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Mouse IL-24 DuoSet ELISA

Catalog #: DY2786-05
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY2786-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse IL-24 ELISA Standard Curve
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse IL-24 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
7.8 - 500 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-24. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Mouse IL-24 ELISA Standard Curve View Larger

Mouse IL-24 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-24

Interleukin 24 (IL-24), also known as MDA-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. When secreted, IL-24 is a 35- to 40-kDa phosphorylated glycoprotein that may exist as either a monomer or dimer. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. IL-24 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta.

Long Name:
Interleukin 24
Entrez Gene IDs:
11009 (Human); 93672 (Mouse); 170819 (Rat)
Alternate Names:
C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein

Citations for Mouse IL-24 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Combination therapy with probiotics and anti-PD-L1 antibody synergistically ameliorates sepsis in mouse model
    Authors: Sun, L;Fang, K;Yang, Z;
    Heliyon
    Species: Mouse
    Sample Types: Serum
  2. IL-20 Cytokines Are Involved in Epithelial Lesions Associated with Virus-Induced COPD Exacerbation in Mice
    Authors: M Le Roux, A Ollivier, G Kervoaze, T Beke, L Gillet, M Pichavant, P Gosset
    Biomedicines, 2021-12-05;9(12):.
    Species: Mouse
    Sample Types: BALF
  3. Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation
    Authors: JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng
    Frontiers in Cellular Neuroscience, 2021-09-07;15(0):683687.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Acute renal injury induced by endotoxic shock in rats is alleviated via PI3K/Nrf2 pathway
    Authors: CH Chuang, CK Yang, PH Wu, Y Zhang, PJ Yang
    Eur Rev Med Pharmacol Sci, 2018-08-01;22(16):5394-5401.
    Species: Rat
    Sample Types: Tissue Homogenates
  5. Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth
    Authors: JC Klein, K Moses, G Zelinskyy, S Sody, J Buer, S Lang, I Helfrich, U Dittmer, CJ Kirschning, S Brandau
    Nat Commun, 2017-03-16;8(0):14600.
    Species: Mouse
    Sample Types: Tissue Homogenates

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