Home / IL-11 R alpha / Mouse IL-11 R alpha Antibody

Mouse IL-11 R alpha Antibody

Catalog #: AF490
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF490
AF490-SP
Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 R alpha Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-11 R alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-11 R alpha in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-11 R alpha
Ser22-Gln367
Accession # Q64385
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑11 R alpha Fc Chimera (Catalog # 490-IR)
Neutralization
Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. Nordan, R. P. et al. (1987) J. Immunol. 139:813. The Neutralization Dose (ND50) is typically 1-6 µg/mL in the presence of 1 ng/mL Recombinant Mouse IL‑11.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 R alpha Antibody. View Larger

Cell Proliferation Induced by IL‑11 and Neutralization by Mouse IL‑11 R alpha Antibody. Recombinant Mouse IL-11 (Catalog # 418-ML) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-11 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-11 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF490). The ND50 is typically 1-6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-11 R alpha

Mouse interleukin 11 receptor alpha (IL-11 R alpha ), originally designated NR1, is a member of the hematopoietic cytokine receptor family that was cloned on the basis of its conserved WSXWS motif. IL-11 R alpha cDNA encodes a 432 amino acid (aa) residue precursor type I membrane protein with a 23 aa residue signal peptide, a 344 aa residue extracellular domain containing two potential glycosylation sites, a 26 aa residue transmembrane region and a short (39 aa residue) cytoplasmic domain. In comparison with other hematopoietic cytokine receptors, the extracellular domain of IL-11 R alpha is closely related to that of IL-6 R alpha, CNTF R alpha and the p40 subunit of IL‑12, exhibiting 24%, 22% and 16% amino acid sequence identity, respectively. By itself, IL-11 R alpha binds IL-11 with low affinity. IL-11 R alpha, together with gp130, forms a functional high-affinity receptor complex for IL-11. The expression of IL-11 R alpha has been detected in all adult tissues examined (lung, stomach, intestine), during embryonic development and in totipotent and differentiating embryonic stem cells. Recombinant soluble IL-11 R alpha has been shown to mediate IL-11 responsiveness in cells expressing the gp130 molecule. In cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha has been shown to act as an IL-11 antagonist.

References
  1. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immuno. 15:797.
  2. Hilton, D. et al. (1994) EMBO J. 13:4765.
  3. Davidson, A.J. et al. (1997) Stem Cells 15:119.
  4. Curtis, D.J. et al. (1997) Blood 90:4403.
Long Name
Interleukin 11 Receptor alpha
Entrez Gene IDs
3590 (Human); 16157 (Mouse)
Alternate Names
AI314697; GP130; IL-11 R alpha; IL11R alpha; IL11RA; IL-11Ra; Il11ra2; NR1

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Citations for Mouse IL-11 R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Interleukin-11 regulates the fate of adipose-derived mesenchymal stem cells via STAT3 signalling pathways
    Authors: Wenlong Yang, Shuning Zhang, Tiantong Ou, Hao Jiang, Daile Jia, Zhiyong Qi et al.
    Cell Proliferation
  2. Ascl2-Dependent Cell Dedifferentiation Drives Regeneration of Ablated Intestinal Stem Cells
    Authors: K Murata, U Jadhav, S Madha, J van Es, J Dean, A Cavazza, K Wucherpfen, F Michor, H Clevers, RA Shivdasani
    Cell Stem Cell, 2020-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  3. Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes.
    Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR
    Cell. Signal., 2008-10-12;21(1):151-60.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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