Mouse IL-23R PE-conjugated Antibody Summary
Gly24-Asp372
Accession # Q8K4B4
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑23 R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 10 µg/mL Anti-CD3 (Catalog # MAB484), 5 µg/mL Anti-CD28 (Catalog # AF483), 10 ng/mL Recombinant Mouse TGF-beta 1 (Catalog # 100-B), 20 ng/mL Recombinant Mouse IL-23 (Catalog # 1887-ML), 40 ng/mL Recombinant Mouse IL-6 (Catalog # 406-ML), and 10 ng/mL Recombinant Mouse IL-1 beta (Catalog # 401-ML) for 5 days to induce T17 activation were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse IL-23 R PE-conjugated Monoclonal Antibody (Catalog # FAB16861P) or (B) Rat IgG1Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.
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Detection of IL‑23 R in HEK293 Human Cell Line Transfected with Mouse IL-23 R by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse IL-23 R or (B) irrelevant transfectants was stained with Rat Anti-Mouse IL-23 R PE-conjugated Monoclonal Antibody (Catalog # FAB16861P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-23R
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4+ CD45RBlow memory T cells but at much lower levels by mouse CD4+ CD45RBhigh cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.
- Oppmann, B. et al. (2000) Immunity 13:715.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
- Parham, C. et al. (2002) J. Immunol. 168:5448.
- Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
- Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Product Datasheets
Citations for Mouse IL-23R PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The absence of murine cathelicidin-related antimicrobial peptide impacts host responses enhancing Salmonella enterica serovar Typhimurium infection
Authors: DM Bescucci, ST Clarke, CLJ Brown, VF Boras, T Montina, RRE Uwiera, GD Inglis
Gut pathogens, 2020-11-13;12(1):53.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
SCGN deficiency results in colitis susceptibility
Authors: Luis F Sifuentes-Dominguez, Haiying Li, Ernesto Llano, Zhe Liu, Amika Singla, Ashish S Patel et al.
eLife
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Itch inhibits IL-17-mediated colon inflammation and tumorigenesis by ROR-gammat ubiquitination.
Authors: Kathania M, Khare P, Zeng M, Cantarel B, Zhang H, Ueno H, Venuprasad K
Nat Immunol, 2016-06-20;17(8):997-1004.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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