Home / IL-23 / Mouse IL-23 DuoSet ELISA

Mouse IL-23 DuoSet ELISA

Catalog #: DY1887
26 Citations 6 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY1887
DY1887-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse IL-23 ELISA Standard Curve
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Product Details
Procedure
Citations (26)
FAQs
Supplemental Products
Reviews (6)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse IL-23 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
39.1 - 2,500 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-23. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse IL-23 ELISA Standard Curve View Larger

Mouse IL-23 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-23

IL-23 (Interleukin-23) is a disulfide-linked cytokine composed of a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. It is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens. IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. It also enhances the development of Th17 mediated autoimmunity and tumor progression. IL-23 signals through a receptor complex consisting of IL-12 R beta 1 and IL-23 R. This complex is expressed in mouse Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages and CD4+ CD45Rb(low) memory T cells.

Long Name:
Interleukin 23
Entrez Gene IDs:
51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)
Alternate Names:
IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Mouse IL-23 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. TLR2 Supports ?? T cell IL-17A Response to ocular surface commensals by Metabolic Reprogramming
    Authors: Zhu, W;Xu, X;Nagarajan, V;Guo, J;Peng, Z;Zhang, A;Liu, J;Mattapallil, MJ;Jittayasothorn, Y;Horai, R;Leger, AJS;Caspi, RR;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Protein Concentration Affects the Food Allergen ?-Conglutin Uptake and Bacteria-Induced Cytokine Production in Dendritic Cells
    Authors: Heinzl, GC;Eriksen, DB;Johnsen, PR;Scarafoni, A;Frøkiær, H;
    Biomolecules
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Agr2-associated ER stress promotes adherent-invasive E.�coli dysbiosis and triggers CD103+ dendritic cell IL-23-dependent ileocolitis
    Authors: M Viladomiu, M Khounlotha, B Dogan, SF Lima, A Elsaadi, E Cardakli, JG Castellano, C Ng, J Herzog, AA Schoenborn, M Ellermann, B Liu, S Zhang, AS Gulati, RB Sartor, KW Simpson, SM Lipkin, RS Longman
    Cell Reports, 2022-11-15;41(7):111637.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Culture Supernates, Tissue Culture Supernates
  4. The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiation
    Authors: KJ Smith, D Minns, BJ McHugh, RK Holloway, R O'Connor, A Williams, L Melrose, R McPherson, VE Miron, DJ Davidson, E Gwyer Find
    PloS Biology, 2022-08-26;20(8):e3001554.
    Species: Mouse
    Sample Types: CSF
  5. Lithocholic acid inhibits dendritic cell activation by reducing intracellular glutathione via TGR5 signaling
    Authors: J Hu, Y Zhang, S Yi, C Wang, X Huang, S Pan, J Yang, G Yuan, S Tan, H Li
    International journal of biological sciences, 2022-07-11;18(11):4545-4559.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. ADP-ribosylating adjuvant reveals plasticity in cDC1 cells that drive mucosal Th17 cell development and protection against influenza virus infection
    Authors: M Arabpour, C Lebrero-Fe, K Schön, A Strömberg, V Börjesson, K Lahl, M Ballegeer, X Saelens, D Angeletti, W Agace, N Lycke
    Mucosal Immunology, 2022-04-13;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. The phosphoinositide-3-kinase (PI3K)-delta inhibitor seletalisib impairs monocyte-derived dendritic cells maturation, APC function, and promotes their migration to CCR7 and CXCR4 ligands
    Authors: F Scopelliti, L Mercurio, C Cattani, V Dimartino, C Albanesi, G Costanzo, C Mirisola, S Madonna, A Cavani
    Journal of leukocyte biology, 2022-02-24;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Gut microbiota-mediated secondary bile acids regulate dendritic cells to attenuate autoimmune uveitis through TGR5 signaling
    Authors: J Hu, C Wang, X Huang, S Yi, S Pan, Y Zhang, G Yuan, Q Cao, X Ye, H Li
    Cell Reports, 2021-09-21;36(12):109726.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. The SNX-482 peptide from Hysterocrates gigas spider acts as an immunomodulatory molecule activating macrophages
    Authors: J Munhoz, R Thomé, A Rostami, LLW Ishikawa, L Verinaud, C Rapôso
    Peptides, 2021-09-16;146(0):170648.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Neutralization of IL-33 modifies the type 2 and type 3 inflammatory signature of viral induced asthma exacerbation
    Authors: KJ Warren, JA Poole, JM Sweeter, JM DeVasure, JD Dickinson, RS Peebles, TA Wyatt
    Respiratory Research, 2021-07-15;22(1):206.
    Species: Mouse
    Sample Types: BALF
  11. Cryptococcus gattii evades CD11b-mediated fungal recognition by coating itself with capsular polysaccharides
    Authors: K Ueno, Y Otani, N Yanagihara, M Urai, A Nagamori, M Sato-Fukus, K Shimizu, N Saito, Y Miyazaki
    European Journal of Immunology, 2021-03-30;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. CD137 Signaling Regulates Acute Colitis via RALDH2-Expressing CD11b-CD103+ DCs
    Authors: J Jin, IH Jung, SH Moon, S Jeon, SJ Jeong, SK Sonn, S Seo, MN Lee, EJ Song, HY Kweon, S Kim, TK Kim, J Kim, HR Cho, JH Choi, B Kwon, GT Oh
    Cell Rep, 2020-03-24;30(12):4124-4136.e5.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection
    Authors: AL Frisbee, MM Saleh, MK Young, JL Leslie, ME Simpson, MM Abhyankar, CA Cowardin, JZ Ma, P Pramoonjag, SD Turner, AP Liou, EL Buonomo, WA Petri
    Nat Commun, 2019-06-20;10(1):2712.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Targeting dendritic cells to accelerate T-cell activation overcomes a bottleneck in tuberculosis vaccine efficacy
    Nat Commun, 2016-12-22;7(0):13894.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Transcriptional and functional characterization of CD137L-dendritic cells identifies a novel dendritic cell phenotype
    Sci Rep, 2016-07-19;6(0):29712.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.
    Authors: Brown A, Murphy A, Lalor S, Leech J, O'Keeffe K, Mac Aogain M, O'Halloran D, Lacey K, Tavakol M, Hearnden C, Fitzgerald-Hughes D, Humphreys H, Fennell J, van Wamel W, Foster T, Geoghegan J, Lavelle E, Rogers T, McLoughlin R
    PLoS Pathog, 2015-11-05;11(11):e1005226.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. Inflammasome activation contributes to interleukin-23 production in response to Clostridium difficile.
    Authors: Cowardin C, Kuehne S, Buonomo E, Marie C, Minton N, Petri W
    MBio, 2015-01-27;6(1):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Lymphotoxin beta receptor signaling limits mucosal damage through driving IL-23 production by epithelial cells.
    Authors: Macho-Fernandez E, Koroleva E, Spencer C, Tighe M, Torrado E, Cooper A, Fu Y, Tumanov A
    Mucosal Immunol, 2014-09-03;8(2):403-13.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. IL-17 contributes to neutrophil recruitment but not to control of viral replication during acute mouse adenovirus type 1 respiratory infection.
    Authors: McCarthy M, Zhu L, Procario M, Weinberg J
    Virology, 2014-04-19;456(0):259-67.
    Species: Mouse
    Sample Types: BALF
  20. Staphylococcus aureus infection of mice expands a population of memory gammadelta T cells that are protective against subsequent infection.
    Authors: Murphy A, O'Keeffe K, Lalor S, Maher B, Mills K, McLoughlin R
    J Immunol, 2014-03-12;192(8):3697-708.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  21. Nod1 and Nod2 enhance TLR-mediated invariant NKT cell activation during bacterial infection.
    Authors: Selvanantham T, Escalante N, Cruz Tleugabulova M, Fieve S, Girardin S, Philpott D, Mallevaey T
    J Immunol, 2013-10-25;191(11):5646-54.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Influence of hypoxia-inducible factor 1-alpha on dendritic cell differentiation and migration.
    Authors: Kohler T, Reizis B, Johnson RS, Weighardt H, Forster I
    Eur. J. Immunol., 2012-05-01;42(5):1226-36.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection.
    Authors: Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C
    Infect. Immun., 2011-10-24;80(1):100-9.
    Species: Mouse
    Sample Types: Serum
  24. The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
    Authors: El-Behi M, Ciric B, Dai H
    Nat. Immunol., 2011-04-24;12(6):568-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. A critical role for C5L2 in the pathogenesis of experimental allergic asthma.
    Authors: Zhang X, Schmudde I, Laumonnier Y, Pandey MK, Clark JR, Konig P, Gerard NP, Gerard C, Wills-Karp M, Kohl J
    J. Immunol., 2010-10-25;185(11):6741-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Importance of TLR2 in the direct response of T lymphocytes to Schistosoma mansoni antigens.
    Authors: Burton OT, Gibbs S, Miller N, Jones FM, Wen L, Dunne DW, Cooke A, Zaccone P
    Eur. J. Immunol., 2010-08-01;40(8):2221-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Reviews for Mouse IL-23 DuoSet ELISA

Average Rating: 4.8 (Based on 6 Reviews)

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Mouse IL-23 DuoSet ELISA
By Anonymous on 08/10/2019
Sample Tested: Cell Lysates
Mouse IL-23 DuoSet ELISA DY1887
Mouse IL-23 DuoSet ELISA
By ahmed elshikha on 02/08/2019
Sample Tested: Cell culture supernatant,Serum and Plasma
Mouse IL-23 DuoSet ELISA
By Anonymous on 10/08/2018
Sample Tested: Serum
Mouse IL-23 DuoSet ELISA DY1887
Mouse IL-23 DuoSet ELISA
By Anonymous on 02/27/2018
Sample Tested: BMDC
Mouse IL-23 DuoSet ELISA DY1887
Mouse IL-23 DuoSet ELISA
By Anonymous on 08/24/2017
Sample Tested: Bone marrow-derived dendritic cells
Mouse IL-23 DuoSet ELISA DY1887
Mouse IL-23 DuoSet ELISA
By Anonymous on 05/27/2016
Sample Tested: Cell culture supernatant

The ELISA kit itself had a pretty lengthy and involved procedure in terms of incubation times and extra washes compared to most kits I have used. Overnight coating with primary,binding, then probing with a biotinylated capture antibody and then a detection step with streptavidin-HRP. Not as quick as other kits, however the signal/noise was excellent.

Mouse IL-23 DuoSet ELISA DY1887