Home / IgG2A / Mouse IgG2A PE-conjugated Antibody

Mouse IgG2A PE-conjugated Antibody

Catalog #: IC003P
34 Citations 10 Reviews Datasheet / COA / SDS
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Detection of Mouse IgG Isotype Control by Flow Cytometry
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Citations (34)
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Reviews (10)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IgG2A PE-conjugated Antibody Summary

Specificity
Selected for use as mouse IgG2A isotype control for flow cytometry applications.
Source
Monoclonal Mouse IgG2A Clone # 20102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Mouse IgG Isotype Control by Flow Cytometry View Larger

Detection of Mouse IgG Control by Flow Cytometry HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin a2/CD49b PE-conjugated Monoclonal Antibody (Catalog # FAB1233P, filled histogram) or Mouse IgG PE-conjugated Isotype Control Antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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Citations for Mouse IgG2A PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Metabolic Phenotypes of Response to Vaccination in Humans
    Authors: S Li, NL Sullivan, N Rouphael, T Yu, S Banton, MS Maddur, M McCausland, C Chiu, J Canniff, S Dubey, K Liu, V Tran, T Hagan, S Duraisingh, A Wieland, AK Mehta, JA Whitaker, S Subramania, DP Jones, A Sette, K Vora, A Weinberg, MJ Mulligan, HI Nakaya, M Levin, R Ahmed, B Pulendran
    Cell, 2017-05-11;169(5):862-877.e17.
  2. Co-inhibition of adenosine 2b receptor and programmed death-ligand 1 promotes the recruitment and cytotoxicity of natural killer cells in oral squamous cell carcinoma
    Authors: Wang, B;Wang, T;Yang, C;Nan, Z;Ai, D;Wang, X;Wang, H;Qu, X;Wei, F;
    PeerJ
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  3. Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer
    Authors: Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS;
    Oncology letters
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. ALDH1L2 regulation of formate, formyl-methionine, and ROS controls cancer cell migration and metastasis
    Authors: Hennequart, M;Pilley, SE;Labuschagne, CF;Coomes, J;Mervant, L;Driscoll, PC;Legrave, NM;Lee, Y;Kreuzaler, P;Macintyre, B;Panina, Y;Blagih, J;Stevenson, D;Strathdee, D;Schneider-Luftman, D;Gr�nroos, E;Cheung, EC;Yuneva, M;Swanton, C;Vousden, KH;
    Cell reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Antineoplastic efficacy profiles of avapritinib and nintedanib in KIT D816V+ systemic mastocytosis: a preclinical study
    Authors: L Degenfeld-, S Gamperl, G Stefanzl, AK Schruef, I Sadovnik, K Bauer, D Smiljkovic, G Eisenwort, B Peter, G Greiner, E Hadzijusuf, J Schwaab, WR Sperr, G Hoermann, S Kopanja, Z Szépfalusi, K Hoetzeneck, P Jaksch, A Reiter, M Arock, P Valent
    American journal of cancer research, 2023-02-15;13(2):355-378.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Interference with pathways activated by topoisomerase inhibition alters the surface expression of PD-L1 and MHC I in colon cancer cells
    Authors: M Hassan, V Trung, D Bedi, S Shaddox, D Gunturu, C Yates, P Datta, T Samuel
    Oncology Letters, 2022-12-09;25(1):41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus
    Authors: JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich
    Cellular Signalling, 2022-05-21;96(0):110360.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. CD63-mediated cloaking of VEGF in small extracellular vesicles contributes to anti-VEGF therapy resistance
    Authors: S Ma, LS Mangala, W Hu, E Bayaktar, A Yokoi, W Hu, S Pradeep, S Lee, PD Piehowski, A Villar-Pra, SY Wu, MH McGuire, OD Lara, C Rodriguez-, CJ LaFargue, NB Jennings, KD Rodland, T Liu, V Kundra, PT Ram, S Ramakrishn, G Lopez-Bere, RL Coleman, AK Sood
    Cell Reports, 2021-08-17;36(7):109549.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  9. CD40- and CD95-specific antibody single chain-Baff fusion proteins display BaffR-, TACI- and BCMA-restricted agonism
    Authors: Johannes Nelke, Juliane Medler, Daniela Weisenberger, Andreas Beilhack, Harald Wajant
    mAbs
  10. Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections
    Authors: Anne-Claire Lagrée, Fabienne Fasani, Clotilde Rouxel, Marine Pivet, Marie Pourcelot, Aurore Fablet et al.
    International Journal of Molecular Sciences
  11. Plasmacytoid dendritic cell and myeloid dendritic cell function in ageing: A comparison between elderly and young adult women
    Authors: Marloes van van Splunter, Olaf Perdijk, Henriëtte Fick-Brinkhof, Esther G. Floris-Vollenbroek, Ben Meijer, Sylvia Brugman et al.
    PLOS ONE
  12. Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line
    Authors: N Malerba, P Benzoni, GM Squeo, R Milanesi, F Giannetti, LG Sadleir, G Poke, B Augello, AI Croce, A Barbuti, G Merla
    Stem Cell Res, 2019-08-22;40(0):101547.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Bovine Lactoferrin Enhances TLR7-Mediated Responses in Plasmacytoid Dendritic Cells in Elderly Women: Results From a Nutritional Intervention Study With Bovine Lactoferrin, GOS and Vitamin D
    Authors: Marloes van Splunter, Olaf Perdijk, Henriëtte Fick-Brinkhof, Anouk L. Feitsma, Esther G. Floris-Vollenbroek, Ben Meijer et al.
    Frontiers in Immunology
  14. Derivation of human induced pluripotent stem cell line EURACi004-A from skin fibroblasts of a patient with Arrhythmogenic Cardiomyopathy carrying the heterozygous PKP2 mutation c.2569_3018del50
    Authors: B Ermon, CB Volpato, G Cattelan, R Silipigni, M Di Segni, C Cantaloni, M Casella, PP Pramstalle, G Pompilio, E Sommariva, V Meraviglia, A Rossini
    Stem Cell Res, 2018-09-06;32(0):78-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Isotype Control
  15. Simultaneous targeting of EGFR, HER2 and HER4 by afatinib overcomes intrinsic and acquired cetuximab resistance in head and neck squamous cell carcinoma cell lines
    Authors: I De Pauw, F Lardon, J Van den Bo, H Baysal, E Fransen, V Deschoolme, P Pauwels, M Peeters, JB Vermorken, A Wouters
    Mol Oncol, 2018-05-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A) from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene
    Authors: V Meraviglia, P Benzoni, S Landi, C Murano, M Langione, BM Motta, S Baratto, R Silipigni, M Di Segni, PP Pramstalle, D DiFrancesc, E Gazzerro, A Barbuti, A Rossini
    Stem Cell Res, 2017-12-18;27(0):25-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. ERBB signaling attenuates proinflammatory activation of nonclassical monocytes
    Authors: Sergey Ryzhov, Anton Matafonov, Cristi L. Galindo, Qinkun Zhang, Truc-Linh Tran, Daniel J. Lenihan et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  18. The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals
    Authors: Y Wang, X Ying, H Xu, H Yan, X Li, H Tang
    Int J Nanomedicine, 2017-02-17;12(0):1369-1384.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Isotype Control
  19. Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand
    PLoS Pathog, 2016-12-01;12(12):e1006015.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015-08-04;10(8):e0134568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Profiling of the three circulating monocyte subpopulations in human obesity.
    Authors: Devevre E, Renovato-Martins M, Clement K, Sautes-Fridman C, Cremer I, Poitou C
    J Immunol, 2015-03-18;194(8):3917-23.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  22. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015-01-01;33(1):133-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  23. Targeting chemokine receptor CXCR7 inhibits glioma cell proliferation and mobility.
    Authors: Liu Y, Carson-Walter E, Walter K
    Anticancer Res, 2015-01-01;35(1):53-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  24. Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells.
    Authors: Liu Y, Carson-Walter E, Walter K
    PLoS ONE, 2014-08-01;9(8):e103938.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. The high-affinity CXCR4 antagonist BKT140 is safe and induces a robust mobilization of human CD34+ cells in patients with multiple myeloma.
    Authors: Peled A, Abraham M, Avivi I, Rowe J, Beider K, Wald H, Tiomkin L, Ribakovsky L, Riback Y, Ramati Y, Aviel S, Galun E, Shaw H, Eizenberg O, Hardan I, Shimoni A, Nagler A
    Clin Cancer Res, 2013-11-18;20(2):469-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  26. Peripheral Type I Interferon Receptor Correlated with Oxidative Stress in Chronic Hepatitis B Virus Infection
    Authors: Jing Zhao, Yu-Chen Fan, Feng-Kai Sun, Ze-Hua Zhao, Li-Yuan Wang, Lei-Hua Hu et al.
    Journal of Interferon & Cytokine Research
  27. Dendritic cell immunoreceptor regulates Chikungunya virus pathogenesis in mice.
    Authors: Long, Kristin, Whitmore, Alan C, Ferris, Martin T, Sempowski, Gregory, McGee, Charles, Trollinger, Bianca, Gunn, Bronwyn, Heise, Mark T
    J Virol, 2013-03-13;87(10):5697-706.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  28. TNFR1 and TNFR2 regulate the extrinsic apoptotic pathway in myeloma cells by multiple mechanisms
    Authors: H Rauert, T Stühmer, R Bargou, H Wajant, D Siegmund
    Cell Death & Disease
  29. Hypoxia increases the metastatic ability of breast cancer cells via upregulation of CXCR4
    Authors: Patricia A Cronin, Jiang H Wang, H Paul Redmond
    BMC Cancer
  30. Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention.
    Authors: Taner SB, Pando MJ, Roberts A, Schellekens J, Marsh SG, Malmberg KJ, Parham P, Brodsky FM
    J. Immunol., 2010-11-29;186(1):62-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  31. Severe congenital neutropenia resulting from G6PC3 deficiency with increased neutrophil CXCR4 expression and myelokathexis.
    Authors: McDermott DH, De Ravin SS, Jun HS
    Blood, 2010-07-08;116(15):2793-802.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  32. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
    Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
    J. Immunol., 2009-03-01;182(0):3112-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  33. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009-02-07;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
    Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE
    J. Leukoc. Biol., 2004-05-20;76(2):441-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse IgG2A PE-conjugated Antibody

Average Rating: 4.8 (Based on 10 Reviews)

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Mouse IgG2A PE-conjugated Antibody
By Anonymous on 02/02/2023
Application: Iso-type Control Sample Tested: NK-92 human natural killer lymphoma cell line Species: Human
Mouse IgG2A PE-conjugated Antibody
By Anonymous on 04/12/2018
Application: Flow Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human
Mouse IgG2A PE-conjugated Antibody
By Anonymous on 03/30/2018
Application: Flow Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human
Mouse IgG2A PE-conjugated Antibody
By Anonymous on 03/23/2018
Application: Flow Sample Tested: HCT-116 human colorectal carcinoma cell line Species: Human
Flow Cytometry Mouse IgG2A PE-conjugated Antibody IC003P
Mouse IgG2A PE-conjugated Antibody
By Anonymous on 03/15/2018
Application: Flow Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human
Mouse IgG2A PE-conjugated Antibody
By Anonymous on 03/12/2018
Application: Flow Sample Tested: Jurkat human acute T cell leukemia cell line Species: Human
Mouse IgG2A PE-conjugated Antibody
By Anonymous on 11/27/2017
Application: Flow Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human
Flow Cytometry Mouse IgG2A PE-conjugated Antibody IC003P
Mouse IgG2A PE-conjugated Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Blood monocytes Species: Human
Mouse IgG2A PE-conjugated Antibody
By Susan Sheng on 06/20/2017
Application: Flow Sample Tested: Granta,Ramos human Burkitt's lymphoma cell line,RS4;11 Species: Human
Flow Cytometry Mouse IgG2A PE-conjugated Antibody IC003P
Mouse IgG2A PE-conjugated Antibody
By Anonymous on 07/27/2016
Application: Flow Sample Tested: B cells Species: Human