Home / IL-12 p70 / Mouse IL-12 p70 DuoSet ELISA

Mouse IL-12 p70 DuoSet ELISA

Catalog #: DY419
84 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY419
DY419-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse IL-12 p70 ELISA Standard Curve
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Product Details
Procedure
Citations (84)
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Supplemental Products
Reviews (5)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse IL-12 p70 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
39.1 - 2,500 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-12 p70. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Mouse IL-12 p70 ELISA Standard Curve View Larger

Mouse IL-12 p70 ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12 p70

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Long Name:
Interleukin 12 p70
Entrez Gene IDs:
3592 (Human); 16159 (Mouse); 84405 (Rat)
Alternate Names:
CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse IL-12 p70 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

84 Citations: Showing 1 - 10
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  1. Immunobiotic Ligilactobacillus salivarius FFIG58 Confers Long-Term Protection against Streptococcus pneumoniae
    Authors: Elean, M;Raya Tonetti, F;Fukuyama, K;Arellano-Arriagada, L;Namai, F;Suda, Y;Gobbato, N;Nishiyama, K;Villena, J;Kitazawa, H;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: BALF, Cell Culture Supernates
  2. Protein Concentration Affects the Food Allergen ?-Conglutin Uptake and Bacteria-Induced Cytokine Production in Dendritic Cells
    Authors: Heinzl, GC;Eriksen, DB;Johnsen, PR;Scarafoni, A;Frøkiær, H;
    Biomolecules
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Role of the IL-33/ST2 Activation Pathway in the Development of the Hepatic Fibrosis Induced by Schistosoma mansoni Granulomas in Mice
    Authors: Maggi, L;Camelo, GMA;Rocha, IC;Pereira Alves, W;Moreira, JMP;Almeida Pereira, T;Tafuri, WL;Rabelo, ÉML;Correa, A;Ecco, R;Negrão-Corrêa, DA;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Serum
  4. Metabololipidomic and proteomic profiling reveals aberrant macrophage activation and interrelated immunomodulatory mediator release during aging
    Authors: P Schädel, A Czapka, N Gebert, ID Jacobsen, A Ori, O Werz
    Aging Cell, 2023-04-26;0(0):e13856.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Investigation of the Effects of Monomeric and Dimeric Stilbenoids on Bacteria-Induced Cytokines and LPS-Induced ROS Formation in Bone Marrow-Derived Dendritic Cells
    Authors: PR Johnsen, C Pinna, L Mattio, MB Strube, M Di Nunzio, S Iametti, S Dallavalle, A Pinto, H Frøkiær
    International Journal of Molecular Sciences, 2023-02-01;24(3):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. TRPC absence induces pro-inflammatory macrophages and gut microbe disorder, sensitizing mice to colitis
    Authors: Y Lin, X Cui, Q Cao, R Bi, Y Liu, D Jing, C Yue, Q Zhao, Y Wang, S Liu, Y Su, K Formoso, S Susperregu, L Birnbaumer, M Freichel, Y Yang, L You, X Gao
    International immunopharmacology, 2022-12-31;115(0):109655.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity
    Authors: M Tomokiyo, FR Tonetti, H Yamamuro, R Shibata, K Fukuyama, N Gobbato, L Albarracin, MSR Rajoka, AKMH Kober, W Ikeda-Ohts, J Villena, H Kitazawa
    Cells, 2022-09-25;11(19):.
    Species: Mouse
    Sample Types: BALF
  8. Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation
    Authors: Y Ni, GH Wu, JJ Cai, R Zhang, Y Zheng, JQ Liu, XH Yang, X Yang, Y Shen, JM Lai, XM Ye, SJ Mo
    International journal of biological sciences, 2022-08-08;18(13):5168-5184.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Lithocholic acid inhibits dendritic cell activation by reducing intracellular glutathione via TGR5 signaling
    Authors: J Hu, Y Zhang, S Yi, C Wang, X Huang, S Pan, J Yang, G Yuan, S Tan, H Li
    International journal of biological sciences, 2022-07-11;18(11):4545-4559.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. The phosphoinositide-3-kinase (PI3K)-delta inhibitor seletalisib impairs monocyte-derived dendritic cells maturation, APC function, and promotes their migration to CCR7 and CXCR4 ligands
    Authors: F Scopelliti, L Mercurio, C Cattani, V Dimartino, C Albanesi, G Costanzo, C Mirisola, S Madonna, A Cavani
    Journal of leukocyte biology, 2022-02-24;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. IL-2/JES6-1 mAb complexes dramatically increase sensitivity to LPS through IFN-gamma production by CD25+Foxp3- T cells
    Authors: J Tomala, P Weberova, B Tomalova, Z Jiraskova, L Sivak, J Kovarova, M Kovar
    Elife, 2021-12-21;10(0):.
    Species: Mouse
    Sample Types: Serum
  12. Endangered Lymphocytes: The Effects of Alloxan and Streptozotocin on Immune Cells in Type 1 Induced Diabetes
    Authors: LAD Queiroz, JB Assis, JPT Guimarães, ESA Sousa, AC Milhomem, KKS Sunahara, A Sá-Nunes, JO Martins
    Mediators of Inflammation, 2021-10-19;2021(0):9940009.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Enhancing adoptive CD8 T cell therapy by systemic delivery of tumor associated antigens
    Authors: DE Jæhger, ML Hübbe, MK Kræmer, G Clergeaud, AV Olsen, C Stavnsbjer, MN Wiinholt, A Kjær, JR Henriksen, AE Hansen, TL Andresen
    Scientific Reports, 2021-10-05;11(1):19794.
    Species: Mouse
    Sample Types: Serum
  14. Gut microbiota-mediated secondary bile acids regulate dendritic cells to attenuate autoimmune uveitis through TGR5 signaling
    Authors: J Hu, C Wang, X Huang, S Yi, S Pan, Y Zhang, G Yuan, Q Cao, X Ye, H Li
    Cell Reports, 2021-09-21;36(12):109726.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Water-soluble polysaccharides from Pleurotus eryngii fruiting bodies, their activity and affinity for Toll-like receptor 2 and dectin-1
    Authors: CF Ellefsen, CW Wold, AL Wilkins, F Rise, ABC Samuelsen
    Carbohydrate polymers, 2021-03-26;264(0):117991.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Hepatoprotective Effect of Mixture of Dipropyl Polysulfides in Concanavalin A-Induced Hepatitis
    Authors: D Arsenijevi, B Stojanovic, J Milovanovi, A Arsenijevi, M Simic, M Pergal, I Kodranov, O Cvetkovic, D Vojvodic, E Ristanovic, D Manojlovic, M Milovanovi, N Arsenijevi
    Nutrients, 2021-03-22;13(3):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  17. Protein Digests and Pure Peptides from Chia Seed Prevented Adipogenesis and Inflammation by Inhibiting PPAR&gamma and NF-&kappaB Pathways in 3T3L-1 Adipocytes
    Authors: M Grancieri, HSD Martino, E Gonzalez d
    Nutrients, 2021-01-08;13(1):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. Lactococcus lactis subsp. Cremoris C60 restores T Cell Population in Small Intestinal Lamina Propria in Aged Interleukin-18 Deficient Mice
    Authors: S Saito, N Kakizaki, A Okuno, T Maekawa, NM Tsuji
    Nutrients, 2020-10-27;12(11):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. Immunomodulatory Effect of a Salvia plebeia R. Aqueous Extract in Forced Swimming Exercise-induced Mice
    Authors: J Shin, OK Kim, S Kim, D Bae, J Lee, J Park, W Jun
    Nutrients, 2020-07-28;12(8):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. The Role of Alveolar Macrophages in the Improved Protection against Respiratory Syncytial Virus and Pneumococcal Superinfection Induced by the Peptidoglycan of Lactobacillus rhamnosus CRL1505
    Authors: P Clua, M Tomokiyo, F Raya Tonet, MA Islam, V García Cas, G Marcial, S Salva, S Alvarez, H Takahashi, S Kurata, H Kitazawa, J Villena
    Cells, 2020-07-09;9(7):.
    Species: Mouse
    Sample Types: BALF
  21. Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis
    Authors: AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen
    Cell Rep, 2020-04-07;31(1):107471.
    Species: Mouse
    Sample Types: Stool Homogenate
  22. Nucleic Acid-Sensing Toll-Like Receptors Play a Dominant Role in Innate Immune Recognition of Pneumococci
    Authors: A Famà, A Midiri, G Mancuso, C Biondo, G Lentini, R Galbo, MM Giardina, GV De Gaetano, L Romeo, G Teti, C Beninati
    MBio, 2020-03-24;11(2):.
    Species: Mouse
    Sample Types: Tissue Lysates
  23. Genetically Modified Mouse Mesenchymal Stem Cells Expressing Non-Structural Proteins of Hepatitis C Virus Induce Effective Immune Response
    Authors: OV Masalova, EI Lesnova, RR Klimova, ED Momotyuk, VV Kozlov, AM Ivanova, OV Payushina, NN Butorina, NF Zakirova, AN Narovlyans, AV Pronin, AV Ivanov, AA Kushch
    Vaccines (Basel), 2020-02-02;8(1):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Enzyme Treatment Alters the Anti-Inflammatory Activity of the Water Extract of Wheat Germ In Vitro and In Vivo
    Authors: Y Song, HY Jeong, JK Lee, YS Choi, DO Kim, D Jang, CS Park, S Maeng, H Kang
    Nutrients, 2019-10-16;11(10):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Syk-dependent glycolytic reprogramming in dendritic cells regulates IL-1 β production to β-glucan ligands in a TLR-independent manner
    Authors: PM Thwe, DI Fritz, JP Snyder, PR Smith, KD Curtis, A O'Donnell, NA Galasso, LA Sepaniac, BJ Adamik, LR Hoyt, PD Rodriguez, TC Hogan, AF Schmidt, ME Poynter, E Amiel
    J. Leukoc. Biol., 2019-09-11;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Discovery of a Novel Microtubule Targeting Agent as an Adjuvant for Cancer Immunotherapy
    Authors: F Sato-Kanek, X Wang, S Yao, T Hosoya, FS Lao, K Messer, M Pu, NM Shukla, HB Cottam, M Chan, DA Carson, M Corr, T Hayashi
    Biomed Res Int, 2018-10-10;2018(0):8091283.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis
    Authors: Y Fujita, A Khateb, Y Li, R Tinoco, T Zhang, H Bar-Yoseph, MA Tam, Y Chowers, E Sabo, S Gerassy-Va, E Starosvets, B James, K Brown, SS Shen-Orr, LM Bradley, PA Tessier, ZA Ronai
    Cell Rep, 2018-09-18;24(12):3296-3311.e6.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. Improving therapeutic efficacy of IL-12 intratumoral gene electrotransfer through novel plasmid design and modified parameters
    Authors: C Burkart, A Mukhopadhy, SA Shirley, RJ Connolly, JH Wright, A Bahrami, JS Campbell, RH Pierce, DA Canton
    Gene Ther., 2018-03-09;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. Activation of macrophage mediated host defense againstSalmonella typhimuriumbyMorus albaL
    Authors: B Chang, B Koo, H Lee, JS Oh, S Kim
    Food Nutr Res, 2018-02-21;62(0):.
    Species: Mouse
    Sample Types: Serum
  30. Natural killer cell-intrinsic type I IFN signaling controls Klebsiella pneumoniae growth during lung infection
    Authors: M Ivin, A Dumigan, FN de Vasconc, F Ebner, M Borroni, A Kavirayani, KN Przybyszew, RJ Ingram, S Lienenklau, U Kalinke, D Stoiber, JA Bengoechea, P Kovarik
    PLoS Pathog., 2017-11-07;13(11):e1006696.
    Species: Mouse
    Sample Types: Tissue Homogenates
  31. Erythroid Suppressor Cells Compromise Neonatal Immune Response against Bordetella pertussis
    Authors: G Dunsmore, N Bozorgmehr, C Delyea, P Koleva, A Namdar, S Elahi
    J. Immunol., 2017-08-04;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  32. TLR7 and TLR3 Sense Brucella abortus RNA to Induce Proinflammatory Cytokine Production but They Are Dispensable for Host Control of Infection
    Authors: PC Campos, MT Gomes, ES Guimarães, G Guimarães, SC Oliveira
    Front Immunol, 2017-01-23;8(0):28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  33. N-Dihydrogalactochitosan as a potent immune activator for dendritic cells
    Authors: Ahmed El-Hussein
    J Biomed Mater Res A, 2017-01-10;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  34. NLRP12 negatively regulates proinflammatory cytokine production and host defense against Brucella abortus
    Eur. J. Immunol., 2016-12-05;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  35. Polarization of macrophages towards M2 phenotype is favored by reduction in iPLA2?
    J Biol Chem, 2016-09-20;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  36. Poly-N-acetyl glucosamine production by Staphylococcus epidermidis cells increases their in vivo pro-inflammatory effect
    Infect Immun, 2016-09-19;0(0):.
    Species: Mouse
    Sample Types: Tissue Secretion
  37. Proinflammatory M1 macrophages inhibit RANKL-induced osteoclastogenesis
    Infect Immun, 2016-09-19;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. Eriobotrya japonica Water Extract Characterization: An Inducer of Interferon-Gamma Production Mainly by the JAK-STAT Pathway
    Authors: Khalid Z Matalka
    Molecules, 2016-06-02;21(6):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.
    Authors: Brown A, Murphy A, Lalor S, Leech J, O'Keeffe K, Mac Aogain M, O'Halloran D, Lacey K, Tavakol M, Hearnden C, Fitzgerald-Hughes D, Humphreys H, Fennell J, van Wamel W, Foster T, Geoghegan J, Lavelle E, Rogers T, McLoughlin R
    PLoS Pathog, 2015-11-05;11(11):e1005226.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. IL-10 Production in Macrophages Is Regulated by a TLR-Driven CREB-Mediated Mechanism That Is Linked to Genes Involved in Cell Metabolism.
    Authors: Sanin D, Prendergast C, Mountford A
    J Immunol, 2015-06-26;195(3):1218-32.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. Simultaneous and dose dependent melanoma cytotoxic and immune stimulatory activity of betulin.
    Authors: Pfarr K, Danciu C, Arlt O, Neske C, Dehelean C, Pfeilschifter J, Radeke H
    PLoS ONE, 2015-03-10;10(3):e0118802.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  42. Consistent inhibition of cyclooxygenase drives macrophages towards the inflammatory phenotype.
    Authors: Na Y, Yoon Y, Son D, Jung D, Gu G, Seok S
    PLoS ONE, 2015-02-13;10(2):e0118203.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. No Impairment in host defense against Streptococcus pneumoniae in obese CPEfat/fat mice.
    Authors: Mancuso P, O Brien E, Prano J, Goel D, Aronoff D
    PLoS ONE, 2014-09-09;9(9):e106420.
    Species: Mouse
    Sample Types: BALF
  44. MicroRNA-29b modulates innate and antigen-specific immune responses in mouse models of autoimmunity.
    Authors: Salama A, Fichou N, Allard M, Dubreil L, De Beaurepaire L, Viel A, Jegou D, Bosch S, Bach J
    PLoS ONE, 2014-09-09;9(9):e106153.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  45. Lifetime-dependent effects of bisphenol A on asthma development in an experimental mouse model.
    Authors: Petzold, Susanne, Averbeck, Marco, Simon, Jan C, Lehmann, Irina, Polte, Tobias
    PLoS ONE, 2014-06-20;9(6):e100468.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. The glycosylated Rv1860 protein of mycobacterium tuberculosis inhibits dendritic cell mediated TH1 and TH17 polarization of T cells and abrogates protective immunity conferred by BCG.
    Authors: Satchidanandam V, Kumar N, Jumani R, Challu V, Elangovan S, Khan N
    PLoS Pathog, 2014-06-12;10(6):e1004176.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  47. Role of the aryl hydrocarbon receptor in the immune response profile and development of pathology during Plasmodium berghei Anka infection.
    Authors: Brant F, Miranda A, Esper L, Rodrigues D, Kangussu L, Bonaventura D, Soriani F, Pinho V, Souza D, Rachid M, Weiss L, Tanowitz H, Teixeira M, Teixeira A, Machado F
    Infect Immun, 2014-05-12;82(8):3127-40.
    Species: Mouse
    Sample Types: Tissue Homogenates
  48. Assessing the role of STAT3 in DC differentiation and autologous DC immunotherapy in mouse models of GBM.
    Authors: Assi H, Espinosa J, Suprise S, Sofroniew M, Doherty R, Zamler D, Lowenstein P, Castro M
    PLoS ONE, 2014-05-07;9(5):e96318.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  49. Dichloroacetate modulates cytokines toward T helper 1 function via induction of the interleukin-12-interferon-gamma pathway.
    Authors: Badr M, Qinna N, Qadan F, Matalka K
    Onco Targets Ther, 2014-02-07;7(0):193-201.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  50. Endogenous oils derived from human adipocytes are potent adjuvants that promote IL-1?-dependent inflammation.
    Authors: Tynan, Graham A, Hearnden, Claire H, Oleszycka, Ewa, Lyons, Claire L, Coutts, Graham, O'Connell, Jean, Corrigan, Michelle, Lynch, Lydia, Campbell, Matthew, Callanan, John J, Mok, Kenneth, Geoghegan, Justin, O'Farrelly, Cliona, Allan, Stuart M, Roche, Helen M, O'Shea, Donal B, Lavelle, Ed C
    Diabetes, 2014-01-23;63(6):2037-50.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  51. Intraperitoneal prophylaxis with CpG oligodeoxynucleotides protects neutropenic mice against intracerebral Escherichia coli K1 infection.
    Authors: Ribes S, Meister T, Ott M, Redlich S, Janova H, Hanisch U, Nessler S, Nau R
    J Neuroinflammation, 2014-01-23;11(0):14.
    Species: Mouse
    Sample Types: Tissue Homogenates
  52. Transcriptional analysis of murine macrophages infected with different Toxoplasma strains identifies novel regulation of host signaling pathways.
    Authors: Melo M, Nguyen Q, Cordeiro C, Hassan M, Yang N, McKell R, Rosowski E, Julien L, Butty V, Darde M, Ajzenberg D, Fitzgerald K, Young L, Saeij J
    PLoS Pathog, 2013-12-19;9(12):e1003779.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  53. Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice.
    Authors: Chen Y, Chen M
    PLoS ONE, 2013-11-11;8(11):e78789.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  54. Nod1 and Nod2 enhance TLR-mediated invariant NKT cell activation during bacterial infection.
    Authors: Selvanantham T, Escalante N, Cruz Tleugabulova M, Fieve S, Girardin S, Philpott D, Mallevaey T
    J Immunol, 2013-10-25;191(11):5646-54.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  55. Adenosine augments IL-10-induced STAT3 signaling in M2c macrophages.
    Authors: Koscso B, Csoka B, Kokai E, Nemeth Z, Pacher P, Virag L, Leibovich S, Hasko G
    J Leukoc Biol, 2013-08-06;94(6):1309-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. Toll-like receptor 6 plays an important role in host innate resistance to Brucella abortus infection in mice.
    Authors: de Almeida L, Macedo G, Marinho F, Gomes M, Corsetti P, Silva A, Cassataro J, Giambartolomei G, Oliveira S
    Infect Immun, 2013-03-04;81(5):1654-62.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  57. UNC93B1 and nucleic acid-sensing Toll-like receptors mediate host resistance to infection with Leishmania major.
    Authors: Schamber-Reis B, Petritus P, Caetano B, Martinez E, Okuda K, Golenbock D, Scott P, Gazzinelli R
    J Biol Chem, 2013-01-16;288(10):7127-36.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  58. Role of OGR1 in myeloid-derived cells in prostate cancer.
    Authors: Yan, L, Singh, L S, Zhang, L, Xu, Y
    Oncogene, 2012-12-10;33(2):157-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  59. Intravenous mesenchymal stem cells prevented rejection of allogeneic corneal transplants by aborting the early inflammatory response.
    Authors: Oh J, Lee R, Yu J, Ko J, Lee H, Ko A, Roddy G, Prockop D
    Mol Ther, 2012-08-28;20(11):2143-52.
    Species: Mouse
    Sample Types: Tissue Homogenates
  60. OX40 ligand and programmed cell death 1 ligand 2 expression on inflammatory dendritic cells regulates CD4 T cell cytokine production in the lung during viral disease.
    Authors: Wythe SE, Dodd JS, Openshaw PJ, Schwarze J
    J. Immunol., 2012-01-20;188(4):1647-55.
    Species: Mouse
    Sample Types: BALF
  61. Intestinal epithelial cell-derived semaphorin 7A negatively regulates development of colitis via alphavbeta1 integrin.
    Authors: Kang S, Okuno T, Takegahara N, Takamatsu H, Nojima S, Kimura T, Yoshida Y, Ito D, Ohmae S, You DJ, Toyofuku T, Jang MH, Kumanogoh A
    J. Immunol., 2011-12-23;188(3):1108-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  62. Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection.
    Authors: Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C
    Infect. Immun., 2011-10-24;80(1):100-9.
    Species: Mouse
    Sample Types: Serum
  63. A critical role for C5L2 in the pathogenesis of experimental allergic asthma.
    Authors: Zhang X, Schmudde I, Laumonnier Y, Pandey MK, Clark JR, Konig P, Gerard NP, Gerard C, Wills-Karp M, Kohl J
    J. Immunol., 2010-10-25;185(11):6741-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  64. Th17 cells are the dominant T cell subtype primed by Shigella flexneri mediating protective immunity.
    Authors: Sellge G, Magalhaes JG, Konradt C, Fritz JH, Salgado-Pabon W, Eberl G, Bandeira A, Di Santo JP, Sansonetti PJ, Phalipon A
    J. Immunol., 2010-01-20;184(4):2076-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  65. Critical role of the IFN-stimulated gene factor 3 complex in TLR-mediated IL-27p28 gene expression revealing a two-step activation process.
    Authors: Molle C, Goldman M, Goriely S
    J. Immunol., 2010-01-18;184(4):1784-92.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  66. A conjugated linoleic acid-enriched beef diet attenuates lipopolysaccharide-induced inflammation in mice in part through PPARgamma-mediated suppression of toll-like receptor 4.
    Authors: Reynolds CM, Draper E, Keogh B, Rahman A, Moloney AP, Mills KH, Loscher CE, Roche HM
    J. Nutr., 2009-10-21;139(12):2351-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  67. E-prostanoid 3 receptor deletion improves pulmonary host defense and protects mice from death in severe Streptococcus pneumoniae infection.
    Authors: Aronoff DM, Lewis C, Serezani CH, Eaton KA, Goel D, Phipps JC, Peters-Golden M, Mancuso P
    J. Immunol., 2009-07-27;183(4):2642-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  68. The IFN regulatory factor 7-dependent type I IFN response is not essential for early resistance against murine cytomegalovirus infection.
    Authors: Steinberg C, Eisenacher K, Gross O, Reindl W, Schmitz F, Ruland J, Krug A
    Eur. J. Immunol., 2009-04-01;39(4):1007-18.
    Species: Mouse
    Sample Types: Serum
  69. CD40L-expressing CD8 T cells prime CD8alpha(+) DC for IL-12p70 production.
    Authors: Wong KL, Lew FC, MacAry PA, Kemeny DM
    Eur. J. Immunol., 2008-08-01;38(8):2251-62.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  70. Direct B cell stimulation by dendritic cells in a mouse model of lupus.
    Authors: Wan S, Zhou Z, Duan B, Morel L
    Arthritis Rheum., 2008-06-01;8(0):1741-50.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  71. Expression of bioactive single-chain murine IL-12 in transgenic plants.
    Authors: Liu J, Dolan MC, Reidy M, Cramer CL
    J. Interferon Cytokine Res., 2008-06-01;28(0):381-92.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  72. Administration of a synthetic TLR4 agonist protects mice from pneumonic tularemia.
    Authors: Lembo A, Pelletier M, Iyer R, Timko M, Dudda JC, West TE, Wilson CB, Hajjar AM, Skerrett SJ
    J. Immunol., 2008-06-01;180(11):7574-81.
    Species: Mouse
    Sample Types: Tissue Homogenates
  73. Mycobacterium tuberculosis antigens specifically modulate CCR2 and MCP-1/CCL2 on lymphoid cells from human pulmonary hilar lymph nodes.
    Authors: Arias MA, Jaramillo G, Lopez YP, Mejia N, Mejia C, Pantoja AE, Shattock RJ, Garcia LF, Griffin GE
    J. Immunol., 2007-12-15;179(12):8381-91.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Lung dendritic cells elicited by Fms-like tyrosine 3-kinase ligand amplify the lung inflammatory response to lipopolysaccharide.
    Authors: von Wulffen W, Steinmueller M, Herold S, Marsh LM, Bulau P, Seeger W, Welte T, Lohmeyer J, Maus UA
    Am. J. Respir. Crit. Care Med., 2007-08-09;176(9):892-901.
    Species: Mouse
    Sample Types: BALF
  75. Lactoferrin enhanced efficacy of the BCG vaccine to generate host protective responses against challenge with virulent Mycobacterium tuberculosis.
    Authors: Hwang SA, Wilk KM, Budnicka M, Olsen M, Bangale YA, Hunter RL, Kruzel ML, Actor JK
    Vaccine, 2007-07-27;25(37):6730-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  76. Immunotherapy for neuroblastoma using syngeneic fibroblasts transfected with IL-2 and IL-12.
    Authors: Barker SE, Grosse SM, Siapati EK, Kritz A, Kinnon C, Thrasher AJ, Hart SL
    Br. J. Cancer, 2007-06-26;97(2):210-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  77. Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity.
    Authors: Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ
    Immunity, 2007-04-12;26(4):445-59.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  78. Role of Toll-like receptor 2 in recognition of Legionella pneumophila in a murine pneumonia model.
    Authors: Fuse ET, Tateda K, Kikuchi Y, Matsumoto T, Gondaira F, Azuma A, Kudoh S, Standiford TJ, Yamaguchi K
    J. Med. Microbiol., 2007-03-01;56(0):305-12.
    Species: Mouse
    Sample Types: Tissue Homogenates
  79. Glucose-6-phosphate dehydrogenase deficiency and the inflammatory response to endotoxin and polymicrobial sepsis.
    Authors: Wilmanski J, Villanueva E, Deitch EA, Spolarics Z
    Crit. Care Med., 2007-02-01;35(2):510-8.
    Species: Mouse
    Sample Types: Plasma
  80. Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
    Authors: Carpenter KJ, Hogaboam CM
    Infect. Immun., 2005-11-01;73(11):7198-207.
    Species: Mouse
    Sample Types: Tissue Homogenates
  81. Leukemia inhibitory factor is linked to regulatory transplantation tolerance.
    Authors: Metcalfe SM, Watson TJ, Shurey S, Adams E, Green CJ
    Transplantation, 2005-03-27;79(6):726-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  82. Cytokine production in association with phagocytosis of apoptotic cells by immature dendritic cells.
    Authors: Takahashi M, Kobayashi Y
    Cell. Immunol., 2003-12-01;226(2):105-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  83. Intracellular thiols contribute to Th2 function via a positive role in IL-4 production.
    Authors: Monick MM, Samavati L, Butler NS, Mohning M, Powers LS, Yarovinsky T, Spitz DR, Hunninghake GW
    J. Immunol., 2003-11-15;171(10):5107-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  84. Reversal of tumor-induced dendritic cell paralysis by CpG immunostimulatory oligonucleotide and anti-interleukin 10 receptor antibody.
    Authors: O'Garra A
    J. Exp. Med., 2002-08-19;196(4):541-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Reviews for Mouse IL-12 p70 DuoSet ELISA

Average Rating: 4.6 (Based on 5 Reviews)

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Mouse IL-12 p70 DuoSet ELISA
By Anonymous on 12/16/2021
Sample Tested: mouse serum
Mouse IL-12 p70 DuoSet ELISA
By Anonymous on 09/26/2020
Sample Tested: Tissue Lysates
Mouse IL-12 p70 DuoSet ELISA DY419
Mouse IL-12 p70 DuoSet ELISA
By Anonymous on 11/02/2017
Sample Tested: Cell culture supernatant
Mouse IL-12 p70 DuoSet ELISA
By Anonymous on 05/06/2017
Sample Tested: Cell culture supernatant
Mouse IL-12 p70 DuoSet ELISA DY419
Mouse IL-12 p70 DuoSet ELISA
By Juwen DuBois on 04/18/2017
Sample Tested: Serum

Very consistent results. Follow manufacturers protocol.

Mouse IL-12 p70 DuoSet ELISA DY419