
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Reviews (11)
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Mouse Osteopontin/OPN Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Osteopontin (OPN) in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteopontin/OPN (R&D Systems, Catalog # 441-OP)
Leu17-Asn294 (Glu99Gly)
Accession # Q547B5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.30 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Osteopontin/OPN (Catalog # 441-OP)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Mouse Osteopontin/OPN Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Osteopontin/OPN Biotinylated Antibody (Catalog # BAF808)

Standard: Recombinant Mouse Osteopontin/OPN Protein (Catalog # 441-OP)

Neutralization
Measured by its ability to neutralize Osteopontin/OPN-mediated adhesion of the HEK293 human embryonic kidney cell line. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 2 µg/mL Recombinant Mouse Osteopontin/OPN.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Osteopontin/OPN antibody in C2C12 Mouse Cell Line and Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

Osteopontin/OPN in C2C12 Mouse Cell Line and Mouse Splenocytes. Osteopontin/OPN was detected in immersion fixed C2C12 mouse myoblast cell line (left panel, positive stain) and mouse splenocytes (right panel, negative stain) using Goat Anti-Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and secreted molecule. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Osteopontin/OPN antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

Osteopontin/OPN in Mouse Thymus. Osteopontin/OPN was detected in perfusion fixed frozen sections of mouse thymus using Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Neutralization Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Mouse Osteopontin/OPN Antibody. View Larger

Cell Adhesion Mediated by Osteopontin/OPN and Neutralization by Mouse Osteopontin/OPN Antibody. Recombinant Mouse Osteopontin/OPN (441-OP), immobilized onto a microplate, supports the adhesion of the HEK293 human embryonic kidney cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Osteopontin/OPN (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Osteopontin/OPN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF808). The ND50 is typically 1-3 µg/mL.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence Notch ligands Jag1 and Dll1 are both required for segregation of hepatocytic fate centrally and biliary fate peripherally.(A) Immunolabeling for OPN and HNF4A of BMEL cells presented with DLL4 on 30 kPa substrates. Control cells were transduced with an shRNA vector coding for a non-mammalian target. shJag1 and shDll1 cells were transduced with shRNA vectors targeting Jag1 and Dll1, respectively. Scale bar is 150 µm. (B) Confocal imaging of immunolabeled SOX9 and HNF4A in control, shJag1, and shDll1 cells presented with DLL4 on 30 kPa substrates. Scale bar is 75 µm. (C) Quantification of OPN+ cell counts of control, shJag1, and shDll1 cells presented with DLL4 on 30 kPa substrates. (D) Quantification of SOX9 and HNF4A intensity of control, shJag1, and shDll1 cells presented with DLL4 on 30 kPa substrates. (C, D) Mean ± 95% CI.10.7554/eLife.38536.024Figure 7—source data 1.Summary table for OPN data in Figure 7C.10.7554/eLife.38536.025Figure 7—source data 2.Summary table for SOX9 and HNF4A data in Figure 7D.Summary table for OPN data in Figure 7C.Summary table for SOX9 and HNF4A data in Figure 7D.Regression analysis of OPN+ cell counts.Data in Figure 7B were separated into peripheral and central subsets for which dimensionless radius was greater than 0.75 (R>0.75) and less than 0.75 (R<0.75). Separate multiple regression models were generated for each data subset for which coefficient estimates (corresponding to mean change in cell counts) and 95% CI were plotted. For each factor, 95% CI that do not intersect with the dashed line indicate regression coefficient estimates for which P<0.05. Control, shJag1, and shDll1 cells were presented with DLL4 on 30 kPa substrates. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30589410), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Osteopontin/OPN by Western Blot View Larger

Detection of Mouse Osteopontin/OPN by Western Blot Altered expression of ECM proteins in Cyp1b1-/- LSEC.Western blot analysis of ECM proteins in the conditioned medium (CM) and cell lysates from LSEC was performed. (A) The levels of SPARC, Tenascin-C, TSP2, and Osteopontin were below the level of detection. Cyp1b1 LSEC produced TSP1, periostin, and fibronectin. (B) The quantitative assessment of the data. TSP1 level was significantly decreased in lysates from Cyp1b1-/- LSEC (***P< 0.001; n = 3), while periostin level was increased in lysates from Cyp1b1-/- LSEC. The level of fibronectin secreted into the conditioned medium was decreased in Cyp1b1-/- LSEC. These experiments were repeated with two isolation of LSEC with similar results. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30372497), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence Peripheral biliary differentiation is dependent on both Notch signaling and substrate stiffness.(A) Immunolabeling for OPN of BMEL cells presented with DLL4 on 30 kPa and 4 kPa substrates. Cells were treated with vehicle control (DMSO) or an inhibitor of Notch signaling ( gamma -secretase inhibitor X, GSI, 5 µM). (B) Quantification of OPN+ cell counts on 30 kPa and 4 kPa substrates after treatment with DMSO or GSI. (C) Immunolabeling for SOX9 and HNF4A of BMEL cells on 30 kPa and 4 kPa substrates. (D) Quantification of SOX9 and HNF4A intensity on 30 kPa and 4 kPa substrates. (E) RNA in situ hybridization for Jag1, Dll1, and Notch2 on 30 kPa and 4 kPa substrates. Cells were exogenously presented with IgG or DLL4. (A, C, E) Scale bars indicate 150 µm. (B, D) Mean ± 95% CI.10.7554/eLife.38536.013Figure 2—source data 1.Summary table for OPN data in Figure 2B and Figure 2—figure supplement 1.10.7554/eLife.38536.014Figure 2—source data 2.Summary table for SOX9 and HNF4A data in Figure 2D.Summary table for OPN data in Figure 2B and Figure 2—figure supplement 1.Summary table for SOX9 and HNF4A data in Figure 2D.Quantification of OPN+ cell counts in arrayed patterns.Cells were cultured on 30 kPa and 4 kPa substrates and presented with IgG, DLL1, DLL4, and JAG1. Treatments included vehicle control (DMSO) or an inhibitor of Notch signaling ( gamma -secretase inhibitor X, GSI, 5 µM).Regression analysis of OPN+ and ALB+ cell counts.Data in Figure 2B were separated into peripheral and central subsets for which dimensionless radius was greater than 0.75 (R>0.75) and less than 0.75 (R<0.75). Separate multiple regression models were generated for each data subset for which coefficient estimates (corresponding to mean change in cell counts) and 95% CI were plotted for OPN+ (A) and ALB+ (B) cells. For each factor, 95% CI that do not intersect with the dashed line indicate regression coefficient estimates for which P<0.05. Cells were cultured on 30 kPa and 4 kPa substrates and presented with IgG, DLL1, DLL4, and JAG1. Treatments included vehicle control (DMSO) or an inhibitor of Notch signaling ( gamma -secretase inhibitor X, GSI, 5 µM).Quantification of ALB+ cell counts in arrayed patterns.Cells were cultured on 30 kPa substrates and presented with IgG, DLL1, DLL4, and JAG1. Treatments included vehicle control (DMSO) or an inhibitor of Notch signaling ( gamma -secretase inhibitor X, GSI, 5 µM). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30589410), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence Characterisation of mouse liver progenitor cell lines.(A) Mouse liver progenitor cell (LPC) lines (BMOL1.2, BMOL-TAT) were stained using immunofluorescence to determine expression of LPC marker Osteopontin (OPN) (red). IgG isotype control images obtained using identical imaging conditions. DAPI, blue. (B) LPCs (BMOL1.2) were shown to express full-length primary cilium (Pc) structures by immunofluorescence detection of axoneme ( alpha -acetylated tubulin, green) and basal body ( gamma -tubulin, red) markers. This was also confirmed by scanning electron microscopy (SEM). BMOL-TAT cells were also confirmed to express Pc via immunofluorescence and EM studies (data not shown). (C) Nuclear GLI2 (red) expression in LPC lines (BMOL1.2, BMOL-TAT) via immunofluorescence staining. DAPI, blue. Confocal microscopy, 63x objective. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0171480), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Osteopontin/OPN by Western Blot View Larger

Detection of Mouse Osteopontin/OPN by Western Blot Osteopontin (OPN) drives enhancement in macrophage (Mφ) M2 polarization and angiogenic capacity. (A) Representative images of protein expression profiles obtained by comprehensive protein array in each Mφ subset. Red arrowheads indicate OPN. (B) The mRNA expression level of Spp1 relative to glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was analyzed by real-time reverse transcription polymerase chain reaction in each Mφ subset and was normalized to Mφ (–), n = 6 [***p < 0.001 vs. untreated, #p < 0.05 vs. interleukin (IL)-10 alone]. (C) The protein expression level of OPN relative to GAPDH was measured by western blotting and was normalized to Mφ (–), n = 10. Lower panels are typical images of each protein (***p < 0.001 vs. untreated, #p < 0.05 vs. IL-10 alone). (D) Representative confocal laser scanning immunofluorescence overlay images of OPN (red) and DAPI (blue) in each Mφ subset. Scale bar represents 20 µm. Images in the right row are magnified regions from white or yellow rectangles in the panels of corresponding groups. Scale bar represents 10 µm. (E) Relative mean fluorescence intensity (MFI) of CD163 was measured by FACS analysis in each Mφ subset. An anti-OPN antibody (Ab) and its isotype-matched control Ab were used at 3 µg/mL, n = 4 (***p < 0.001 vs. untreated, ##p < 0.01, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). (F) The total areas and lengths of tube-like structures were determined by the Matrigel tube formation assay where b.End5 was cocultured with each Mφ subset, n = 12 (***p < 0.001, **p < 0.01, *p < 0.05 vs. untreated, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). All data are expressed as means ± SEM and were analyzed by a one-way ANOVA followed by Tukey’s test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29559970), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence Thrombin contributes to macrophage (Mφ) M2 polarization and angiogenic capacity through proteolytic modification for osteopontin (OPN). (A) The mRNA expression level of Prothrombin relative to glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was analyzed by reverse transcription polymerase chain reaction in each Mφ subset and were normalized to Mφ (–), n = 7 [***p < 0.001, **p < 0.01 vs. untreated, ##p < 0.01 vs. interleukin (IL)-10 alone]. (B,C) The protein expression levels of (B) thrombin or (C) OPN N-Half relative to GAPDH were measured by western blotting in each Mφ subset and were normalized to Mφ (–). Lower panels are typical images of each protein. (B)n = 8 (***p < 0.001, *p < 0.05 vs. untreated, #p < 0.05 vs. IL-10 alone). (C)n = 16 (***p < 0.001, *p < 0.05 vs. untreated, ##p < 0.01, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). (D) Representative confocal laser scanning immunofluorescence images of OPN (red), thrombin (green), and their merge with DAPI (blue) in each Mφ subset. Scale bar represents 20 µm. Higher magnification images are from the white rectangle region in merged panel of Mφ (IL-10 + IL-18). Scale bar represents 10 µm. (E) Relative mean fluorescence intensity (MFI) of CD163 was measured by FACS analysis in each Mφ subset. Hirudin, a specific thrombin inhibitor, was used at 1 µg/mL, n = 3 (***p < 0.001 vs. untreated, ###p < 0.001 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). (F) The total areas and lengths of tube-like structures were determined by the Matrigel tube formation assay where b.End5 were cocultured with each Mφ subset. Hirudin was used at 1 µg/mL, n = 6 (***p < 0.001, **p < 0.01 vs. untreated, ##p < 0.01, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). All data are expressed as means ± SEM and were analyzed by a one-way ANOVA followed by Tukey’s test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29559970), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence Osteopontin (OPN) drives enhancement in macrophage (Mφ) M2 polarization and angiogenic capacity. (A) Representative images of protein expression profiles obtained by comprehensive protein array in each Mφ subset. Red arrowheads indicate OPN. (B) The mRNA expression level of Spp1 relative to glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was analyzed by real-time reverse transcription polymerase chain reaction in each Mφ subset and was normalized to Mφ (–), n = 6 [***p < 0.001 vs. untreated, #p < 0.05 vs. interleukin (IL)-10 alone]. (C) The protein expression level of OPN relative to GAPDH was measured by western blotting and was normalized to Mφ (–), n = 10. Lower panels are typical images of each protein (***p < 0.001 vs. untreated, #p < 0.05 vs. IL-10 alone). (D) Representative confocal laser scanning immunofluorescence overlay images of OPN (red) and DAPI (blue) in each Mφ subset. Scale bar represents 20 µm. Images in the right row are magnified regions from white or yellow rectangles in the panels of corresponding groups. Scale bar represents 10 µm. (E) Relative mean fluorescence intensity (MFI) of CD163 was measured by FACS analysis in each Mφ subset. An anti-OPN antibody (Ab) and its isotype-matched control Ab were used at 3 µg/mL, n = 4 (***p < 0.001 vs. untreated, ##p < 0.01, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). (F) The total areas and lengths of tube-like structures were determined by the Matrigel tube formation assay where b.End5 was cocultured with each Mφ subset, n = 12 (***p < 0.001, **p < 0.01, *p < 0.05 vs. untreated, #p < 0.05 vs. IL-10 alone, †††p < 0.001 vs. IL-10 + IL-18). All data are expressed as means ± SEM and were analyzed by a one-way ANOVA followed by Tukey’s test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29559970), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Osteopontin/OPN by Immunocytochemistry/Immunofluorescence Localized differentiation of liver progenitors in arrayed patterns.(A) Immunolabeling of BMEL cells for the biliary marker OPN and hepatocyte marker ALB on arrayed collagen I patterns with control IgG or Fc-recombinant Notch ligands DLL1, DLL4, and JAG1. (B) Quantification of OPN+ cell counts as a function of radial distance from the centroid of each island. (C) Quantification of ALB+ cell counts as a function of radial distance from the centroid of each island. (D) Immunolabeling of BMEL cells presented with DLL4 for the biliary transcription factor SOX9 and hepatocyte transcription factor HNF4A. Arrow in each image indicates the same SOX9+/HNF4A− cell. Scale bar indicates 75 µm. (E, F) Regression analysis of OPN+ and ALB+ cell counts. Data in Figure 1B and Figure 1C were separated into peripheral and central subsets for which dimensionless radius was greater than 0.75 (R>0.75) and less than 0.75 (R<0.75). Separate multiple regression models were generated for each data subset for which coefficient estimates (corresponding to mean change in cell counts) and 95% CI were plotted for OPN+ (E) and ALB+ (F) cells. For each factor, 95% CI that do not intersect with the dashed line indicate regression coefficient estimates for which P<0.05. (A, E) Scale bars indicate 150 µm.10.7554/eLife.38536.007Figure 1—source data 1.Summary table for OPN data in Figure 1B.10.7554/eLife.38536.008Figure 1—source data 2.Summary table for ALB data in Figure 1C.Summary table for OPN data in Figure 1B.Summary table for ALB data in Figure 1C.Immunolabeling and quantification of CK19.(A) Immunolabeling of BMEL cells presented with IgG for the biliary marker CK19. (B) Quantification of CK19 intensity in BMEL cells presented with IgG as a function of radial distance from the centroid of each island. Asterisk (*) indicates P<0.001 for peak intensity (R>0.9) compared with central intensity (R<0.1) using Welch’s t-test.Immunolabeling of OPN and CK19 at t=24h and cell density with radius at t=72h.(A) Immunolabeling at t=24h of BMEL cells on 30 kPa substrates presented with IgG and DLL4 for the biliary markers OPN and CK19. Scale bar indicates 75 µm. (B) Measurement of cell density with radius for 30 kPa and 4 kPa substrates at t=72h. Vertical black bars indicate computed mean radius using the most central 95% of cells within the data set.Immunolabeling for OPN with 300, 600, and 1000 µm diameter patterns.(A) Immunolabeling for OPN of BMEL cells presented with DLL4 on 30 kPa substrates at t=72h. Both 300 µm and 1000 µm pattern diameters were included in this experiment in addition to the 600 µm pattern diameter. Scale bars indicate 150 µm. (B) Quantification of peak OPN+ cell counts for 300, 600, and 1000 µm diameter pattern. Boxplots show median, 25th and 75th percentiles (hinges), and 1.5 × IQR (whiskers). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30589410), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteopontin/OPN

Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Mouse OPN cDNA encodes a 294 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 278 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha v beta 1, alpha v beta 3, alpha v beta 5, and non-RGD-mediated interaction with CD44 and the integrins alpha 8 beta 1 or alpha 9 beta 1. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40-80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

References
  1. Ann. N.Y. Acad. Sci., vol. 760, 1995, Apr. 21.
  2. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
  3. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.
Long Name
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin

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Citations for Mouse Osteopontin/OPN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

207 Citations: Showing 1 - 10
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  1. Impaired disassembly of the axon initial segment restricts mitochondrial entry into damaged axons
    Authors: Kiryu-Seo S, Matsushita R, Tashiro Y et al.
    The EMBO journal
  2. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping
    Authors: N Severe, NM Karabacak, K Gustafsson, N Baryawno, G Courties, Y Kfoury, KD Kokkaliari, C Rhee, D Lee, EW Scadden, JE Garcia-Rob, T Brouse, M Nahrendorf, M Toner, DT Scadden
    Cell Stem Cell, 2019-07-03;0(0):.
  3. Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration
    Authors: Guo Y, Chomiak AA, Hong Y et al.
    iScience
  4. Contributions of mirror-image hair cell orientation to mouse otolith organ and zebrafish neuromast function
    Authors: Ono, K;Jarysta, A;Hughes, NC;Jukic, A;Chang, HHV;Deans, MR;Eatock, RA;Cullen, KE;Kindt, K;Tarchini, B;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  5. Osteopontin deletion attenuates cyst growth but exacerbates fibrosis in mice with cystic kidney disease
    Authors: Jansson, KP;Kuluva, J;Zhang, S;Swanson, T;Zhang, Y;Zimmerman, KA;Fields, TA;Wallace, DP;Rowe, PS;Stubbs, JR;
    Physiological reports
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  6. Modulating amacrine cell-derived dopamine signaling promotes optic nerve regeneration and preserves visual function
    Authors: Zhang, Q;Xue, J;Tang, J;Wu, S;Liu, Z;Wu, C;Liu, C;Liu, Y;Lin, J;Han, J;Liu, L;Chen, Y;Yang, J;Li, Z;Zhao, L;Wei, Y;Li, Y;Zhuo, Y;
    Science advances
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Immunohistochemistry
  7. Spike desensitisation as a mechanism for high-contrast selectivity in retinal ganglion cells
    Authors: Chang, L;Ran, Y;Yang, M;Auferkorte, O;Butz, E;Hüser, L;Haverkamp, S;Euler, T;Schubert, T;
    Frontiers in cellular neuroscience
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Mitochondrial reverse electron transport in myeloid cells perpetuates neuroinflammation
    Authors: Peruzzotti-Jametti, L;Willis, CM;Hamel, R;Krzak, G;Reisz, JA;Prag, HA;Wu, V;Xiang, Y;van den Bosch, AMR;Nicaise, AM;Roth, L;Bates, GR;Huang, H;Vincent, AE;Frezza, C;Viscomi, C;Marioni, JC;D'Alessandro, A;Takats, Z;Murphy, MP;Pluchino, S;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  9. A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons
    Authors: Winter, CC;Jacobi, A;Su, J;Chung, L;van Velthoven, CTJ;Yao, Z;Lee, C;Zhang, Z;Yu, S;Gao, K;Duque Salazar, G;Kegeles, E;Zhang, Y;Tomihiro, MC;Zhang, Y;Yang, Z;Zhu, J;Tang, J;Song, X;Donahue, RJ;Wang, Q;McMillen, D;Kunst, M;Wang, N;Smith, KA;Romero, GE;Frank, MM;Krol, A;Kawaguchi, R;Geschwind, DH;Feng, G;Goodrich, LV;Liu, Y;Tasic, B;Zeng, H;He, Z;
    Nature
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Multisensory gaze stabilization in response to subchronic alteration of vestibular type I hair cells
    Authors: Schenberg, L;Palou, A;Simon, F;Bonnard, T;Barton, CE;Fricker, D;Tagliabue, M;Llorens, J;Beraneck, M;
    eLife
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Bioactive glasses promote rapid pre-osteoblastic cell migration in contrast to hydroxyapatite, while carbonated apatite shows migration inhibiting properties
    Authors: Kajander, K;Sirkiä, SV;Vallittu, PK;Heino, TJ;Määttä, JA;
    Scientific reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Lymphatic platelet thrombosis limits bone repair by precluding lymphatic transporting DAMPs
    Authors: Wang, YJ;Zheng, Y;Cong, L;Wang, P;Zhao, L;Xing, L;Liu, J;Xu, H;Li, N;Zhao, Y;Shi, Q;Liang, Q;
    Research square
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Decoding muscle-resident Schwann cell dynamics during neuromuscular junction remodeling
    Authors: Guzman, SD;Abu-Mahfouz, A;Davis, CS;Ruiz, LP;Macpherson, PC;Brooks, SV;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. TM4SF5-mediated abnormal food-intake behavior and apelin expression facilitate non-alcoholic fatty liver disease features
    Authors: Yangie Dwi Pinanga, Han Ah Lee, Eun-Ae Shin, Haesong Lee, Kyung-hee Pyo, Ji Eon Kim et al.
    iScience
  15. Coordination between ECM and cell-cell adhesion regulates the development of islet aggregation, architecture, and functional maturation
    Authors: Tixi W, Maldonado M, Chang YT et al.
    eLife
  16. Osteopontin drives retinal ganglion cell resiliency in glaucomatous optic neuropathy
    Authors: Zhao, M;Toma, K;Kinde, B;Li, L;Patel, AK;Wu, KY;Lum, MR;Tan, C;Hooper, JE;Kriegstein, AR;La Torre, A;Liao, YJ;Welsbie, DS;Hu, Y;Han, Y;Duan, X;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Different niches for stem cells carrying the same oncogenic driver affect pathogenesis and therapy response in myeloproliferative neoplasms
    Authors: Elodie Grockowiak, Claudia Korn, Justyna Rak, Veronika Lysenko, Adrien Hallou, Francesca M. Panvini et al.
    Nature Cancer
  18. Signalling by senescent melanocytes hyperactivates hair growth
    Authors: Wang, X;Ramos, R;Phan, AQ;Yamaga, K;Flesher, JL;Jiang, S;Oh, JW;Jin, S;Jahid, S;Kuan, CH;Nguyen, TK;Liang, HY;Shettigar, NU;Hou, R;Tran, KH;Nguyen, A;Vu, KN;Phung, JL;Ingal, JP;Levitt, KM;Cao, X;Liu, Y;Deng, Z;Taguchi, N;Scarfone, VM;Wang, G;Paolilli, KN;Wang, X;Guerrero-Juarez, CF;Davis, RT;Greenberg, EN;Ruiz-Vega, R;Vasudeva, P;Murad, R;Widyastuti, LHP;Lee, HL;McElwee, KJ;Gadeau, AP;Lawson, DA;Andersen, B;Mortazavi, A;Yu, Z;Nie, Q;Kunisada, T;Karin, M;Tuckermann, J;Esko, JD;Ganesan, AK;Li, J;Plikus, MV;
    Nature
    Species: Transgenic Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: Immunohistochemistry, Western Blot
  19. Interleukin-15 deficient rats have reduced osteopontin at the maternal-fetal interface
    Authors: Kelly J. Baines, Michelle S. Klausner, Violet S. Patterson, Stephen J. Renaud
    Frontiers in Cell and Developmental Biology
  20. Peroxisomal defects in microglial cells induce a disease-associated microglial signature
    Authors: Quentin Raas, Ali Tawbeh, Mounia Tahri-Joutey, Catherine Gondcaille, Céline Keime, Romain Kaiser et al.
    Frontiers in Molecular Neuroscience
  21. NOX4 as a critical effector mediating neuroinflammatory cytokines, myeloperoxidase and osteopontin, specifically in astrocytes in the hippocampus in Parkinson's disease
    Authors: N Boonpraman, S Yoon, CY Kim, JS Moon, SS Yi
    Redox Biology, 2023-04-10;62(0):102698.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  22. Hepatic lipid overload triggers biliary epithelial cell activation via E2Fs
    Authors: E Yildiz, G El Alam, A Perino, A Jalil, PD Denechaud, K Huber, L Fajas, J Auwerx, G Sorrentino, K Schoonjans
    Elife, 2023-03-06;12(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Kupffer-cell-derived IL-6 is repurposed for hepatocyte dedifferentiation via activating progenitor genes from injury-specific enhancers
    Authors: L Li, L Cui, P Lin, Z Liu, S Bao, X Ma, H Nan, W Zhu, J Cen, Y Mao, X Ma, L Jiang, Y Nie, F Ginhoux, Y Li, H Li, L Hui
    Cell Stem Cell, 2023-02-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Perivascular cells induce microglial phagocytic states and synaptic engulfment via SPP1 in mouse models of Alzheimer's disease
    Authors: S De Scheppe, JZ Ge, G Crowley, LSS Ferreira, D Garceau, CE Toomey, D Sokolova, J Rueda-Carr, SH Shin, JS Kim, T Childs, T Lashley, JJ Burden, M Sasner, C Sala Frige, S Jung, S Hong
    Nature Neuroscience, 2023-02-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease
    Authors: Y Qiu, X Shen, O Ravid, D Atrakchi, D Rand, AE Wight, HJ Kim, S Liraz-Zalt, I Cooper, M Schnaider, H Cantor
    Proceedings of the National Academy of Sciences of the United States of America, 2023-02-02;120(6):e2218915120.
    Species: Mouse
    Sample Types: Recombinant Protein, Whole Tissue
    Applications: Bioassay, IHC
  26. Vitamin D Receptor Expression Limits the Angiogenic and Inflammatory Properties of Retinal Endothelial Cells
    Authors: YS Song, N Jamali, CM Sorenson, N Sheibani
    Cells, 2023-01-16;12(2):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling
    Authors: Q Bai, X Wang, H Yan, L Wen, Z Zhou, Y Ye, Y Jing, Y Niu, L Wang, Z Zhang, J Su, T Chang, G Dou, Y Wang, J Sun
    Journal of personalized medicine, 2023-01-11;13(1):.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  28. Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor indicates a cell-specific response that promotes CD44 activation
    Authors: D Piekna-Prz, D Na, J Zhang, C Baker, JM Ashton, PM White
    Frontiers in Cellular Neuroscience, 2023-01-06;16(0):1096872.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  29. Secreted phosphoprotein 1 slows neurodegeneration and rescues visual function in mouse models of aging and glaucoma
    Authors: S Li, TC Jakobs
    Cell Reports, 2022-12-27;41(13):111880.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  30. Human Recombinant Lactoferrin Promotes Differentiation and Calcification on MC3T3-E1 Cells
    Authors: D Nagashima, Y Ishibashi, S Kawaguchi, M Furukawa, M Toho, M Ohno, T Nitto, N Izumo
    Pharmaceutics, 2022-12-25;15(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  31. Discoidin domain receptor 2 regulates aberrant mesenchymal lineage cell fate and matrix organization
    Authors: CA Pagani, AC Bancroft, RJ Tower, N Livingston, Y Sun, JY Hong, RN Kent, AL Strong, JH Nunez, JMR Medrano, N Patel, BA Nanes, KM Dean, Z Li, C Ge, BM Baker, AW James, SJ Weiss, RT Franceschi, B Levi
    Science Advances, 2022-12-21;8(51):eabq6152.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  32. Anti-osteopontin therapy leads to improved edema and infarct size in a murine model of ischemic stroke
    Authors: D Spitzer, T Puetz, M Armbrust, M Dunst, J Macas, F Croll, KH Plate, Y Reiss, S Liebner, PN Harter, S Guérit, K Devraj
    Scientific Reports, 2022-12-03;12(1):20925.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Cell Culture, In Vivo
  33. cis interaction of CD153 with TCR/CD3 is crucial for the pathogenic activation of senescence-associated T�cells
    Authors: Y Fukushima, K Sakamoto, M Matsuda, Y Yoshikai, H Yagita, D Kitamura, M Chihara, N Minato, M Hattori
    Cell Reports, 2022-09-20;40(12):111373.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro
    Authors: T Patel, J Hammelman, S Aziz, S Jang, M Closser, TL Michaels, JA Blum, DK Gifford, H Wichterle
    Nature Communications, 2022-09-15;13(1):5427.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  35. Regulating microglial miR-155 transcriptional phenotype alleviates Alzheimer's-induced retinal vasculopathy by limiting Clec7a/Galectin-3+ neurodegenerative microglia
    Authors: H Shi, Z Yin, Y Koronyo, DT Fuchs, J Sheyn, MR Davis, JW Wilson, MA Margeta, KM Pitts, S Herron, S Ikezu, T Ikezu, SL Graham, VK Gupta, KL Black, M Mirzaei, O Butovsky, M Koronyo-Ha
    Acta neuropathologica communications, 2022-09-08;10(1):136.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. Cilostazol Attenuates AngII-Induced Cardiac Fibrosis in apoE Deficient Mice
    Authors: Y Hada, HA Uchida, R Umebayashi, M Yoshida, J Wada
    International Journal of Molecular Sciences, 2022-08-13;23(16):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. TET enzymes regulate skeletal development through increasing chromatin accessibility of RUNX2 target genes
    Authors: L Wang, X You, D Ruan, R Shao, HQ Dai, W Shen, GL Xu, W Liu, W Zou
    Nature Communications, 2022-08-11;13(1):4709.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  38. Profiling the neurovascular unit unveils detrimental effects of osteopontin on the blood–brain barrier in acute ischemic stroke
    Authors: Daniel Spitzer, Sylvaine Guérit, Tim Puetz, Maryam I. Khel, Moritz Armbrust, Maika Dunst et al.
    Acta Neuropathologica
  39. INZ‐701, a recombinant ENPP1 enzyme, prevents ectopic calcification in an Abcc6−/− mouse model of pseudoxanthoma elasticum
    Authors: Ida Joely Jacobs, Zhiliang Cheng, Douglas Ralph, Kevin O'Brien, Lisa Flaman, Jennifer Howe et al.
    Experimental Dermatology
  40. Intermittent parathyroid hormone increases stability and improves osseointegration of initially unstable implants
    Authors: Kevin Staats, Branden R. Sosa, Emile-Victor Kuyl, Yingzhen Niu, Vincentius Suhardi, Kathleen Turajane et al.
    Bone & Joint Research
  41. Loss of mouse Stmn2 function causes motor neuropathy
    Authors: Irune Guerra Guerra San Juan, Leslie A. Nash, Kevin S. Smith, Marcel F. Leyton-Jaimes, Menglu Qian, Joseph R. Klim et al.
    Neuron
  42. Vertebrate lonesome kinase modulates the hepatocyte secretome to prevent perivascular liver fibrosis and inflammation
    Authors: S Pantasis, J Friemel, SM Brütsch, Z Hu, S Krautbauer, G Liebisch, J Dengjel, A Weber, S Werner, MR Bordoli
    Journal of Cell Science, 2022-04-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  43. Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration
    Authors: S Lin, S Yin, J Shi, G Yang, X Wen, W Zhang, M Zhou, X Jiang
    Bioactive materials, 2022-03-24;18(0):116-127.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  44. Transcriptional and Distributional Profiling of Microglia in Retinal Angiomatous Proliferation
    Authors: A Schlecht, J Wolf, S Boneva, G Prinz, BM Braunger, P Wieghofer, H Agostini, G Schlunck, C Lange
    International Journal of Molecular Sciences, 2022-03-22;23(7):.
    Species: Mouse
    Sample Types: In Vivo
  45. Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure
    Authors: FZ Khamissi, L Ning, E Kefaloyian, H Dun, A Arthanaris, A Keller, JJ Atkinson, W Li, B Wong, S Dietmann, K Lavine, D Kreisel, A Herrlich
    Science Advances, 2022-02-25;8(8):eabm5900.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  46. Hypertrophic chondrocytes serve as a reservoir for marrow-associated skeletal stem and progenitor cells, osteoblasts, and adipocytes during skeletal development
    Authors: Jason T Long, Abigail Leinroth, Yihan Liao, Yinshi Ren, Anthony J Mirando, Tuyet Nguyen et al.
    eLife
  47. Induction of osteogenesis by bone-targeted Notch activation
    Authors: C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams
    Elife, 2022-02-04;11(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. Increased expression of osteopontin in subchondral bone promotes bone turnover and remodeling, and accelerates the progression of OA in a mouse model
    Authors: Chuangxin Lin, Zhong Chen, Dong Guo, Laixi Zhou, Sipeng Lin, Changchuan Li et al.
    Aging (Albany NY)
  49. Secreted osteopontin from CD4+ T�cells limits acute graft-versus-host disease
    Authors: N Aggarwal, ME Deerhake, D DiPalma, SK Shahi, MR Gaggioli, AK Mangalam, ML Shinohara
    Cell Reports, 2021-12-28;37(13):110170.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  50. STAT3 is critical for skeletal development and bone homeostasis by regulating osteogenesis
    Authors: S Zhou, Q Dai, X Huang, A Jin, Y Yang, X Gong, H Xu, X Gao, L Jiang
    Nature Communications, 2021-11-25;12(1):6891.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  51. Osteopontin Deficiency Ameliorates Prostatic Fibrosis and Inflammation
    Authors: P Popovics, A Jain, KO Skalitzky, E Schroeder, H Ruetten, M Cadena, KS Uchtmann, CM Vezina, WA Ricke
    International Journal of Molecular Sciences, 2021-11-18;22(22):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  52. Development and Characterization of Alkaline Phosphatase-Positive Human Umbilical Cord Perivascular Cells
    Authors: S Nonoyama, T Karakida, R Chiba-Ohku, R Yamamoto, Y Ujiie, T Nagano, Y Yamakoshi, K Gomi
    Cells, 2021-11-04;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  53. Serotonin transporter-mediated molecular axis regulates regional retinal ganglion cell vulnerability and axon regeneration after nerve injury
    Authors: R Kingston, D Amin, S Misra, JM Gross, T Kuwajima
    PloS Genetics, 2021-11-04;17(11):e1009885.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  54. 7,8-Dihydroxiflavone Maintains Retinal Functionality and Protects Various Types of RGCs in Adult Rats with Optic Nerve Transection
    Authors: Alejandro Gallego-Ortega, Beatriz Vidal-Villegas, María Norte-Muñoz, Manuel Salinas-Navarro, Marcelino Avilés-Trigueros, María Paz Villegas-Pérez et al.
    International Journal of Molecular Sciences
  55. Creation of X-linked Alport syndrome rat model with Col4a5 deficiency
    Authors: M Namba, T Kobayashi, M Kohno, T Koyano, T Hirose, M Fukushima, M Matsuyama
    Scientific Reports, 2021-10-21;11(1):20836.
    Species: Rat
    Sample Types: Tissue Lysates
    Applications: Western Blot
  56. Quantitative Expression of Key Cancer Markers in the AS-30D Hepatocarcinoma Model
    Authors: Marco A. Briones-Orta, Blanca Delgado-Coello, Roxana Gutiérrez-Vidal, Marcela Sosa-Garrocho, Marina Macías-Silva, Jaime Mas-Oliva
    Frontiers in Oncology
  57. IER2-induced senescence drives melanoma invasion through osteopontin
    Authors: L Kyjacova, R Saup, K Rönsch, S Wallbaum, S Dukowic-Sc, A Foss, SD Scherer, M Rothley, A Neeb, N Grau, W Thiele, S Thaler, N Cremers, C Sticht, N Gretz, BK Garvalov, J Utikal, JP Sleeman
    Oncogene, 2021-10-05;0(0):.
    Species: Mouse
    Sample Types: CellLysates, Whole Tissue
    Applications: IHC, Western Blot
  58. Differential Response of M�ller Cells and Microglia in a Mouse Retinal Detachment Model and Its Implications in Detached and Non-Detached Regions
    Authors: SH Lee, YS Park, SS Paik, IB Kim
    Cells, 2021-08-03;10(8):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  59. Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation
    Authors: L Cordero-Es, AM Dowbaj, TN Kohler, B Strauss, O Sarlidou, G Belenguer, C Pacini, NP Martins, R Dobie, JR Wilson-Kan, R Butler, N Prior, P Serup, F Jug, NC Henderson, F Hollfelder, M Huch
    Cell Stem Cell, 2021-08-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  60. SLITRK5 is a negative regulator of hedgehog signaling in osteoblasts
    Authors: J Sun, DY Shin, M Eiseman, AR Yallowitz, N Li, S Lalani, Z Li, M Cung, S Bok, S Debnath, SJ Marquez, TE White, AG Khan, IC Lorenz, JH Shim, FS Lee, R Xu, MB Greenblatt
    Nature Communications, 2021-07-29;12(1):4611.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  61. WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escape
    Authors: Chunwan Lu, Zhuoqi Liu, John D Klement, Dafeng Yang, Alyssa D Merting, Dakota Poschel et al.
    Journal for ImmunoTherapy of Cancer
  62. Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics
    Authors: TA Jan, Y Eltawil, AH Ling, L Chen, DC Ellwanger, S Heller, AG Cheng
    Cell Reports, 2021-07-13;36(2):109358.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC, RNAScope
  63. Regional specialization and fate specification of bone stromal cells in skeletal development
    Authors: KK Sivaraj, HW Jeong, B Dharmaling, D Zeuschner, S Adams, M Potente, RH Adams
    Cell Reports, 2021-07-13;36(2):109352.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  64. Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity
    Authors: Akitoshi Hara, Katsuhiro Kato, Toshikazu Ishihara, Hiroki Kobayashi, Naoya Asai, Shinji Mii et al.
    Genes to Cells
  65. Microtissue Geometry and Cell‐Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D
    Authors: Ian C. Berg, Erfan Mohagheghian, Krista Habing, Ning Wang, Gregory H. Underhill
    Advanced Healthcare Materials
  66. EMX2-GPR156-G&alphai reverses hair cell orientation in mechanosensory epithelia
    Authors: KS Kindt, A Akturk, A Jarysta, M Day, A Beirl, M Flonard, B Tarchini
    Nature Communications, 2021-05-17;12(1):2861.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  67. MyD88 in myofibroblasts enhances colitis-associated tumorigenesis via promoting macrophage M2 polarization
    Authors: Q Yuan, J Gu, J Zhang, S Liu, Q Wang, T Tian, Z Chen, J Zhang
    Cell Reports, 2021-02-02;34(5):108724.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  68. Secreted Phosphoprotein 1 Expression in Retinal Mononuclear Phagocytes Links Murine to Human Choroidal Neovascularization
    Authors: Anja Schlecht, Peipei Zhang, Julian Wolf, Adrian Thien, Dennis-Dominik Rosmus, Stefaniya Boneva et al.
    Frontiers in Cell and Developmental Biology
  69. The role of extracellular matrix phosphorylation on energy dissipation in bone
    Authors: Stacyann Bailey, Grazyna E Sroga, Betty Hoac, Orestis L Katsamenis, Zehai Wang, Nikolaos Bouropoulos et al.
    eLife
  70. Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility
    Authors: L Yu, Y Zheng, BJ Liu, MH Kang, JC Millar, DJ Rhee
    PLoS ONE, 2020-11-04;15(11):e0241294.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  71. Tendon-derived cathepsin K–expressing progenitor cells activate Hedgehog signaling to drive heterotopic ossification
    Authors: Heng Feng, Wenhui Xing, Yujiao Han, Jun Sun, Mingxiang Kong, Bo Gao et al.
    Journal of Clinical Investigation
  72. Microglia-organized scar-free spinal cord repair in neonatal mice
    Authors: Y Li, X He, R Kawaguchi, Y Zhang, Q Wang, A Monavarfes, Z Yang, B Chen, Z Shi, H Meng, S Zhou, J Zhu, A Jacobi, V Swarup, PG Popovich, DH Geschwind, Z He
    Nature, 2020-10-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  73. Ectopic bone formation and systemic bone loss in a transmembrane TNF-driven model of human spondyloarthritis
    Authors: E Christodou, C Geka, L Ntari, K Kranidioti, E Argyropoul, F Meier, M Armaka, I Mourouzis, C Pantos, M Rouchota, G Loudos, MC Denis, N Karagianni, G Kollias
    Arthritis Res Ther, 2020-10-06;22(1):232.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  74. Painting the Pancreas in Three Dimensions: Whole-Mount Immunofluorescence Method
    Authors: Maricela Maldonado, Jeffrey D. Serrill, Hung-Ping Shih
    Stem Cells and Tissue Repair
  75. Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection
    Authors: FJ Mahmud, Y Du, E Greif, T Boucher, RF Dannals, WB Mathews, MG Pomper, P Sysa-Shah, KA Metcalf Pa, C Lyons, B Carlson, M Chacona, AM Brown
    Journal of Neuroinflammation, 2020-09-17;17(1):273.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  76. Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver
    Authors: Anneleen Remmerie, Liesbet Martens, Tinne Thoné, Angela Castoldi, Ruth Seurinck, Benjamin Pavie et al.
    Immunity
  77. Loss of Osteopontin Expression Reduces HSV-1-Induced Corneal Opacity
    Authors: A Filiberti, GB Gmyrek, ML Montgomery, R Sallack, DJJ Carr
    Invest. Ophthalmol. Vis. Sci., 2020-08-03;61(10):24.
    Species: Mouse, Transgenic Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  78. Integration of Hydrogel Microparticles With Three-Dimensional Liver Progenitor Cell Spheroids
    Authors: Stefan D. Gentile, Andreas P. Kourouklis, Hyeon Ryoo, Gregory H. Underhill
    Frontiers in Bioengineering and Biotechnology
  79. Modular output circuits of the fastigial nucleus for diverse motor and nonmotor functions of the cerebellar vermis
    Authors: Hirofumi Fujita, Takashi Kodama, Sascha du Lac
    eLife
  80. Impact of osteopontin on the development of non‐alcoholic liver disease and related hepatocellular carcinoma
    Authors: Alexander D. Nardo, Nicole G. Grün, Maximilian Zeyda, Monika Dumanic, Georg Oberhuber, Elisa Rivelles et al.
    Liver International
  81. Snai2 Maintains Bone Marrow Niche Cells by Repressing Osteopontin Expression
    Authors: Qiaozhi Wei, Fumio Nakahara, Noboru Asada, Dachuan Zhang, Xin Gao, Chunliang Xu et al.
    Developmental Cell
  82. Fgf8 genetic labeling reveals the early specification of vestibular hair cell type in mouse utricle
    Authors: Evan M. Ratzan, Anne M. Moon, Michael R. Deans
    Development
  83. Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells
    Authors: J Falero-Per, N Sheibani, CM Sorenson
    PLoS ONE, 2020-05-04;15(5):e0232779.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  84. Adeno-Associated Virus Serotype 8-Mediated Genetic Labeling of Cholangiocytes in the Neonatal Murine Liver
    Authors: Sanghoon Lee, Ping Zhou, Senyo Whyte, Soona Shin
    Pharmaceutics
  85. Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-?v&beta6 integrin in liver fibrosis
    Authors: T Xu, Z Lu, Z Xiao, F Liu, Y Chen, Z Wang, S Zhu, Y Song
    Cell Death Dis, 2020-03-23;11(3):199.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  86. Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-beta -catenin
    Authors: Taifeng Zhou, Bo Gao, Yi Fan, Yuchen Liu, Shuhao Feng, Qian Cong et al.
    eLife
  87. Hox genes maintain critical roles in the adult skeleton
    Authors: JY Song, KM Pineault, JM Dones, RT Raines, DM Wellik
    Proc. Natl. Acad. Sci. U.S.A., 2020-03-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  88. Vascular endothelial growth factor pathway promotes osseointegration and CD31hiEMCNhi endothelium expansion in a mouse tibial implant model: an animal study
    Authors: G. Ji, R. Xu, Y. Niu, N. Li, L. Ivashkiv, M. P. G. Bostrom et al.
    The Bone & Joint Journal
  89. Activating Transcription Factor 3 (ATF3) Protects Retinal Ganglion Cells and Promotes Functional Preservation After Optic Nerve Crush
    Authors: C Kole, B Brommer, N Nakaya, M Sengupta, L Bonet-Ponc, T Zhao, C Wang, W Li, Z He, S Tomarev
    Invest. Ophthalmol. Vis. Sci., 2020-02-07;61(2):31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  90. Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells
    Authors: DJ Fu, L Wang, FK Chouairi, IM Rose, DA Abetov, AD Miller, RJ Yamulla, JC Schimenti, A Flesken-Ni, AY Nikitin
    Nat Commun, 2020-01-03;11(1):84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  91. Retinoic acid degradation shapes zonal development of vestibular organs and sensitivity to transient linear accelerations
    Authors: Kazuya Ono, James Keller, Omar López Ramírez, Antonia González Garrido, Omid A. Zobeiri, Hui Ho Vanessa Chang et al.
    Nature Communications
  92. Osteopontin and its spatiotemporal relationship with glial cells in the striatum of rats treated with mitochondrial toxin 3-nitropropionic acid: possible involvement in phagocytosis
    Authors: Tae-Ryong Riew, Soojin Kim, Xuyan Jin, Hong Lim Kim, Jeong-Hwa Lee, Mun-Yong Lee
    Journal of Neuroinflammation
  93. Single-Cell Profiles of Retinal Ganglion Cells Differing in Resilience to Injury Reveal Neuroprotective Genes
    Authors: Nicholas M. Tran, Karthik Shekhar, Irene E. Whitney, Anne Jacobi, Inbal Benhar, Guosong Hong et al.
    Neuron
  94. Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury
    Authors: D Feng, C Ngov, N Henley, N Boufaied, C Gerarduzzi
    Sci Rep, 2019-11-13;9(1):16736.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  95. Inactivation of mTORC2 in macrophages is a signature of colorectal cancer that promotes tumorigenesis
    Authors: Karl Katholnig, Birgit Schütz, Stephanie D. Fritsch, David Schörghofer, Monika Linke, Nyamdelger Sukhbaatar et al.
    JCI Insight
  96. In Vivo Activation and Pro-Fibrotic Function of NF-kappaB in Fibroblastic Cells During Pulmonary Inflammation and Fibrosis Induced by Carbon Nanotubes
    Authors: J Dong, Q Ma
    Front Pharmacol, 2019-10-02;10(0):1140.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  97. ATRQ β-001 Vaccine Prevents Experimental Abdominal Aortic Aneurysms
    Authors: H Zhang, M Liao, M Cao, Z Qiu, X Yan, Y Zhou, H Wu, Y Wang, J Zheng, J Ding, M Wang, Y Liao, X Chen
    J Am Heart Assoc, 2019-09-12;8(18):e012341.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  98. Survival of Alpha and Intrinsically Photosensitive Retinal Ganglion Cells in NMDA-Induced Neurotoxicity and a Mouse Model of Normal Tension Glaucoma
    Authors: S Honda, K Namekata, A Kimura, X Guo, C Harada, A Murakami, A Matsuda, T Harada
    Invest. Ophthalmol. Vis. Sci., 2019-09-03;60(12):3696-3707.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  99. Thrombospondin-1 Mediates Axon Regeneration in Retinal Ganglion Cells
    Authors: Eric R. Bray, Benjamin J. Yungher, Konstantin Levay, Marcio Ribeiro, Gennady Dvoryanchikov, Ana C. Ayupe et al.
    Neuron
  100. Excessive exosome release is the pathogenic pathway linking a lysosomal deficiency to generalized fibrosis
    Authors: D van de Vle, J Demmers, XX Nguyen, Y Campos, E Machado, I Annunziata, H Hu, E Gomero, X Qiu, A Bongiovann, CA Feghali-Bo, A d'Azzo
    Sci Adv, 2019-07-17;5(7):eaav3270.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  101. Atoh1 Directs Regeneration and Functional Recovery of the Mature Mouse Vestibular System
    Authors: ZN Sayyid, T Wang, L Chen, SM Jones, AG Cheng
    Cell Rep, 2019-07-09;28(2):312-324.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  102. Pancreatic Ductal Deletion of Hnf1b Disrupts Exocrine Homeostasis, Leads to Pancreatitis, and Facilitates Tumorigenesis
    Authors: E Quilichini, M Fabre, T Dirami, A Stedman, M De Vas, O Ozguc, RC Pasek, S Cereghini, L Morillon, C Guerra, A Couvelard, M Gannon, C Haumaitre
    Cell Mol Gastroenterol Hepatol, 2019-06-21;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  103. Single-Cell Analysis of the Liver Epithelium Reveals Dynamic Heterogeneity and an Essential Role for YAP in Homeostasis and Regeneration
    Authors: BJ Pepe-Moone, MT Dill, A Alemany, J Ordovas-Mo, Y Matsushita, A Rao, A Sen, M Miyazaki, S Anakk, PA Dawson, N Ono, AK Shalek, A van Oudena, FD Camargo
    Cell Stem Cell, 2019-05-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  104. Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation
    Authors: SG Romeo, KM Alawi, J Rodrigues, A Singh, AP Kusumbe, SK Ramasamy
    Nat. Cell Biol., 2019-04-01;21(4):430-441.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  105. Pulmonary arterial remodelling by deficiency of peroxisome proliferator-activated receptor-? in murine vascular smooth muscle cells occurs independently of obesity-related pulmonary hypertension
    Authors: E Caglayan, M Trappiel, A Behringer, EM Berghausen, M Odenthal, E Wellnhofer, K Kappert
    Respir. Res., 2019-02-27;20(1):42.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  106. Normal inflammation and regeneration of muscle following injury require osteopontin from both muscle and non-muscle cells
    Authors: DK Wasgewatte, EJ Mackie, CN Pagel
    Skelet Muscle, 2019-02-26;9(1):6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  107. Osteopontin in the host response to Leishmania amazonensis
    Authors: E Giraud, E Rouault, L Fiette, JH Colle, D Smirlis, E Melanitou
    BMC Microbiol., 2019-02-08;19(1):32.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot
  108. N-Cadherin-Expressing Bone and Marrow Stromal Progenitor Cells Maintain Reserve Hematopoietic Stem Cells
    Authors: M Zhao, F Tao, A Venkatrama, Z Li, SE Smith, J Unruh, S Chen, C Ward, P Qian, JM Perry, H Marshall, J Wang, XC He, L Li
    Cell Rep, 2019-01-15;26(3):652-669.e6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  109. Correlation of OPN gene expression with proliferation and apoptosis of ovarian cancer cells and prognosis of patients
    Authors: H Hu, Z Liu, C Liu
    Oncol Lett, 2019-01-07;17(3):2788-2794.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  110. Developmental Heterogeneity of Microglia and Brain Myeloid Cells Revealed by Deep Single-Cell RNA Sequencing
    Authors: Qingyun Li, Zuolin Cheng, Lu Zhou, Spyros Darmanis, Norma F. Neff, Jennifer Okamoto et al.
    Neuron
  111. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling
    Authors: KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill
    Elife, 2018-12-27;7(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  112. Characterization of spatial and temporal development of Type I and Type II hair cells in the mouse utricle using new cell-type-specific markers
    Authors: Stephen McInturff, Joseph C. Burns, Matthew W. Kelley
    Biology Open
  113. H3K36 trimethylation mediated by SETD2 regulates the fate of bone marrow mesenchymal stem cells
    Authors: L Wang, N Niu, L Li, R Shao, H Ouyang, W Zou
    PLoS Biol., 2018-11-13;16(11):e2006522.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  114. Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression
    Authors: MN Lee, HS Hwang, SH Oh, A Roshanzade, JW Kim, JH Song, ES Kim, JT Koh
    Exp. Mol. Med., 2018-11-05;50(11):142.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  115. Molecular fingerprinting of On-Off direction selective retinal ganglion cells across species and relevance to primate visual circuits
    Authors: OS Dhande, BK Stafford, K Franke, R El-Danaf, KA Percival, AH Phan, P Li, BJ Hansen, PL Nguyen, P Berens, WR Taylor, E Callaway, T Euler, AD Huberman
    J. Neurosci., 2018-10-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  116. Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells
    Authors: J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani
    PLoS ONE, 2018-10-29;13(10):e0206756.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  117. Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration
    Authors: HA Himburg, CM Termini, L Schlussel, J Kan, M Li, L Zhao, T Fang, JP Sasine, VY Chang, JP Chute
    Cell Stem Cell, 2018-08-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  118. Combinatorial genetics in liver repopulation and carcinogenesis with a novel in vivo CRISPR activation platform
    Authors: Kirk J. Wangensteen, Yue J. Wang, Zhixun Dou, Amber W. Wang, Elham Mosleh‐Shirazi, Max A. Horlbeck et al.
    Hepatology
  119. Leptin/Osteopontin Axis Regulated Type 2T Helper Cell Response in Allergic Rhinitis with Obesity
    Authors: Q Zeng, X Luo, M Han, W Liu, H Li
    EBioMedicine, 2018-06-07;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  120. Gankyrin Promotes Tumor-Suppressor Protein Degradation to Drive Hepatocyte Proliferation
    Authors: AM D'Souza, Y Jiang, A Cast, L Valanejad, M Wright, K Lewis, M Kumbaji, S Shah, D Smithrud, R Karns, S Shin, N Timchenko
    Cell Mol Gastroenterol Hepatol, 2018-05-24;6(3):239-255.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  121. Universality of clone dynamics during tissue development
    Authors: Steffen Rulands, Fabienne Lescroart, Samira Chabab, Christopher J. Hindley, Nicole Prior, Magdalena K. Sznurkowska et al.
    Nature Physics
  122. Metabolic modulation of acetaminophen-induced hepatotoxicity by osteopontin
    Authors: Y Wen, C Wang, J Gu, C Yu, K Wang, X Sun, Y Sun, H Wu, Y Tong, Q Xia, X Kong
    Cell. Mol. Immunol., 2018-05-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  123. HMGB1 links chronic liver injury to progenitor responses and hepatocarcinogenesis
    Authors: C Hernandez, P Huebener, JP Pradere, DJ Antoine, RA Friedman, RF Schwabe
    J. Clin. Invest., 2018-05-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  124. Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
    Authors: KH Kim, SH Kim, DH Han, YS Jo, YH Lee, MS Lee
    Sci Rep, 2018-05-01;8(1):6789.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  125. Tbr1 instructs laminar patterning of retinal ganglion cell dendrites
    Authors: J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes
    Nat. Neurosci., 2018-04-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  126. Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits
    Authors: W Ding, K Yousefi, S Goncalves, BJ Goldstein, AL Sabater, A Kloosterbo, P Ritter, G Lambert, AJ Mendez, LA Shehadeh
    JCI Insight, 2018-03-22;3(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  127. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis
    Authors: T Kobori, S Hamasaki, A Kitaura, Y Yamazaki, T Nishinaka, A Niwa, S Nakao, H Wake, S Mori, T Yoshino, M Nishibori, H Takahashi
    Front Immunol, 2018-03-06;9(0):334.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot
  128. Cardiac macrophages promote diastolic dysfunction
    Authors: M Hulsmans, HB Sager, JD Roh, M Valero-Muñ, NE Houstis, Y Iwamoto, Y Sun, RM Wilson, G Wojtkiewic, B Tricot, MT Osborne, J Hung, C Vinegoni, K Naxerova, DE Sosnovik, MR Zile, AD Bradshaw, R Liao, A Tawakol, R Weissleder, A Rosenzweig, FK Swirski, F Sam, M Nahrendorf
    J. Exp. Med., 2018-01-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  129. Type 2 diabetes impairs the ability of skeletal muscle pericytes to augment postischemic neovascularization in db/db mice
    Authors: KL Hayes, LM Messina, LM Schwartz, J Yan, AS Burnside, S Witkowski
    Am. J. Physiol., Cell Physiol., 2018-01-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  130. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  131. PEDF expression affects retinal endothelial cell proangiogenic properties through alterations in cell adhesive mechanisms
    Authors: Juliana Falero-Perez, SunYoung Park, Christine M. Sorenson, Nader Sheibani
    American Journal of Physiology-Cell Physiology
  132. 3D Visualization of Individual Regenerating Retinal Ganglion Cell Axons Reveals Surprisingly Complex Growth Paths
    Authors: Eric R. Bray, Markus Noga, Kinjal Thakor, Yunfang Wang, Vance P. Lemmon, Kevin K. Park et al.
    eNeuro
  133. Four alpha ganglion cell types in mouse retina: Function, structure, and molecular signatures
    Authors: B Krieger, M Qiao, DL Rousso, JR Sanes, M Meister
    PLoS ONE, 2017-07-28;12(7):e0180091.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  134. Bone marrow-derived monocyte infusion improves hepatic fibrosis by decreasing osteopontin, TGF-?1, IL-13 and oxidative stress
    Authors: VCA de Souza, TA Pereira, VW Teixeira, H Carvalho, MCAB de Castro, CG D'assunção, AF de Barros, CL Carvalho, VMB de Lorena, VMA Costa, ÁAC Teixeira, RCBQ Figueiredo, SA de Oliveir
    World J. Gastroenterol., 2017-07-28;23(28):5146-5157.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  135. Sox11 Expression Promotes Regeneration of Some Retinal Ganglion Cell Types but Kills Others
    Authors: MW Norsworthy, F Bei, R Kawaguchi, Q Wang, NM Tran, Y Li, B Brommer, Y Zhang, C Wang, JR Sanes, G Coppola, Z He
    Neuron, 2017-06-21;94(6):1112-1120.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  136. Monocyte Chemotactic Protein-1-Interleukin-6-Osteopontin Pathway of Intra-Aneurysmal Tissue Healing
    Authors: K Hosaka, K Rojas, HZ Fazal, MB Schneider, J Shores, V Federico, M McCord, L Lin, B Hoh
    Stroke, 2017-03-14;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  137. Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells
    Authors: N Guidi, M Sacma, L Ständker, K Soller, G Marka, K Eiwen, JM Weiss, F Kirchhoff, T Weil, JA Cancelas, MC Florian, H Geiger
    EMBO J, 2017-03-02;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  138. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017-02-20;19(3):189-201.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  139. Dentin Sialoprotein is a Novel Substrate of Matrix Metalloproteinase 9 in vitro and in vivo
    Authors: G Yuan, L Chen, J Feng, G Yang, Q Ni, X Xu, C Wan, M Lindsey, KJ Donly, M MacDougall, Z Chen, S Chen
    Sci Rep, 2017-02-14;7(0):42449.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  140. Oligo-fucoidan prevents renal tubulointerstitial fibrosis by inhibiting the CD44 signal pathway
    Authors: CH Chen, YM Sue, CY Cheng, YC Chen, CT Liu, YH Hsu, PA Hwang, NJ Huang, TH Chen
    Sci Rep, 2017-01-18;7(0):40183.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  141. Osteopontin Is a Blood Biomarker for Microglial Activation and Brain Injury in Experimental Hypoxic-Ischemic Encephalopathy
    Authors: Yikun Li, Eric B. Dammer, Xiaohui Zhang-Brotzge, Scott Chen, Duc M. Duong, Nicholas T. Seyfried et al.
    eNeuro
  142. Osteopontin Blockade Attenuates Renal Injury After Ischemia Reperfusion by Inhibiting NK Cell Infiltration
    Authors: Cindy Cen, Monowar Aziz, Weng-Lang Yang, Jeffrey M. Nicastro, Gene F. Coppa, Ping Wang
    Shock
  143. Disease-modifying effects of orally bioavailable NF-?B inhibitors in dystrophin-deficient muscle
    JCI Insight, 2016-12-22;1(21):e90341.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  144. Osteopontin regulates proliferation, apoptosis, and migration of murine claudin-low mammary tumor cells
    Authors: S. Saleh, D. E. Thompson, J. McConkey, P. Murray, R. A. Moorehead
    BMC Cancer
  145. Neutralization of Osteopontin Ameliorates Acute Lung Injury Induced by Intestinal Ischemia-Reperfusion
    Authors: Yohei Hirano, Monowar Aziz, Weng-Lang Yang, Mahendar Ochani, Ping Wang
    Shock
  146. Vav1 Regulates Mesenchymal Stem Cell Differentiation Decision Between Adipocyte and Chondrocyte via Sirt1
    Authors: Peng Qu, Lizhen Wang, Yongfen Min, Lois McKennett, Jonathan R. Keller, P. Charles Lin
    Stem Cells
  147. Selective Expression of Osteopontin in ALS-resistant Motor Neurons is a Critical Determinant of Late Phase Neurodegeneration Mediated by Matrix Metalloproteinase-9
    Authors: Yuta Morisaki
    Sci Rep, 2016-06-06;6(0):27354.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  148. The P2X4 receptor is required for neuroprotection via ischemic preconditioning
    Sci Rep, 2016-05-13;6(0):25893.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  149. High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6
    Authors: Guang-Liang Chen, Yubin Luo, Daniel Eriksson, Xianyi Meng, Cheng Qian, Tobias Bäuerle et al.
    Oncotarget
  150. Alternatively activated macrophages determine repair of the infarcted adult murine heart
    J Clin Invest, 2016-05-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  151. Osteopontin protects against high phosphate-induced nephrocalcinosis and vascular calcification
    Authors: Neil J. Paloian, Elizabeth M. Leaf, Cecilia M. Giachelli
    Kidney International
  152. Osteopontin ablation ameliorates muscular dystrophy by shifting macrophages to a pro-regenerative phenotype
    Authors: Joana Capote, Irina Kramerova, Leonel Martinez, Sylvia Vetrone, Elisabeth R. Barton, H. Lee Sweeney et al.
    Journal of Cell Biology
  153. Inhibition of hedgehog signaling ameliorates hepatic inflammation in mice with nonalcoholic fatty liver disease (NAFLD)
    Authors: Hyunjoo Kwon, Kyoungsub Song, Chang Han, Weina Chen, Ying Wang, Srikanta Dash et al.
    Hepatology
  154. The transcription factor c-JUN/AP-1 promotes HBV-related liver tumorigenesis in mice
    Authors: C Trierweiler, B Hockenjos, K Zatloukal, R Thimme, H E Blum, E F Wagner et al.
    Cell Death & Differentiation
  155. Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGF beta, and extracellular matrix
    Authors: Kerim B. Kaylan, Viktoriya Ermilova, Ravi Chandra Yada, Gregory H. Underhill
    Scientific Reports
  156. Intracellular osteopontin stabilizes TRAF3 to positively regulate innate antiviral response
    Authors: K Zhao, M Zhang, L Zhang, P Wang, G Song, B Liu, H Wu, Z Yin, C Gao
    Sci Rep, 2016-03-30;6(0):23771.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Neutralization
  157. Slc20a2 deficiency results in fetal growth restriction and placental calcification associated with thickened basement membranes and novel CD13 and laminin alpha 1 expressing cells
    Authors: Mary C. Wallingford, Hilary S. Gammill, Cecilia M. Giachelli
    Reproductive Biology
  158. Pannexin 3 and connexin 43 modulate skeletal development through their distinct functions and expression patterns
    Authors: Masaki Ishikawa, Geneva L. Williams, Tomoko Ikeuchi, Kiyoshi Sakai, Satoshi Fukumoto, Yoshihiko Yamada
    Journal of Cell Science
  159. Extracellular Adenosine Production by ecto-5'-Nucleotidase (CD73) Enhances Radiation-Induced Lung Fibrosis
    Authors: F Wirsdörfer, S de Leve, F Cappuccini, T Eldh, AV Meyer, E Gau, LF Thompson, NY Chen, H Karmouty-Q, U Fischer, M Kasper, D Klein, JW Ritchey, MR Blackburn, AM Westendorf, M Stuschke, V Jendrossek
    Cancer Res., 2016-02-26;76(10):3045-56.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  160. Inhibition of osteoblast mineralization by phosphorylated phage-derived apatite-specific peptide
    Authors: Janani Ramaswamy, Hwa Kyung Nam, Harsha Ramaraju, Nan E. Hatch, David H. Kohn
    Biomaterials
  161. Neutralization of osteopontin attenuates neutrophil migration in sepsis-induced acute lung injury
    Authors: Yohei Hirano, Monowar Aziz, Weng-Lang Yang, Zhimin Wang, Mian Zhou, Mahendar Ochani et al.
    Critical Care
  162. Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.
    Authors: Mu X, Espanol-Suner R, Mederacke I, Affo S, Manco R, Sempoux C, Lemaigre F, Adili A, Yuan D, Weber A, Unger K, Heikenwalder M, Leclercq I, Schwabe R
    J Clin Invest, 2015-09-08;125(10):3891-903.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  163. Cutting edge: Role of osteopontin and integrin alphav in T cell-mediated anti-inflammatory responses in endotoxemia.
    Authors: Inoue M, Shinohara M
    J Immunol, 2015-05-13;194(12):5595-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates, In Vivo
    Applications: ELISA Development (Capture), Neutralization
  164. Identification of therapeutic targets for glioblastoma by network analysis.
    Authors: Friedmann-Morvinski D, Bhargava V, Gupta S, Verma I, Subramaniam S
    Oncogene, 2015-05-11;35(5):608-20.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  165. Osteopontin depletion decreases inflammation and gastric epithelial proliferation during Helicobacter pylori infection in mice.
    Authors: Park J, Lee S, Go D, Kim H, Kwon H, Kim D
    Lab Invest, 2015-04-13;95(6):660-71.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  166. Loss of JUNB/AP-1 promotes invasive prostate cancer.
    Authors: Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E
    Cell Death Differ, 2014-12-19;22(4):574-82.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  167. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2014-11-24;18(1):145-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  168. PGC-1alpha induces SPP1 to activate macrophages and orchestrate functional angiogenesis in skeletal muscle.
    Authors: Rowe G, Raghuram S, Jang C, Nagy J, Patten I, Goyal A, Chan M, Liu L, Jiang A, Spokes K, Beeler D, Dvorak H, Aird W, Arany Z
    Circ Res, 2014-07-09;115(5):504-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  169. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014-03-12;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  170. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
    Authors: Kusumbe A, Ramasamy S, Adams R
    Nature, 2014-03-12;507(7492):323-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  171. Colony-stimulating factor-1 signaling suppresses renal crystal formation.
    Authors: Taguchi K, Okada A, Kitamura H, Yasui T, Naiki T, Hamamoto S, Ando R, Mizuno K, Kawai N, Tozawa K, Asano K, Tanaka M, Miyoshi I, Kohri K
    J Am Soc Nephrol, 2014-02-27;25(8):1680-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  172. Osteopontin expression by CD103- dendritic cells drives intestinal inflammation.
    Authors: Kourepini E, Aggelakopoulou M, Alissafi T, Paschalidis N, Simoes D, Panoutsakopoulou V
    Proc Natl Acad Sci U S A, 2014-02-18;111(9):E856-65.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  173. An osteopontin-integrin interaction plays a critical role in directing adipogenesis and osteogenesis by mesenchymal stem cells.
    Authors: Chen Q, Shou P, Zhang L, Xu C, Zheng C, Han Y, Li W, Huang Y, Zhang X, Shao C, Roberts A, Rabson A, Ren G, Zhang Y, Wang Y, Denhardt D, Shi Y
    Stem Cells, 2014-02-01;32(2):327-37.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  174. Osteopontin is an initial mediator of inflammation and liver injury during obstructive cholestasis after bile duct ligation in mice
    Authors: Min Yang, Anup Ramachandran, Hui-Min Yan, Benjamin L. Woolbright, Bryan L. Copple, Peter Fickert et al.
    Toxicology Letters
  175. Hydrogen peroxide regulates osteopontin expression through activation of transcriptional and translational pathways.
    Authors: Lyle A, Remus E, Fan A, Lassegue B, Walter G, Kiyosue A, Griendling K, Taylor W
    J Biol Chem, 2013-11-18;289(1):275-85.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  176. Impaired hepatocyte maturation, abnormal expression of biliary transcription factors and liver fibrosis in C/EBPalpha(Cebpa)-knockout mice.
    Authors: Akai Y, Oitate T, Koike T, Shiojiri N
    Histol Histopathol, 2013-07-18;29(1):107-25.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  177. Endoglin regulates the activation and quiescence of endothelium by participating in canonical and non-canonical TGF-beta signaling pathways.
    Authors: Park S, Dimaio T, Liu W, Wang S, Sorenson C, Sheibani N
    J Cell Sci, 2013-02-15;126(0):1392-405.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  178. Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments.
    Authors: Park S, Wolfram P, Canty K, Harley B, Nombela-Arrieta C, Pivarnik G, Manis J, Beggs H, Silberstein L
    J Immunol, 2012-12-21;190(3):1094-102.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  179. Identification of a clonally expanding haematopoietic compartment in bone marrow.
    Authors: Wang L, Benedito R, Bixel M, Zeuschner D, Stehling M, Savendahl L, Haigh J, Snippert H, Clevers H, Breier G, Kiefer F, Adams R
    EMBO J, 2012-11-27;32(2):219-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  180. Direction-selective retinal ganglion cells arise from molecularly specified multipotential progenitors
    Authors: Irina De la Huerta, In-Jung Kim, P. Emanuela Voinescu, Joshua R. Sanes
    Proceedings of the National Academy of Sciences
  181. Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
    Authors: Lau, Wei Ling, Leaf, Elizabet, Hu, Ming Cha, Takeno, Marc M, Kuro-o, Makoto, Moe, Orson W, Giachelli, Cecilia
    Kidney Int, 2012-08-29;82(12):1261-70.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  182. Loss of MMP-2 in murine osteoblasts upregulates osteopontin and bone sialoprotein expression in a circuit regulating bone homeostasis
    Authors: Rebecca A. Mosig, John A. Martignetti
    Disease Models & Mechanisms
  183. Repression of osteocyte Wnt/β-catenin signaling is an early event in the progression of renal osteodystrophy.
    Authors: Sabbagh Y, Graciolli FG, O'Brien S, Tang W, Dos Reis LM, Ryan S, Phillips L, Boulanger J, Song W, Bracken C, Liu S, Ledbetter S, Dechow P, Canziani ME, Carvalho AB, Jorgetti V, Moyses RM, Schiavi SC
    J. Bone Miner. Res., 2012-08-01;27(8):1757-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  184. Cellular basis of tissue regeneration by omentum.
    Authors: Shah S, Lowery E, Braun RK
    PLoS ONE, 2012-06-06;7(6):e38368.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  185. Neuronal Classification and Marker Gene Identification via Single-Cell Expression Profiling of Brainstem Vestibular Neurons Subserving Cerebellar Learning
    Authors: Takashi Kodama, Shiloh Guerrero, Minyoung Shin, Seti Moghadam, Michael Faulstich, Sascha du Lac
    Journal of Neuroscience
  186. Proteomic Characterization of the Cellular Response to Nitrosative Stress Mediated by S-Nitrosoglutathione Reductase Inhibition
    Authors: Matthew W. Foster, Zhonghui Yang, David M. Gooden, J. Will Thompson, Carol H. Ball, Meredith E. Turner et al.
    Journal of Proteome Research
  187. The critical role of agrin in the hematopoietic stem cell niche.
    Authors: Mazzon C, Anselmo A, Cibella J, Soldani C, Destro A, Kim N, Roncalli M, Burden SJ, Dustin ML, Sarukhan A, Viola A
    Blood, 2011-06-07;118(10):2733-42.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  188. Suppressed monocyte recruitment drives macrophage removal from atherosclerotic plaques of Apoe–/– mice during disease regression
    Authors: Stephane Potteaux, Emmanuel L. Gautier, Susan B. Hutchison, Nico van Rooijen, Daniel J. Rader, Michael J. Thomas et al.
    Journal of Clinical Investigation
  189. Cutting Edge: Critical Role of Intracellular Osteopontin in Antifungal Innate Immune Responses
    Authors: Makoto Inoue, Yasuhiro Moriwaki, Tomohiro Arikawa, Yu-Hsun Chen, Young Joo Oh, Timothy Oliver et al.
    The Journal of Immunology
  190. T-Lymphocyte Responses to Intestinally Absorbed Antigens Can Contribute to Adipose Tissue Inflammation and Glucose Intolerance during High Fat Feeding
    Authors: Yuehui Wang, Jianing Li, Lihua Tang, Yu Wang, Richard Charnigo, Willem de Villiers et al.
    PLoS ONE
  191. Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance.
    Authors: Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Saemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM
    Diabetes, 2010-01-27;59(4):935-46.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  192. Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
    Authors: Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR
    FASEB J., 2009-08-31;24(1):70-80.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  193. Therapeutic silencing of an endogenous gene by siRNA cream in an arthritis model mouse.
    Authors: Takanashi M, Oikawa K, Sudo K, Tanaka M, Fujita K, Ishikawa A, Nakae S, Kaspar RL, Matsuzaki M, Kudo M, Kuroda M
    Gene Ther., 2009-05-28;16(8):982-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  194. Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin.
    Authors: El-Abbadi MM, Pai AS, Leaf EM, Yang HY, Bartley BA, Quan KK, Ingalls CM, Liao HW, Giachelli CM
    Kidney Int., 2009-03-25;75(12):1297-307.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  195. Increased osteopontin expression in dendritic cells amplifies IL-17 production by CD4+ T cells in experimental autoimmune encephalomyelitis and in multiple sclerosis.
    Authors: Murugaiyan G, Mittal A, Weiner HL
    J. Immunol., 2008-12-01;181(11):7480-8.
    Species: Mouse
    Sample Types: Cell Lysates, In Vivo
    Applications: Neutralization, Western Blot
  196. Fibroblast growth factor-2 regulates expression of osteopontin in periodontal ligament cells.
    Authors: Terashima Y, Shimabukuro Y, Terashima H, Ozasa M, Terakura M, Ikezawa K, Hashikawa T, Takedachi M, Oohara H, Yamada S, Murakami S
    J. Cell. Physiol., 2008-09-01;216(3):640-50.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  197. In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression.
    Authors: Marcondes MC, Poling M, Watry DD, Hall D, Fox HS
    Cell. Immunol., 2008-08-03;254(1):56-62.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot
  198. Polyomavirus middle T antigen induces the transcription of osteopontin, a gene important for the migration of transformed cells.
    Authors: Whalen KA, Weber GF, Benjamin TL, Schaffhausen BS
    J. Virol., 2008-03-12;82(10):4946-54.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  199. Osteopontin has a crucial role in allergic airway disease through regulation of dendritic cell subsets.
    Authors: Xanthou G, Alissafi T, Semitekolou M, Simoes DC, Economidou E, Gaga M, Lambrecht BN, Lloyd CM, Panoutsakopoulou V
    Nat. Med., 2007-04-15;13(5):570-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  200. Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
    Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
    Eur. Respir. J., 2006-04-26;28(2):275-85.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  201. Aggregation of embryonic stem cells induces Nanog repression and primitive endoderm differentiation.
    Authors: Hamazaki T, Oka M, Yamanaka S, Terada N
    J. Cell. Sci., 2004-10-19;117(0):5681-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  202. Lack of requirement of osteopontin for inflammation, bone erosion, and cartilage damage in the K/BxN model of autoantibody-mediated arthritis.
    Authors: Jacobs JP, Pettit AR, Shinohara ML, Jansson M, Cantor H, Gravallese EM, Mathis D, Benoist C
    Arthritis Rheum., 2004-08-01;50(8):2685-94.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  203. Inactivation of the osteopontin gene enhances vascular calcification of matrix Gla protein-deficient mice: evidence for osteopontin as an inducible inhibitor of vascular calcification in vivo
    Authors: Mei Y. Speer, Marc D. McKee, Robert E. Guldberg, Lucy Liaw, Hsueh-Ying Yang, Elyse Tung et al.
    The Journal of Experimental Medicine
  204. Retinal patterns and the cellular repertoire of neuropsin (Opn5) retinal ganglion cells
    Authors: D'Souza SP, Swygart DI, Wienbar SR Et al.
    The Journal of comparative neurology
  205. Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae
    Authors: Jiang D, Burger CA, Casasent AK et al.
    J. Comp. Neurol.
  206. YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells
    Authors: Sivaraj KK, Dharmalingam B, Mohanakrishnan V et al.
    Elife
  207. PEDF Expression Affects the Oxidative and Inflammatory State of Choroidal Endothelial Cells
    Authors: M Farnoodian, CM Sorenson, N Sheibani
    Am. J. Physiol., Cell Physiol., 2018-01-10;0(0):.

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Reviews for Mouse Osteopontin/OPN Antibody

Average Rating: 4.1 (Based on 11 Reviews)

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Mouse Osteopontin/OPN Antibody
By Anonymous on 10/03/2024
Application: IHC
Reason for Rating: The antibody works excellently. It labels Spp1 on microglia in 5xFAD mouse model of Alzheimer's disease Sample Tested: Alzheimer's disease brain Species: Mouse
Immunohistochemistry Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 05/02/2024
Application: Immunocytochemistry/Immunofluorescence Sample Tested: C2C12 mouse myoblast cell line Species: Mouse

BSA 4% as blocking solution. Negative control with secondary only confirmed that the staining is specific.

Immunocytochemistry/Immunofluorescence Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 02/13/2024
Application: IHC Sample Tested: Skeletal muscle section,Skeletal muscle tissue Species: Mouse

No positive IF signal in fresh frozen skeletal muscle section.

Immunohistochemistry Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 09/18/2022
Application: Flow Sample Tested: Lung cancer tissue Species: Mouse

Try it for flow cytometry

Flow Cytometry Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 07/14/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult pancreas Species: Mouse

Osteopontin (in white) was stained 1:200 on cryostat sections together with CD324-AF488 (in green). Ducts are clearly stained as expected.

Immunocytochemistry/Immunofluorescence Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Loïc Joannes on 07/02/2021
Application: WB Sample Tested: Cancer cell lysates,Cell Culture Media Species: Mouse

Antibody reacts with BSA and some of the ladder proteins from Biorad. Unusable for WB since BSA size is too close from the OSTP size.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.*** Update from Tech Support Team: Possible customer error, no response from customer.***

Western Blot Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 11/22/2020
Application: Func Sample Tested: Plasma Species: Mouse
Functional Assay Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 06/17/2020
Application: IHC Sample Tested: Liver tissue Species: Mouse
Immunohistochemistry Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Mingxia Zhou on 02/16/2020
Application: WB Sample Tested: A20 mouse B cell lymphoma cell line Species: Mouse
Western Blot Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Christian Schuerch on 08/31/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: bone marrow Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse Osteopontin/OPN Antibody AF808
Mouse Osteopontin/OPN Antibody
By Anonymous on 04/26/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Cell Culture Samples Species: Rat