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Mouse M-CSF Quantikine ELISA Kit

Catalog #: MMC00B
33 Citations Datasheet / COA / SDS
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MMC00B
Mouse M-CSF Quantikine Spiked Recovery Competitor Comparison
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Citations (33)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse M-CSF Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.486 pg/mL
Assay Range
6.3 - 400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse M-CSF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine™ Mouse M-CSF Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse M-CSF levels in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant mouse M-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse M-CSF. Results obtained using natural mouse M-CSF showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards.  These results indicate that this kit can be used to determine relative mass values for natural mouse M-CSF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 218 213 57.8 23 224 11.2
Standard Deviation 1.05 9.97 1.72 1.61 14.8 0.765
CV% 4.8 4.7 3 7 6.6 6.8

Recovery

The recovery of mouse M-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 97-108
EDTA Plasma (n=4) 97 92-105
Heparin Plasma (n=4) 99 93-110
Serum (n=4) 94 88-98

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse M-CSF in each matrix were diluted with calibrator diluent and assayed.
Mouse M-CSF ELISA Linearity

Scientific Data

ELISA View Larger

Mouse M-CSF Quantikine Spiked Recovery Competitor Comparison M-CSF is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Plasma recovery is 103% compared to 84% for the top competitor. Cell culture supernates recovery is 100% compared to 75% for the top competitor.

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Mouse M-CSF ELISA Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Long Name:
Macrophage Colony Stimulating Factor
Entrez Gene IDs:
1435 (Human); 12977 (Mouse)
Alternate Names:
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930

Citations for Mouse M-CSF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. The circadian regulator PER1 promotes cell reprogramming by inhibiting inflammatory signaling from macrophages
    Authors: Katoku-Kikyo, N;Lim, S;Yuan, C;Koroth, J;Nakagawa, Y;Bradley, EW;Kikyo, N;
    PLoS biology
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Transitional premonocytes emerge in the periphery for host defense against bacterial infections
    Authors: YC Teh, MY Chooi, D Liu, I Kwok, GC Lai, L Ayub Ow Yo, M Ng, JLY Li, Y Tan, M Evrard, L Tan, KH Liong, K Leong, CC Goh, AYJ Chan, NB Shadan, CK Mantri, YY Hwang, H Cheng, T Cheng, W Yu, HL Tey, A Larbi, A St John, V Angeli, C Ruedl, B Lee, F Ginhoux, SL Chen, LG Ng, JL Ding, SZ Chong
    Science Advances, 2022-03-04;8(9):eabj4641.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  3. M-CSF supports medullary erythropoiesis and erythroid iron demand following burn injury through its activity on homeostatic iron recycling
    Authors: JG Noel, SW Ramser, L Pitstick, JP Bonamer, B Mackenzie, KG Seu, TA Kalfa, JA Cancelas, JC Gardner
    Scientific Reports, 2022-01-24;12(1):1235.
    Species: Mouse
    Sample Types: Serum
  4. Elevated expression of the colony-stimulating factor 1 (CSF1) induces prostatic intraepithelial neoplasia dependent of epithelial-Gp130
    Authors: OJ Kwon, B Zhang, D Jia, L Zhang, X Wei, Z Zhou, D Liu, KT Huynh, K Zhang, Y Zhang, P Labhart, A Sboner, C Barbieri, MC Haffner, CJ Creighton, L Xin
    Oncogene, 2022-01-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Biomimetic nanoparticles deliver mRNAs encoding costimulatory receptors and enhance T cell mediated cancer immunotherapy
    Authors: W Li, X Zhang, C Zhang, J Yan, X Hou, S Du, C Zeng, W Zhao, B Deng, DW McComb, Y Zhang, DD Kang, J Li, WE Carson, Y Dong
    Nature Communications, 2021-12-14;12(1):7264.
    Species: Mouse
    Sample Types: Plasma
  6. Hepatocyte-specific glucose-6-phosphatase deficiency disturbs platelet aggregation and decreases blood monocytes upon fasting-induced hypoglycemia
    Authors: AM La Rose, V Bazioti, JA Hoogerland, AF Svendsen, AG Groenen, M van Faasse, MGS Rutten, NJ Kloosterhu, B Dethmers-A, JH Nijland, G Mithieux, F Rajas, F Kuipers, MV Lukens, O Soehnlein, MH Oosterveer, M Westerterp
    Molecular Metabolism, 2021-06-04;53(0):101265.
    Species: Mouse
    Sample Types: Plasma
  7. Pharmacological normalization of pancreatic cancer-associated fibroblast secretome impairs pro-metastatic cross-talk with macrophages: Stromal CSF-1 facilitates metastasis
    Authors: R Samain, A Brunel, T Douché, M Fanjul, S Cassant-So, J Rochotte, J Cros, C Neuzillet, J Raffenne, C Duluc, A Perraud, J Nigri, V Gigoux, I Bieche, M Ponzo, G Carpentier, I Cascone, R Tomasini, HA Schmid, M Mathonnet, R Nicolle, MP Bousquet, Y Martineau, S Pyronnet, C Jean, C Bousquet
    Cellular and Molecular Gastroenterology and Hepatology, 2021-01-20;0(0):.
    Species: Mouse
    Sample Types: Plasma
  8. Tumor derived UBR5 promotes ovarian cancer growth and metastasis through inducing immunosuppressive macrophages
    Authors: M Song, OO Yeku, S Rafiq, T Purdon, X Dong, L Zhu, T Zhang, H Wang, Z Yu, J Mai, H Shen, B Nixon, M Li, RJ Brentjens, X Ma
    Nature Communications, 2020-12-08;11(1):6298.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. CSF1R signaling is a regulator of pathogenesis in progressive MS
    Authors: N Hagan, JL Kane, D Grover, L Woodworth, C Madore, J Saleh, J Sancho, J Liu, Y Li, J Proto, M Zelic, A Mahan, M Kothe, AA Scholte, M Fitzgerald, B Gisevius, A Haghikia, O Butovsky, D Ofengeim
    Cell Death Dis, 2020-10-23;11(10):904.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Effective hematopoietic stem cell-based gene therapy in a murine model of hereditary pulmonary alveolar proteinosis
    Authors: M Hetzel, E Lopez-Rodr, A Mucci, AHH Nguyen, T Suzuki, K Shima, T Buchegger, S Dettmer, T Rodt, JP Bankstahl, P Malik, L Knudsen, A Schambach, G Hansen, BC Trapnell, N Lachmann, T Moritz
    Haematologica, 2019-07-09;105(4):1147-1157.
    Species: Mouse
    Sample Types: Bronchoalveolar lavage fluid
  11. Mesothelial cell CSF1 sustains peritoneal macrophage proliferation
    Authors: S Ivanov, A Gallerand, M Gros, MI Stunault, J Merlin, N Vaillant, L Yvan-Charv, RR Guinamard
    Eur. J. Immunol., 2019-07-05;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. A Selective FGFR inhibitor AZD4547 suppresses RANKL/M-CSF/OPG-dependent ostoclastogenesis and breast cancer growth in the metastatic bone microenvironment
    Authors: J Kang, YJ Choi, BY Seo, U Jo, SI Park, YH Kim, KH Park
    Sci Rep, 2019-06-19;9(1):8726.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Macrophage-derived granulin drives resistance to immune checkpoint inhibition in metastatic pancreatic cancer
    Authors: V Quaranta, C Rainer, SR Nielsen, ML Raymant, MS Ahmed, DD Engle, A Taylor, T Murray, F Campbell, DH Palmer, DA Tuveson, A Mielgo, MC Schmid
    Cancer Res., 2018-05-22;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. A Macrophage Colony-Stimulating Factor-Producing ?? T Cell Subset Prevents Malarial Parasitemic Recurrence
    Authors: MR Mamedov, A Scholzen, RV Nair, K Cumnock, JA Kenkel, JHM Oliveira, DL Trujillo, N Saligrama, Y Zhang, F Rubelt, DS Schneider, YH Chien, RW Sauerwein, MM Davis
    Immunity, 2018-02-06;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Circuit Design Features of a Stable Two-Cell System
    Authors: X Zhou, RA Franklin, M Adler, JB Jacox, W Bailis, JA Shyer, RA Flavell, A Mayo, U Alon, R Medzhitov
    Cell, 2018-02-01;172(4):744-757.e17.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling
    Authors: W Lu, D Jia, S An, M Mu, X Qiao, Y Liu, X Li, D Wang
    Sci Rep, 2017-08-21;7(1):8402.
    Species: Mouse
    Sample Types: Plasma
  17. iRhom2 regulates CSF1R cell surface expression and non-steady state myelopoiesis in mice
    Eur J Immunol, 2016-09-28;0(0):.
    Species: Mouse
    Sample Types: Serum
  18. Monocyte/macrophage lineage commitment and distribution are affected by the lack of regulatory T cells in scurfy mice
    Eur J Immunol, 2016-05-27;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
    Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
    Stem Cells Dev, 2015-02-05;24(9):1073-81.
    Species: Mouse
    Sample Types: Serum
  20. Blockade of Fas signaling in breast cancer cells suppresses tumor growth and metastasis via disruption of Fas signaling-initiated cancer-related inflammation.
    Authors: Liu Q, Tan Q, Zheng Y, Chen K, Qian C, Li N, Wang Q, Cao X
    J Biol Chem, 2014-03-13;289(16):11522-35.
    Species: Mouse
    Sample Types: Whole Cells
  21. KIT oncogene inhibition drives intratumoral macrophage M2 polarization.
    Authors: Cavnar, Michael, Zeng, Shan, Kim, Teresa S, Sorenson, Eric C, Ocuin, Lee M, Balachandran, Vinod P, Seifert, Adrian M, Greer, Jonathan, Popow, Rachel, Crawley, Megan H, Cohen, Noah A, Green, Benjamin, Rossi, Ferdinan, Besmer, Peter, Antonescu, Cristina, DeMatteo, Ronald P
    J Exp Med, 2013-12-09;210(13):2873-86.
    Species: Mouse
    Sample Types: Serum
  22. Steady-state neutrophil homeostasis is dependent on TLR4/TRIF signaling.
    Authors: Bugl S, Wirths S, Radsak M, Schild H, Stein P, Andre M, Muller M, Malenke E, Wiesner T, Marklin M, Frick J, Handgretinger R, Rammensee H, Kanz L, Kopp H
    Blood, 2012-12-05;121(5):723-33.
    Species: Mouse
    Sample Types: Plasma
  23. The combination of the histone deacetylase inhibitor vorinostat and synthetic triterpenoids reduces tumorigenesis in mouse models of cancer.
    Authors: Tran, Kim, Risingsong, Renee, Royce, Darlene, Williams, Charlott, Sporn, Michael, Pioli, Patricia, Gediya, Lalji K, Njar, Vincent, Liby, Karen T
    Carcinogenesis, 2012-10-06;34(1):199-210.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization.
    Authors: Miyamoto K, Yoshida S, Kawasumi M
    J. Exp. Med., 2011-10-17;208(11):2175-81.
    Species: Mouse
    Sample Types: Serum
  25. Temporal changes in myeloid cells in the cervix during pregnancy and parturition.
    Authors: Timmons BC, Fairhurst AM, Mahendroo MS
    J. Immunol., 2009-03-01;182(5):2700-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
    Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
    Arthritis Rheum., 2008-11-01;58(11):3510-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells.
    Authors: Islam S, Hassan F, Tumurkhuu G, Dagvadorj J, Koide N, Naiki Y, Mori I, Yoshida T, Yokochi T
    Biochem. Biophys. Res. Commun., 2007-06-12;360(2):346-51.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. IL-7 induces myelopoiesis and erythropoiesis.
    Authors: Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK
    J. Immunol., 2007-02-01;178(3):1553-63.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. Concerted action of Smad and CREB-binding protein regulates bone morphogenetic protein-2-stimulated osteoblastic colony-stimulating factor-1 expression.
    Authors: Ghosh-Choudhury N, Singha PK, Woodruff K, St Clair P, Bsoul S, Werner SL, Choudhury GG
    J. Biol. Chem., 2006-05-17;281(29):20160-70.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. Perforin-deficient CD8+ T cells mediate fatal lymphocytic choriomeningitis despite impaired cytokine production.
    Authors: Storm P, Bartholdy C, Sorensen MR, Christensen JP, Thomsen AR
    J. Virol., 2006-02-01;80(3):1222-30.
    Species: Mouse
    Sample Types: Serum
  31. The cell-surface isoform of colony stimulating factor 1 (CSF1) restores but does not completely normalize fecundity in CSF1-deficient mice.
    Authors: Ovadia S, Insogna K, Yao GQ
    Biol. Reprod., 2005-10-19;74(2):331-6.
    Species: Mouse
    Sample Types: Tissue Homogenates
  32. Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation.
    Authors: Sergejeva S, Ivanov S, Lotvall J, Linden A
    Am. J. Respir. Cell Mol. Biol., 2005-05-18;33(3):248-53.
    Species: Mouse
    Sample Types: BALF
  33. Differential roles of CC chemokine ligand 2/monocyte chemotactic protein-1 and CCR2 in the development of T1 immunity.
    Authors: Traynor TR, Herring AC, Dorf ME, Kuziel WA, Toews GB, Huffnagle GB
    J. Immunol., 2002-05-01;168(9):4659-66.
    Species: Mouse
    Sample Types: BALF

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