Mouse Netrin-1 Antibody Summary
Val22-Ala603
Accession # AAC52971
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Netrin-1 by Western Blot UNC5A and UNC5C induce caspase-3 activation through DAPK1/PR65b during UPR. (A) Quantification of netrin-1–receptor expression by qRT-PCR (mean + SEM, n = 3). (B–F) Identification of the implicated netrin-1 receptors. HepaRG cells were transfected with siRNAs and treated with DTT or not for 4 hours (mock). (B) Assessment of XBP1 mRNA splicing by RT-PCR. Representative result, n = 3. (C) Assessment of netrin-1 protein knockdown by immunoblotting. Representative result, n = 3. (D and E) Assessment of transcript knockdown efficiencies. Graphs indicate (D) UNC5A and (E) UNC5C mRNA levels in siRNA-treated cells in comparison with control siRNA-treated cells (mean + SEM; n = 3; Mann–Whitney test; P <.05). (F) Caspase-3 activation is reversed by UNC5A or UNC5C knockdown after UPR induction. Graph indicates caspase-3 activity ratio of DTT vs untreated cells for each condition (mean + SEM; n = 3; Mann–Whitney test; P <.05). (G–J) Identification of the downstream signaling pathway. HepaRG cells were transfected with siRNAs and treated with DTT or not for 4 hours (mock). (G) Assessment of XBP1 mRNA splicing by RT-PCR. Representative result, n = 3. (H) Evaluation of netrin-1, DAPK1, and PR65 beta depletion by immunoblotting. Representative result, n = 3. (I) Caspase-3 activation is reversed by DAPK1 or PR65 beta knockdown. Graph indicates the caspase-3 activity ratio for each condition (mean + SEM; n = 3; Mann–Whitney test; P <.05). (J) PP2A activity is increased by netrin-1 depletion and reversed by reduced expression of PR65 beta. Graph indicates PP2A activity ratio for each condition (mean + SEM; n = 3; Mann–Whitney test; P <.05). *,**, or *** refer to statistical analyses. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28174720), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Netrin-1
Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma -chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin‑1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis,
Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1-7).
- Puschel, A. (1999) Mech. Dev. 83:65.
- Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
- Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
- Madison, R. et al. (2000) Exp. Neurology 161:563.
- Srinivasan, K. et al. (2003) Dev. Cell 4:371.
- Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
- Corset, V. et al. (2000) Nature 407:747.
Product Datasheets
Citations for Mouse Netrin-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Cannabinoid Receptor CB2 Modulates Axon Guidance
Authors: Gabriel Duff, Anteneh Argaw, Bruno Cecyre, Hosni Cherif, Nicolas Tea, Nawal Zabouri et al.
PLoS ONE
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Netrin 1-Mediated Role of the Substantia Nigra Pars Compacta and Ventral Tegmental Area in the Guidance of the Medial Habenular Axons
Authors: Verónica Company, Abraham Andreu-Cervera, M. Pilar Madrigal, Belén Andrés, Francisca Almagro-García, Alain Chédotal et al.
Frontiers in Cell and Developmental Biology
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Allosteric potentiation of GABAA receptor single-channel conductance by netrin-1 during neuronal-excitation-induced inhibitory synaptic homeostasis
Authors: ES Chan, Y Ge, YW So, YF Bai, L Liu, YT Wang
Cell Reports, 2022-11-01;41(5):111584.
Species: Mouse
Sample Types: Whole Cells
Applications: Immunodepletion -
Implication of Netrin-1 Gain of Expression in Canine Nodal Lymphoma
Authors: A Tortereau, N Milhau, E Rhumy, M Castets, F Ponce, P Mehlen, T Marchal
Veterinary sciences, 2022-09-10;9(9):.
Species: Canine
Sample Types: Whole Cells, Whole Tissue
Applications: FLOW, Flow Cytometry, IHC -
Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury
Authors: JT Chen, L Schmidt, C Schürger, MK Hankir, SM Krug, HL Rittner
International Journal of Molecular Sciences, 2021-09-18;22(18):.
Species: Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity
Authors: P Mehrpouya-, K Miranda, NP Singh, EE Zumbrun, M Nagarkatti, PS Nagarkatti
J. Biol. Chem., 2019-03-25;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Mutations in the netrin-1 gene cause congenital mirror movements
Authors: A Méneret, EA Franz, O Trouillard, TC Oliver, Y Zagar, SP Robertson, Q Welniarz, RJM Gardner, C Gallea, M Srour, C Depienne, CL Jasoni, C Dubacq, F Riant, JC Lamy, MP Morel, R Guérois, J Andreani, C Fouquet, M Doulazmi, M Vidailhet, GA Rouleau, A Brice, A Chédotal, I Dusart, E Roze, D Markie
J. Clin. Invest., 2017-09-25;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Floor-plate-derived netrin-1 is dispensable for commissural axon guidance
Authors: C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal
Nature, 2017-04-26;0(0):.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC, Western Blot -
Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response
Authors: T Lahlali, ML Plissonnie, C Romero-Lóp, M Michelet, B Ducarouge, A Berzal-Her, F Zoulim, P Mehlen, R Parent
Cell Mol Gastroenterol Hepatol, 2016-01-09;2(3):281-301.e9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
Nat Med, 2014-03-02;20(4):377-84.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Neuronal guidance protein Netrin-1 induces differentiation in human embryonal carcinoma cells.
Authors: Mancino M, Esposito C, Watanabe K, Nagaoka T, Gonzales M, Bianco C, Normanno N, Salomon DS, Strizzi L
Cancer Res., 2009-02-17;69(5):1717-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Netrin-1 interacts with amyloid precursor protein and regulates amyloid-beta production.
Authors: Lourenco FC, Galvan V, Fombonne J, Corset V, Llambi F, Muller U, Bredesen DE, Mehlen P
Cell Death Differ., 2009-01-16;16(5):655-63.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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Immunohistochemistry worked nicely with this antibody. I performed staining in transverse sections of C57BL/6 mouse DRGs, Spinal cord sections and longitudinal sections of sciatic nerve. I would recommend doing antigen retrieval using sodium citrate buffer at pH 6.0 at a temperature of 80-85C for 30 min in a hot water bath. For all mentioned tissues I PFA fixed it overnight then cut small sections of the spinal cord and cut the sciatic nerve into two (longitudinally), antigen retrieved them in a 12-well plate with wells filled with sodium citrate buffer and kept in a pre-heated water bath. Following this I left the samples in 30% sucrose for two nights at 4C and then embedded it in OCT followed by cryosectioning and normal IHC protocol. Worked beautifully for my samples following this protocol.
