Summary
Precision
Recovery
Linearity
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Mouse MSP/MST1 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
Sensitivity
0.019 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse MSP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse MSP/MST1 Immunoassay is a 4.5 hour solid phase ELISA
designed to measure mouse MSP in cell culture supernates, tissue lysates,
serum, plasma, and urine. It contains CHO cell-expressed recombinant mouse MSP
and antibodies raised against the recombinant factor. This immunoassay
has been shown to accurately quantitate recombinant mouse MSP.
Results obtained using natural mouse MSP showed linear curves that
were parallel to the standard curves obtained using the Quantikine kit
standards. These results indicate that this kit can be used to
determine relative mass values for natural mouse MSP.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays
to determine inter-assay precision. Assays were performed by at least
three technicians using two lots of kit components
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.504 | 1.28 | 3.98 | 0.47 | 1.24 | 3.74 |
| Standard Deviation | 0.027 | 0.068 | 0.154 | 0.015 | 0.044 | 0.228 |
| CV% | 5.4 | 5.3 | 3.9 | 3.2 | 3.6 | 6.1 |
Recovery
The recovery of mouse MSP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 98 | 90-108 |
| Tissue Homogenates (n=4) | 101 | 90-109 |
| Urine (n=4) | 96 | 88-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked
with high concentrations of mouse MSP were serially diluted with the
appropriate Calibrator Diluent to produce samples with values within
the dynamic range of the assay.
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MSP/MST1
Macrophage-stimulating protein (MSP), also known as Hepatocyte Growth Factor-like Protein (HGFL) and Scatter Factor-2 (SF2), is a member of the HGF family of growth factors. MSP is secreted as a single chain inactive precursor (pro-MSP). In response to injury, pro-MSP is activated by proteolytic cleavage. Activation results in the creation of alpha and beta chains joined by a disulfide bond.
Long Name:
Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]
Entrez Gene IDs:
4485 (Human); 15235 (Mouse); 24566 (Rat)
Alternate Names:
D3F15S2; EC 3.4.21; hepatocyte growth factor-like protein homolog; HGFL; HGFLhepatocyte growth factor-like protein; macrophage stimulating 1 (hepatocyte growth factor-like); Macrophage stimulatory protein; Macrophage-stimulating protein; MSP; MSPDNF15S2; MST1; NF15S2; SF2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
FAQs
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