Mouse Integrin beta 2/CD18 Antibody

Catalog #: MAB2618
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2618
MAB2618-SP

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin beta 2/CD18 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin beta 2/CD18 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Integrin beta 1, beta 4, beta 6, beta 7, recombinant human (rh) Integrin beta 2 or beta 3 is observed.

Source

Monoclonal Rat IgG_2A Clone # 313903

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 2/CD18
Gln24-Asn702
Accession # P11835

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Integrin beta 2/CD18 under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 2/CD18

Integrin beta 2 is a transmembrane protein expressed by all leukocytes. It occurs in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4). These complexes are important in adhesion and leukocyte trafficking.

Entrez Gene IDs

3689 (Human); 16414 (Mouse); 309684 (Rat); 101925124 (Cynomolgus Monkey)

Alternate Names

959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; Integrin beta 2; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse Integrin beta 2/CD18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Metalloproteinase-mediated Shedding of Integrin beta2 promotes macrophage efflux from inflammatory sites.
Authors: Gomez IG, Tang J, Wilson CL, Yan W, Heinecke JW, Harlan JM, Raines EW
J. Biol. Chem., 2011-12-14;287(7):4581-9.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development, Western Blot