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Mouse LYVE-1 Biotinylated Antibody

Catalog #: BAF2125
13 Citations Datasheet / COA / SDS
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BAF2125
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Citations (13)
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Mouse LYVE-1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LYVE-1 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) LYVE-1 and less than 1% cross-reactivity with rhCD44 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LYVE-1
Ala24-Thr234
Accession # Q8BHC0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse LYVE‑1 (Catalog # 2125-LY)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse liver and spleen

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

References
  1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
  2. Evered, D. and J. Whelan (1989) Ciba Found. Symp. 143:1.
  3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
  4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
  5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
  6. Carreira, C.M. et al. (2001) Cancer Research 61:8079.
  7. Jackson, D.J. et al. (2001) Trends Immunol. 22:317.
  8. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
  9. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
  10. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
  11. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.
Long Name
Lymphatic Vessel Endothelial Hyaluronan Receptor 1
Entrez Gene IDs
10894 (Human); 114332 (Mouse); 293186 (Rat)
Alternate Names
cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; UNQ230/PRO263; XLKD1

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Citations for Mouse LYVE-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Thrombospondin 1 missense alleles induce extracellular matrix protein aggregation and TM dysfunction in congenital glaucoma
    Authors: Fu H, Siggs OM, Knight LS et al.
    The Journal of clinical investigation
  2. Micro- and Macro-Anatomical Frameworks of Lymph Nodes Indispensable for the Lymphatic System Filtering Function
    Authors: Madoka Ozawa, Shihori Nakajima, Daichi Kobayashi, Koichi Tomii, Nan-Jun Li, Tomoya Watarai et al.
    Frontiers in Cell and Developmental Biology
  3. The phosphatase PTEN links platelets with immune regulatory functions of mouse T follicular helper cells
    Authors: X Chen, Y Xu, Q Chen, H Zhang, Y Zeng, Y Geng, L Shen, F Li, L Chen, GQ Chen, C Huang, J Liu
    Nature Communications, 2022-05-19;13(1):2762.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Gremlin 1+ fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues
    Authors: VN Kapoor, S Müller, S Keerthivas, M Brown, C Chalouni, EE Storm, A Castiglion, R Lane, M Nitschke, CX Dominguez, JL Astarita, AT Krishnamur, CB Carbone, Y Senbabaogl, AW Wang, X Wu, V Cremasco, M Roose-Girm, L Tam, J Doerr, MZ Chen, WP Lee, Z Modrusan, YA Yang, R Bourgon, W Sandoval, AS Shaw, FJ de Sauvage, I Mellman, C Moussion, SJ Turley
    Nature Immunology, 2021-04-26;22(5):571-585.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  5. Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice
    Authors: Ying Wang, Thomas S Chaffee, Rebecca S LaRue, Danielle N Huggins, Patrice M Witschen, Ayman M Ibrahim et al.
    eLife
  6. Microtubules control cellular shape and coherence in amoeboid migrating cells
    Authors: Aglaja Kopf, Jörg Renkawitz, Robert Hauschild, Irute Girkontaite, Kerry Tedford, Jack Merrin et al.
    Journal of Cell Biology
  7. Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts
    Authors: Ruihua Ma, Angelica S Martínez-Ramírez, Thomas L Borders, Fanding Gao, Beatriz Sosa-Pineda
    eLife
  8. Essential Role of Canonical NF-kappa B Activity in the Development of Stromal Cell Subsets in Secondary Lymphoid Organs
    Authors: Dana Bogdanova, Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, M. Azizur Rahman, Burkhard Ludewig et al.
    The Journal of Immunology
  9. A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medulla Boundary Subcompartment of the Lymph Node
    Authors: Arata Takeuchi, Madoka Ozawa, Yasuhiro Kanda, Mina Kozai, Izumi Ohigashi, Yoichi Kurosawa et al.
    Frontiers in Immunology
  10. CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity
    J Clin Invest, 2016-05-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3
    Authors: WS Chen, Z Cao, S Sugaya, MJ Lopez, VG Sendra, N Laver, H Leffler, UJ Nilsson, J Fu, J Song, L Xia, P Hamrah, N Panjwani
    Nat Commun, 2016-04-12;7(0):11302.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Endothelial cell O-glycan deficiency causes blood/lymphatic misconnections and consequent fatty liver disease in mice.
    Authors: Fu J, Gerhardt H, McDaniel JM, Xia B, Liu X, Ivanciu L, Ny A, Hermans K, Silasi-Mansat R, McGee S, Nye E, Ju T, Ramirez MI, Carmeliet P, Cummings RD, Lupu F, Xia L
    J. Clin. Invest., 2008-10-16;118(11):3725-37.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  13. Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphoma.
    Authors: Song K, Herzog B, Sheng M, Fu J, McDaniel J, Chen H, Ruan J, Xia L
    Cancer Res, 2013-10-24;73(24):7254-64.

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